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circANRIL reduces vascular endothelial injury, oxidative stress and inflammation in rats with coronary atherosclerosis

  • Authors:
    • Peixia Shi
    • Hongling Ji
    • Huajuan Zhang
    • Jian Yang
    • Rui Guo
    • Jianwei Wang
  • View Affiliations / Copyright

    Affiliations: No. 1 Department of Cardiology, The People's Hospital of Zhangqiu Area, Jinan, Shandong 250200, P.R. China, Disinfection Supply Center, Jinan Zhangqiu District Hospital of Traditional Chinese Medicine, Jinan, Shandong 250200, P.R. China, Hospital-Acquired Infection Control Department, Jinan Zhangqiu District Hospital of Traditional Chinese Medicine, Jinan, Shandong 250200, P.R. China, Department of Clinical Laboratory, Τhe People's Hospital of Zhangqiu Area, Jinan, Shandong 250200, P.R. China, Department of Outpatients, Τhe People's Hospital of Zhangqiu Area, Jinan, Shandong 250200, P.R. China, ECG Room, W.F. Maternal and Child Health Hospital, Weicheng, Weifang 261000, P.R. China
    Copyright: © Shi et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2245-2251
    |
    Published online on: June 29, 2020
       https://doi.org/10.3892/etm.2020.8956
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Abstract

Effects of circular antisense non‑coding RNA in the INK4 locus (circANRIL) on vascular endothelial injury, oxidative stress and inflammation in rats with coronary atherosclerosis were studied by establishing a rat model of coronary atherosclerosis in which circANRIL was differentially expressed. A total of 40 healthy Sprague Dawley (SD) rats were randomly divided into research group (n=32) and control group (n=8). In research group, a rat model of coronary atherosclerosis was established without special treatment. The blood calcium (Ca2+) and lipid levels in the two groups were compared. After cell transfection, the rats were divided into blank group (untransfected), negative group (transfected with blank vector), circANRIL group (transfected with circANRIL overexpression plasmid) and circANRIL inhibitor group (transfected with circANRIL silencer). Then the levels of lactate dehydrogenase (LDH), superoxide dismutase (SOD), malondialdehyde (MDA), tumor necrosis factor‑α (TNF‑α) and interleukin‑6 (IL‑6) in each group were compared. Western blotting was adopted to detect the expressions of phosphorylated p38 mitogen‑activated protein kinase (p‑p38MAPK), p38MAPK and glyceraldehyde 3‑phosphate dehydrogenase (GAPDH). Finally, p‑p38MAPK/GAPDH, p38MAPK/GAPDH and p‑p38MAPK/p38MAPK were calculated. There were significant differences in the levels of serum Ca2+ and lipid between control group and research group (P<0.05). Besides, differences in LDH, SOD, MDA, TNF‑α and IL‑6 in the supernatant in each group were statistically significant (P<0.05 or P<0.01). Moreover, there were statistically significant differences in the gray values of p‑p38MAPK/GAPDH and p38MAPK/GAPDH and their ratio p‑p38MAPK/p38MAPK in each group (P<0.05 or P<0.01). Inhibiting the expression of circANRIL in coronary heart disease cases can reduce vascular endothelial injury, oxidative stress and inflammation.
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1 

Li GM, Zhang CL, Rui RP, Sun B and Guo W: Bioinformatics analysis of common differential genes of coronary artery disease and ischemic cardiomyopathy. Eur Rev Med Pharmacol Sci. 22:3553–3569. 2018.PubMed/NCBI View Article : Google Scholar

2 

Natarelli L and Weber C: Next-generation therapeutic concepts for atherosclerosis: Focus on cell specificity and noncoding RNAs. Thromb Haemost. 119:1199–1201. 2019.PubMed/NCBI View Article : Google Scholar

3 

Martini E, Stirparo GG and Kallikourdis M: Immunotherapy for cardiovascular disease. J Leukoc Biol. 103:493–500. 2018.PubMed/NCBI View Article : Google Scholar

4 

Busygina K, Denzinger V, Bernlochner I, Weber C, Lorenz R and Siess W: Btk Inhibitors as first oral atherothrombosis-selective antiplatelet drugs? Thromb Haemost. 119:1212–1221. 2019.PubMed/NCBI View Article : Google Scholar

5 

Conn SJ, Pillman KA, Toubia J, Conn VM, Salmanidis M, Phillips CA, Roslan S, Schreiber AW, Gregory PA and Goodall GJ: The RNA binding protein quaking regulates formation of circRNAs. Cell. 160:1125–1134. 2015.PubMed/NCBI View Article : Google Scholar

6 

Ivanov A, Memczak S, Wyler E, Torti F, Porath HT, Orejuela MR, Piechotta M, Levanon EY, Landthaler M, Dieterich C, et al: Analysis of intron sequences reveals hallmarks of circular RNA biogenesis in animals. Cell Rep. 10:170–177. 2015.PubMed/NCBI View Article : Google Scholar

7 

Holdt LM, Stahringer A, Sass K, Pichler G, Kulak NA, Wilfert W, Kohlmaier A, Herbst A, Northoff BH, Nicolaou A, et al: Circular non-coding RNA ANRIL modulates ribosomal RNA maturation and atherosclerosis in humans. Nat Commun. 7(12429)2016.PubMed/NCBI View Article : Google Scholar

8 

Gou SH, Liu BJ, Han XF, Wang L, Zhong C, Liang S, Liu H, Qiang Y, Zhang Y and Ni JM: Anti-atherosclerotic effect of Fermentum rubrum and Gynostemma pentaphyllum mixture in high-fat emulsion- and vitamin D3-induced atherosclerotic rats. J Chin Med Assoc. 81:398–408. 2018.PubMed/NCBI View Article : Google Scholar

9 

Qu S, Zhong Y, Shang R, Zhang X, Song W, Kjems J and Li H: The emerging landscape of circular RNA in life processes. RNA Biol. 14:992–999. 2017.PubMed/NCBI View Article : Google Scholar

10 

Salzman J, Gawad C, Wang PL, Lacayo N and Brown PO: Circular RNAs are the predominant transcript isoform from hundreds of human genes in diverse cell types. PLoS One. 7(e30733)2012.PubMed/NCBI View Article : Google Scholar

11 

Congrains A, Kamide K, Katsuya T, Yasuda O, Oguro R, Yamamoto K, Ohishi M and Rakugi H: CVD-associated non-coding RNA, ANRIL, modulates expression of atherogenic pathways in VSMC. Biochem Biophys Res Commun. 419:612–616. 2012.PubMed/NCBI View Article : Google Scholar

12 

Lo Sardo V, Chubukov P, Ferguson W, Kumar A, Teng EL, Duran M, Zhang L, Cost G, Engler AJ, Urnov F, et al: Unveiling the role of the most impactful cardiovascular risk locus through haplotype editing. Cell. 175:1796–1810.e20. 2018.PubMed/NCBI View Article : Google Scholar

13 

Jeck WR, Sorrentino JA, Wang K, Slevin MK, Burd CE, Liu J, Marzluff WF and Sharpless NE: Circular RNAs are abundant, conserved, and associated with ALU repeats. RNA. 19:141–157. 2013.PubMed/NCBI View Article : Google Scholar

14 

Hansen TB, Jensen TI, Clausen BH, Bramsen JB, Finsen B, Damgaard CK and Kjems J: Natural RNA circles function as efficient microRNA sponges. Nature. 495:384–388. 2013.PubMed/NCBI View Article : Google Scholar

15 

Han YN, Xia SQ, Zhang YY, Zheng JH and Li W: Circular RNAs: A novel type of biomarker and genetic tools in cancer. Oncotarget. 8:64551–64563. 2017.PubMed/NCBI View Article : Google Scholar

16 

Zeng X, Lin W, Guo M and Zou Q: A comprehensive overview and evaluation of circular RNA detection tools. PLOS Comput Biol. 13(e1005420)2017.PubMed/NCBI View Article : Google Scholar

17 

Burd CE, Jeck WR, Liu Y, Sanoff HK, Wang Z and Sharpless NE: Expression of linear and novel circular forms of an INK4/ARF-associated non-coding RNA correlates with atherosclerosis risk. PLoS Genet. 6(e1001233)2010.PubMed/NCBI View Article : Google Scholar

18 

Li CY, Ma L and Yu B: Circular RNA hsa_circ_0003575 regulates oxLDL induced vascular endothelial cells proliferation and angiogenesis. Biomed Pharmacother. 95:1514–1519. 2017.PubMed/NCBI View Article : Google Scholar

19 

Geng HH, Li R, Su YM, Xiao J, Pan M, Cai XX and Ji XP: The circular RNA Cdr1as promotes myocardial infarction by mediating the regulation of miR-7a on its target genes expression. PLoS One. 11(e0151753)2016.PubMed/NCBI View Article : Google Scholar

20 

Zhao Z, Li X, Gao C, Jian D, Hao P, Rao L and Li M: Peripheral blood circular RNA hsa_circ_0124644 can be used as a diagnostic biomarker of coronary artery disease. Sci Rep. 7(39918)2017.PubMed/NCBI View Article : Google Scholar

21 

Song CL, Wang JP, Xue X, Liu N, Zhang XH, Zhao Z, Liu JG, Zhang CP, Piao ZH, Liu Y, et al: Effect of circular ANRIL on the inflammatory response of vascular endothelial cells in a rat model of coronary atherosclerosis. Cell Physiol Biochem. 42:1202–1212. 2017.PubMed/NCBI View Article : Google Scholar

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Spandidos Publications style
Shi P, Ji H, Zhang H, Yang J, Guo R and Wang J: circANRIL reduces vascular endothelial injury, oxidative stress and inflammation in rats with coronary atherosclerosis. Exp Ther Med 20: 2245-2251, 2020.
APA
Shi, P., Ji, H., Zhang, H., Yang, J., Guo, R., & Wang, J. (2020). circANRIL reduces vascular endothelial injury, oxidative stress and inflammation in rats with coronary atherosclerosis. Experimental and Therapeutic Medicine, 20, 2245-2251. https://doi.org/10.3892/etm.2020.8956
MLA
Shi, P., Ji, H., Zhang, H., Yang, J., Guo, R., Wang, J."circANRIL reduces vascular endothelial injury, oxidative stress and inflammation in rats with coronary atherosclerosis". Experimental and Therapeutic Medicine 20.3 (2020): 2245-2251.
Chicago
Shi, P., Ji, H., Zhang, H., Yang, J., Guo, R., Wang, J."circANRIL reduces vascular endothelial injury, oxidative stress and inflammation in rats with coronary atherosclerosis". Experimental and Therapeutic Medicine 20, no. 3 (2020): 2245-2251. https://doi.org/10.3892/etm.2020.8956
Copy and paste a formatted citation
x
Spandidos Publications style
Shi P, Ji H, Zhang H, Yang J, Guo R and Wang J: circANRIL reduces vascular endothelial injury, oxidative stress and inflammation in rats with coronary atherosclerosis. Exp Ther Med 20: 2245-2251, 2020.
APA
Shi, P., Ji, H., Zhang, H., Yang, J., Guo, R., & Wang, J. (2020). circANRIL reduces vascular endothelial injury, oxidative stress and inflammation in rats with coronary atherosclerosis. Experimental and Therapeutic Medicine, 20, 2245-2251. https://doi.org/10.3892/etm.2020.8956
MLA
Shi, P., Ji, H., Zhang, H., Yang, J., Guo, R., Wang, J."circANRIL reduces vascular endothelial injury, oxidative stress and inflammation in rats with coronary atherosclerosis". Experimental and Therapeutic Medicine 20.3 (2020): 2245-2251.
Chicago
Shi, P., Ji, H., Zhang, H., Yang, J., Guo, R., Wang, J."circANRIL reduces vascular endothelial injury, oxidative stress and inflammation in rats with coronary atherosclerosis". Experimental and Therapeutic Medicine 20, no. 3 (2020): 2245-2251. https://doi.org/10.3892/etm.2020.8956
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