Open Access

Pterostilbene reduces endothelial cell apoptosis by regulation of the Nrf2‑mediated TLR‑4/MyD88/NF‑κB pathway in a rat model of atherosclerosis

  • Authors:
    • Xiaowei Xiong
    • Weihang Lu
    • Kaihua Zhang
    • Weimin Zhou
  • View Affiliations

  • Published online on: June 24, 2020     https://doi.org/10.3892/etm.2020.8923
  • Pages: 2090-2098
  • Copyright: © Xiong et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Endothelial cell injury in vascular arterial walls plays a crucial role in the pathological process of atherosclerosis. Pterostilbene, a stilbenoid chemically related to resveratrol, has anti‑inflammatory, anti‑apoptosis and antioxidant properties. However, the underlying mechanisms mediated by pterostilbene in regards to endothelial cell injury in vascular arterial walls are not fully understood. The purpose of the present study was to investigate the benefits of pterostilbene in a rat model of atherosclerosis. The possible mechanism of pterostilbene was also analyzed in regards to endothelial cell injury in vascular arterial walls in vitro. A rat model of atherosclerosis was established using endothelial injury of the iliac arteries. CCK‑8 assay, TUNEL, immunofluorescence, western blot analysis and hematoxylin and eosin (H&E) staining were used to analyze the role of pterostilbene in the pathological processes of atherosclerosis. In vivo results showed that pterostilbene decreased cholesterol (CHO), high‑density lipoprotein cholesterol (HDL‑C), total cholesterol (TC), low‑density lipoprotein cholesterol (LDL‑C) in plasma and attenuated interleukin (IL)‑1, tumor necrosis factor (TNF)‑α and IL‑6 and oxidative stress injury in serum in the experimental animals. Pterostilbene treatment reduced atherogenesis, aortic plaques, macrophage infiltration and apoptosis of vascular arterial walls in the atherosclerosis rat model. In vitro assay demonstrated that pterostilbene administration increased viability of the endothelial cells, attenuated oxidative stress injury and apoptosis of endothelial cells. The results found that pterostilbene regulated endothelial cell apoptosis via the Nrf2‑mediated TLR‑4/MyD88/NF‑κB pathway. In conclusion, data from the present study revealed that pterostilbene protects rats against atherosclerosis by regulation of the Nrf2‑mediated TLR‑4/MyD88/NF‑κB pathway.

Related Articles

Journal Cover

September-2020
Volume 20 Issue 3

Print ISSN: 1792-0981
Online ISSN:1792-1015

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Xiong X, Lu W, Zhang K and Zhou W: Pterostilbene reduces endothelial cell apoptosis by regulation of the Nrf2‑mediated TLR‑4/MyD88/NF‑κB pathway in a rat model of atherosclerosis. Exp Ther Med 20: 2090-2098, 2020
APA
Xiong, X., Lu, W., Zhang, K., & Zhou, W. (2020). Pterostilbene reduces endothelial cell apoptosis by regulation of the Nrf2‑mediated TLR‑4/MyD88/NF‑κB pathway in a rat model of atherosclerosis. Experimental and Therapeutic Medicine, 20, 2090-2098. https://doi.org/10.3892/etm.2020.8923
MLA
Xiong, X., Lu, W., Zhang, K., Zhou, W."Pterostilbene reduces endothelial cell apoptosis by regulation of the Nrf2‑mediated TLR‑4/MyD88/NF‑κB pathway in a rat model of atherosclerosis". Experimental and Therapeutic Medicine 20.3 (2020): 2090-2098.
Chicago
Xiong, X., Lu, W., Zhang, K., Zhou, W."Pterostilbene reduces endothelial cell apoptosis by regulation of the Nrf2‑mediated TLR‑4/MyD88/NF‑κB pathway in a rat model of atherosclerosis". Experimental and Therapeutic Medicine 20, no. 3 (2020): 2090-2098. https://doi.org/10.3892/etm.2020.8923