Investigation of long non‑coding RNA expression profiles in patients with post‑menopausal osteoporosis by RNA sequencing
Affiliations: Department of Obstetrics and Gynecology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430012, P.R. China
- Published online on: June 11, 2020 https://doi.org/10.3892/etm.2020.8881
Copyright: © Wang
et al. This is an open access article distributed under the
terms of Creative
Commons Attribution License.
Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
This article is mentioned in:
The present study aimed to investigate the implication of long non‑coding RNA (lncRNA) expression profiles in post‑menopausal osteoporosis (PMOP). A total of 10 patients with PMOP and 10 age‑matched healthy post‑menopausal females as controls were consecutively enrolled. Their peripheral blood mononuclear cells were obtained and lncRNA as well as mRNA expression profiles were detected by RNA sequencing, followed by bioinformatics analyses. The lncRNA expression profiles were able to distinguish patients with PMOP from controls based on principal component analysis and heatmap analysis. In total, 254 upregulated lncRNAs and 359 downregulated lncRNAs were identified in patients with PMOP vs. controls. The top 5 upregulated lncRNAs were RP11‑704M14.1, RP11‑754N21.1, RP11‑408E5.5, ANKRD26P3 and TPTEP1. The top 5 downregulated lncRNAs were RP11‑310E22.4, RP11‑326K13.4, FABP5P1, SERPINB9P1 and RPL13P2. Based on the interaction of dysregulated lncRNAs and mRNAs by RNA sequencing, functional annotations were then performed. Gene Ontology enrichment analysis revealed that the dysregulated lncRNAs were enriched in terms including apoptotic process and positive regulation of NF‑κB transaction, and Kyoto Encyclopedia of Genes and Genomes analysis suggested enrichment in PMOP‑associated signaling pathways, including osteoclast differentiation, tumor necrosis factor signaling pathway and mitogen‑activated protein kinase signaling pathway. In addition, the regulatory network and circos graph further indicated the implication of lncRNA expression profiles in PMOP via interactions with mRNAs. In conclusion, the present study suggested that aberrant lncRNA expression is deeply involved in the pathogenesis of PMOP by affecting osteoclast differentiation, inflammation and apoptotic processes.