[Comment] MALDI‑TOF MS‑based direct‑on‑target microdroplet growth assay: Latest developments

Neonakis,Ioannis   K.; Spandidos,Demetrios   A.

doi:10.3892/etm.2020.8976

[Comment] MALDI‑TOF MS‑based direct‑on‑target microdroplet growth assay: Latest developments

Authors:
- Ioannis K. Neonakis
- Demetrios A. Spandidos
View Affiliations

Affiliations: Department of Clinical Microbiology and Microbial Pathogenesis, University Hospital of Heraklion, 71201 Heraklion, Greece, Laboratory of Clinical Virology, School of Medicine, University of Crete, 71003 Heraklion, Greece
Published online on: July 8, 2020 https://doi.org/10.3892/etm.2020.8976
Pages: 2555-2556
Copyright: © Neonakis et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )

Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )

Cited By (CrossRef): 0 citations Loading Articles...

Abstract

The matrix‑assisted laser desorption‑ionization time‑of‑flight mass spectrometry direct‑on‑target microdroplet growth assay for the rapid susceptibility testing and the detection of the underlying antibiotic resistance mechanisms of microbia has been recently introduced. In the present study, we review the latest developments in the field.

View Figures

View References

1	Idelevich EA, Sparbier K, Kostrzewa M and Becker K: Rapid detection of antibiotic resistance by MALDI-TOF mass spectrometry using a novel direct-on-target microdroplet growth assay. Clin Microbiol Infect. 24:738–743. 2018.PubMed/NCBI View Article : Google Scholar
2	Idelevich EA, Storck LM, Sparbier K, Drews O, Kostrzewa M and Becker K: Rapid direct susceptibility testing from positive blood cultures by the matrix-assisted laser desorption ionizationtime of flight mass spectrometry-based direct-on-target microdroplet growth assay. J Clin Microbiol. 56:e00913–e00918. 2018.PubMed/NCBI View Article : Google Scholar
3	Correa-Martínez CL, Idelevich EA, Sparbier K, Kostrzewa M and Becker K: Rapid detection of extended-spectrum β-lactamases (ESBL) and AmpC β-lactamases in Enterobacterales: Development of a screening panel using the MALDI-TOF MS-based direct-on-target microdroplet growth assay. Front Microbiol. 24(13)2019.PubMed/NCBI View Article : Google Scholar
4	Neonakis IK and Spandidos DA: MALDI-TOF mass spectrometry-based direct-on-target microdroplet growth assay: A novel assay for susceptibility testing and beyond. Future Microbiol. 14:729–731. 2019.PubMed/NCBI View Article : Google Scholar
5	Neonakis IK and Spandidos DA: Detection of carbapenemase producers by matrix-assisted laser desorption-ionization time-of-flight mass spectrometry (MALDI-TOF MS). Eur J Clin Microbiol Infect Dis. 38:1795–1801. 2019.PubMed/NCBI View Article : Google Scholar
6	Correa-Martínez CL, Idelevich EA, Sparbier K, Kuczius T, Kostrzewa M and Becker K: Development of a MALDI-TOF MS-based screening panel for accelerated differential detection of carbapenemases in Enterobacterales using the direct-on-target microdroplet growth assay. Sci Rep. 10(4988)2020.PubMed/NCBI View Article : Google Scholar
7	European Committee on Antimicrobial Susceptibility Testing (EUCAST): EUCAST guidelines for detection of resistance mechanisms and specific resistances of clinical and/or epidemiological importance. Version 2.0. https://eucast.org/fileadmin/src/media/PDFs/EUCAST_files/Resistance_mechanisms/EUCAST_detection_of_resistance_mechanisms_170711.pdf. Accessed July, 2017.
8	Nix ID, Idelevich EA, Storck LM, Sparbier K, Drews O, Kostrzewa M and Becker K: Detection of methicillin resistance in Staphylococcus aureus from agar cultures and directly from positive blood cultures using MALDI-TOF mass spectrometry-based direct-on-target microdroplet growth assay. Front Microbiol. 11(232)2020.PubMed/NCBI View Article : Google Scholar
9	Idelevich EA, Kostrzewa M and Becker K: P0440. Differentiation of Streptococcus pneumonia from viridans group streptococci using MALDI-TOF MS-based optochin susceptibility test. In: Proceedings of the 29th ECCMID. ECCMID, Amsterdam, 2019.