Open Access

IL‑22/IL‑22R1 promotes proliferation and collagen synthesis of MRC‑5 cells via the JAK/STAT3 signaling pathway and regulates airway subepithelial fibrosis

  • Authors:
    • Juan Liu
    • Biao Shang
    • Jing Bai
  • View Affiliations

  • Published online on: June 24, 2020     https://doi.org/10.3892/etm.2020.8931
  • Pages: 2148-2156
  • Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Asthma in children poses a threat to their health, but the mechanism remains to be elucidated. The present study investigated the mechanism by which the interleukin (IL)‑22/IL‑22 receptor 1 (IL‑22R1) signaling pathway regulates subepithelial fibrosis in children with asthma. A total of 41 children with asthma and 12 healthy children were included in the present study. ELISA was performed to measure the content of IL‑22 in peripheral blood. Serum from children with asthma was used to incubate MRC‑5 cells and IL‑22 antibody rescued the effect of IL‑22 on the biological functions of MRC‑5 cells. Reverse transcription‑quantitative PCR was performed to determine IL‑22R1 mRNA expression levels and western blotting was performed to measure IL‑22R1 protein expression. The Cell Counting Kit‑8 assay was used to analyze cell proliferation and flow cytometry was performed to assess the cell cycle distribution of MRC‑5 cells. The expression of IL‑22 was elevated in peripheral blood from children with asthma, which promoted the proliferation of MRC‑5 cells, possibly via the upregulation of collagen type I α1 chain (COL1α1) and collagen type I α2 chain (COL1α2). IL‑22 exerted its biological functions via IL‑22R1. The IL‑22/IL‑22R1 signaling pathway regulated the proliferation of MRC‑5 cells and the expression of COL1α1 and COL1α2 in MRC‑5 cells via the JAK/STAT3 signaling pathway. Mononuclear lymphocytes from children with asthma stimulated the proliferation and secretory function of fibroblasts by secreting IL‑22. The present study suggested that IL‑22 expression in peripheral blood of children with asthma is upregulated compared with the control group. Furthermore, the present study indicated that the IL‑22/IL‑22R1 signaling pathway promoted MRC‑5 cell proliferation and collagen synthesis by activating the JAK/STAT3 signaling pathway, thereby potentially regulating airway subepithelial fibrosis.

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September-2020
Volume 20 Issue 3

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Liu J, Shang B and Bai J: IL‑22/IL‑22R1 promotes proliferation and collagen synthesis of MRC‑5 cells via the JAK/STAT3 signaling pathway and regulates airway subepithelial fibrosis. Exp Ther Med 20: 2148-2156, 2020
APA
Liu, J., Shang, B., & Bai, J. (2020). IL‑22/IL‑22R1 promotes proliferation and collagen synthesis of MRC‑5 cells via the JAK/STAT3 signaling pathway and regulates airway subepithelial fibrosis. Experimental and Therapeutic Medicine, 20, 2148-2156. https://doi.org/10.3892/etm.2020.8931
MLA
Liu, J., Shang, B., Bai, J."IL‑22/IL‑22R1 promotes proliferation and collagen synthesis of MRC‑5 cells via the JAK/STAT3 signaling pathway and regulates airway subepithelial fibrosis". Experimental and Therapeutic Medicine 20.3 (2020): 2148-2156.
Chicago
Liu, J., Shang, B., Bai, J."IL‑22/IL‑22R1 promotes proliferation and collagen synthesis of MRC‑5 cells via the JAK/STAT3 signaling pathway and regulates airway subepithelial fibrosis". Experimental and Therapeutic Medicine 20, no. 3 (2020): 2148-2156. https://doi.org/10.3892/etm.2020.8931