MicroRNA‑19a‑3p inhibits the cellular proliferation and invasion of non‑small cell lung cancer by downregulating UBAP2L

  • Authors:
    • Yuejiang Pan
    • Ke Jin
    • Xuan Xie
    • Kexi Wang
    • Huizhong Zhang
  • View Affiliations

  • Published online on: June 24, 2020     https://doi.org/10.3892/etm.2020.8926
  • Pages: 2252-2261
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Abstract

MicroRNAs (miRNAs) are increasingly recognized as important regulators of non‑small cell lung cancer (NSCLC) progression by directly regulating their target genes. The aim of the present study was to assess the biological role of miR‑19a‑3p in NSCLC. It was revealed that miR‑19a‑3p expression was significantly downregulated in human NSCLC tissues and cell lines compared with normal tissues and lung epithelial cells. In addition, a lower miR‑19a‑3p expression was significantly associated with Tumor Node Metastasis stage and lymph node metastasis. Furthermore, the upregulation of miR‑19a‑3p in NSCLC cell lines significantly inhibited cell proliferation, migration and invasion, as determined using an MTT, colony formation, wound healing and transwell Matrigel invasion assays, respectively. A luciferase reporter assay and western blotting determined that ubiquitin associated protein 2 like (UBAP2L) was a direct target of miR‑19a‑3p and could be inhibited through the upregulation of miR‑19a‑3p in NSCLC. In addition, UBAP2L silencing induced similar effects to those observed following miR‑19a‑3p overexpression. The overexpression of UBAP2L partially reversed the effects of miR‑19a‑3p on NSCLC cell lines. Collectively, these data indicated that miR‑19a‑3p may serve as a tumor suppressor partly through the regulation of UBAP2L expression in NSCLC and that the targeting of miR‑19a‑3p may be a novel method for NSCLC treatment.

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September-2020
Volume 20 Issue 3

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Pan Y, Jin K, Xie X, Wang K and Zhang H: MicroRNA‑19a‑3p inhibits the cellular proliferation and invasion of non‑small cell lung cancer by downregulating UBAP2L. Exp Ther Med 20: 2252-2261, 2020
APA
Pan, Y., Jin, K., Xie, X., Wang, K., & Zhang, H. (2020). MicroRNA‑19a‑3p inhibits the cellular proliferation and invasion of non‑small cell lung cancer by downregulating UBAP2L. Experimental and Therapeutic Medicine, 20, 2252-2261. https://doi.org/10.3892/etm.2020.8926
MLA
Pan, Y., Jin, K., Xie, X., Wang, K., Zhang, H."MicroRNA‑19a‑3p inhibits the cellular proliferation and invasion of non‑small cell lung cancer by downregulating UBAP2L". Experimental and Therapeutic Medicine 20.3 (2020): 2252-2261.
Chicago
Pan, Y., Jin, K., Xie, X., Wang, K., Zhang, H."MicroRNA‑19a‑3p inhibits the cellular proliferation and invasion of non‑small cell lung cancer by downregulating UBAP2L". Experimental and Therapeutic Medicine 20, no. 3 (2020): 2252-2261. https://doi.org/10.3892/etm.2020.8926