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Mycobacterium tuberculosis infection increases the number of osteoclasts and inhibits osteoclast apoptosis by regulating TNF‑α‑mediated osteoclast autophagy

  • Authors:
    • Wei Liu
    • Juan Zhou
    • Fei Niu
    • Feifei Pu
    • Zhiwei Wang
    • Mi Huang
    • Xiaolong Zhao
    • Lin Yang
    • Pengfei Tao
    • Ping Xia
    • Jing Feng
  • View Affiliations / Copyright

    Affiliations: Department of Orthopaedics, First Hospital of Wuhan, Wuhan, Hubei 430022, P.R. China
    Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1889-1898
    |
    Published online on: June 18, 2020
       https://doi.org/10.3892/etm.2020.8903
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Abstract

Osteoarticular tuberculosis, a chronic inflammatory disease characterized by Mycobacterium tuberculosis (M.tb) infection, has become a serious problem in China. The present study was conducted to determine the mechanism of action of tumor necrosis factor (TNF)‑α in the pathogenesis of osteoarticular tuberculosis. The number of osteoclasts in osteoarticular tuberculosis tissue samples was detected by tartrate‑resistant acid phosphatase staining. Autophagy and apoptosis of osteoclasts were detected by western blotting, reverse transcription‑quantitative PCR, transmission electron microscopy and TUNEL staining. The results showed that autophagy and the number of osteoclasts increased in the lesions of patients with osteoarticular tuberculosis compared with osteoarthritis samples. Moreover, activation of osteoclast autophagy inhibited the apoptosis of osteoclasts infected with M.tb, and increased the expression level of TNF‑α. The results showed that TNF‑α enhanced the autophagic activity of M.tb‑infected osteoclasts and inhibited cell apoptosis. These findings indicated that M.tb infection induced osteoclast production and inhibited osteoclast apoptosis by regulating TNF‑α‑mediated osteoclast autophagy, revealing a new mechanism for TNF‑α in the pathogenesis of osteoarticular tuberculosis.
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Copy and paste a formatted citation
Spandidos Publications style
Liu W, Zhou J, Niu F, Pu F, Wang Z, Huang M, Zhao X, Yang L, Tao P, Xia P, Xia P, et al: Mycobacterium tuberculosis infection increases the number of osteoclasts and inhibits osteoclast apoptosis by regulating TNF‑α‑mediated osteoclast autophagy. Exp Ther Med 20: 1889-1898, 2020.
APA
Liu, W., Zhou, J., Niu, F., Pu, F., Wang, Z., Huang, M. ... Feng, J. (2020). Mycobacterium tuberculosis infection increases the number of osteoclasts and inhibits osteoclast apoptosis by regulating TNF‑α‑mediated osteoclast autophagy. Experimental and Therapeutic Medicine, 20, 1889-1898. https://doi.org/10.3892/etm.2020.8903
MLA
Liu, W., Zhou, J., Niu, F., Pu, F., Wang, Z., Huang, M., Zhao, X., Yang, L., Tao, P., Xia , P., Feng, J."Mycobacterium tuberculosis infection increases the number of osteoclasts and inhibits osteoclast apoptosis by regulating TNF‑α‑mediated osteoclast autophagy". Experimental and Therapeutic Medicine 20.3 (2020): 1889-1898.
Chicago
Liu, W., Zhou, J., Niu, F., Pu, F., Wang, Z., Huang, M., Zhao, X., Yang, L., Tao, P., Xia , P., Feng, J."Mycobacterium tuberculosis infection increases the number of osteoclasts and inhibits osteoclast apoptosis by regulating TNF‑α‑mediated osteoclast autophagy". Experimental and Therapeutic Medicine 20, no. 3 (2020): 1889-1898. https://doi.org/10.3892/etm.2020.8903
Copy and paste a formatted citation
x
Spandidos Publications style
Liu W, Zhou J, Niu F, Pu F, Wang Z, Huang M, Zhao X, Yang L, Tao P, Xia P, Xia P, et al: Mycobacterium tuberculosis infection increases the number of osteoclasts and inhibits osteoclast apoptosis by regulating TNF‑α‑mediated osteoclast autophagy. Exp Ther Med 20: 1889-1898, 2020.
APA
Liu, W., Zhou, J., Niu, F., Pu, F., Wang, Z., Huang, M. ... Feng, J. (2020). Mycobacterium tuberculosis infection increases the number of osteoclasts and inhibits osteoclast apoptosis by regulating TNF‑α‑mediated osteoclast autophagy. Experimental and Therapeutic Medicine, 20, 1889-1898. https://doi.org/10.3892/etm.2020.8903
MLA
Liu, W., Zhou, J., Niu, F., Pu, F., Wang, Z., Huang, M., Zhao, X., Yang, L., Tao, P., Xia , P., Feng, J."Mycobacterium tuberculosis infection increases the number of osteoclasts and inhibits osteoclast apoptosis by regulating TNF‑α‑mediated osteoclast autophagy". Experimental and Therapeutic Medicine 20.3 (2020): 1889-1898.
Chicago
Liu, W., Zhou, J., Niu, F., Pu, F., Wang, Z., Huang, M., Zhao, X., Yang, L., Tao, P., Xia , P., Feng, J."Mycobacterium tuberculosis infection increases the number of osteoclasts and inhibits osteoclast apoptosis by regulating TNF‑α‑mediated osteoclast autophagy". Experimental and Therapeutic Medicine 20, no. 3 (2020): 1889-1898. https://doi.org/10.3892/etm.2020.8903
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