Open Access

circANRIL reduces vascular endothelial injury, oxidative stress and inflammation in rats with coronary atherosclerosis

  • Authors:
    • Peixia Shi
    • Hongling Ji
    • Huajuan Zhang
    • Jian Yang
    • Rui Guo
    • Jianwei Wang
  • View Affiliations

  • Published online on: June 29, 2020     https://doi.org/10.3892/etm.2020.8956
  • Pages: 2245-2251
  • Copyright: © Shi et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Effects of circular antisense non‑coding RNA in the INK4 locus (circANRIL) on vascular endothelial injury, oxidative stress and inflammation in rats with coronary atherosclerosis were studied by establishing a rat model of coronary atherosclerosis in which circANRIL was differentially expressed. A total of 40 healthy Sprague Dawley (SD) rats were randomly divided into research group (n=32) and control group (n=8). In research group, a rat model of coronary atherosclerosis was established without special treatment. The blood calcium (Ca2+) and lipid levels in the two groups were compared. After cell transfection, the rats were divided into blank group (untransfected), negative group (transfected with blank vector), circANRIL group (transfected with circANRIL overexpression plasmid) and circANRIL inhibitor group (transfected with circANRIL silencer). Then the levels of lactate dehydrogenase (LDH), superoxide dismutase (SOD), malondialdehyde (MDA), tumor necrosis factor‑α (TNF‑α) and interleukin‑6 (IL‑6) in each group were compared. Western blotting was adopted to detect the expressions of phosphorylated p38 mitogen‑activated protein kinase (p‑p38MAPK), p38MAPK and glyceraldehyde 3‑phosphate dehydrogenase (GAPDH). Finally, p‑p38MAPK/GAPDH, p38MAPK/GAPDH and p‑p38MAPK/p38MAPK were calculated. There were significant differences in the levels of serum Ca2+ and lipid between control group and research group (P<0.05). Besides, differences in LDH, SOD, MDA, TNF‑α and IL‑6 in the supernatant in each group were statistically significant (P<0.05 or P<0.01). Moreover, there were statistically significant differences in the gray values of p‑p38MAPK/GAPDH and p38MAPK/GAPDH and their ratio p‑p38MAPK/p38MAPK in each group (P<0.05 or P<0.01). Inhibiting the expression of circANRIL in coronary heart disease cases can reduce vascular endothelial injury, oxidative stress and inflammation.

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September-2020
Volume 20 Issue 3

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Spandidos Publications style
Shi P, Ji H, Zhang H, Yang J, Guo R and Wang J: circANRIL reduces vascular endothelial injury, oxidative stress and inflammation in rats with coronary atherosclerosis. Exp Ther Med 20: 2245-2251, 2020
APA
Shi, P., Ji, H., Zhang, H., Yang, J., Guo, R., & Wang, J. (2020). circANRIL reduces vascular endothelial injury, oxidative stress and inflammation in rats with coronary atherosclerosis. Experimental and Therapeutic Medicine, 20, 2245-2251. https://doi.org/10.3892/etm.2020.8956
MLA
Shi, P., Ji, H., Zhang, H., Yang, J., Guo, R., Wang, J."circANRIL reduces vascular endothelial injury, oxidative stress and inflammation in rats with coronary atherosclerosis". Experimental and Therapeutic Medicine 20.3 (2020): 2245-2251.
Chicago
Shi, P., Ji, H., Zhang, H., Yang, J., Guo, R., Wang, J."circANRIL reduces vascular endothelial injury, oxidative stress and inflammation in rats with coronary atherosclerosis". Experimental and Therapeutic Medicine 20, no. 3 (2020): 2245-2251. https://doi.org/10.3892/etm.2020.8956