Open Access

Bone marrow stromal cells‑derived exosomes target DAB2IP to induce microglial cell autophagy, a new strategy for neural stem cell transplantation in brain injury

  • Authors:
    • Feng‑Ying Yuan
    • Ming‑Xing Zhang
    • Yi‑Hua Shi
    • Mei‑Hui Li
    • Jia‑Yuan Ou
    • Wen‑Fang Bai
    • Ming‑Sheng Zhang
  • View Affiliations

  • Published online on: July 13, 2020     https://doi.org/10.3892/etm.2020.9008
  • Pages: 2752-2764
  • Copyright: © Yuan et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Bone marrow stromal cells (MSCs) are a useful source of stem cells for the treatment of various brain injury diseases due to their abundant supply and fewer ethical problems compared with transplant treatment. However, the clinical application of MSCs is limited due to allograft rejection and immunosuppression in the process of MSCs transplantation. According to previous studies, microglial cell autophagy occurs following co‑culture with MSCs. In the present study, exosomes were obtained from MSCs and subsequently characterized using transmission electron microscopy, atomic force microscopy and dynamic light scattering particle size analysis. The type of microRNAs (miRs) found in the exosomes was then analyzed via gene chip. The results demonstrated that microglial cell autophagy could be induced by exosomes. This mechanism was therefore investigated further via reverse transcription‑quantitative PCR, western blotting and luciferase assays. These results demonstrated that exosomes from MSCs could induce microglial cell autophagy through the miR‑32‑mediated regulation of disabled homolog 2‑interacting protein, thus providing a theoretical basis for the clinical application of miRs in MSCs.

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September-2020
Volume 20 Issue 3

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Yuan FY, Zhang MX, Shi YH, Li MH, Ou JY, Bai WF and Zhang MS: Bone marrow stromal cells‑derived exosomes target DAB2IP to induce microglial cell autophagy, a new strategy for neural stem cell transplantation in brain injury. Exp Ther Med 20: 2752-2764, 2020
APA
Yuan, F., Zhang, M., Shi, Y., Li, M., Ou, J., Bai, W., & Zhang, M. (2020). Bone marrow stromal cells‑derived exosomes target DAB2IP to induce microglial cell autophagy, a new strategy for neural stem cell transplantation in brain injury. Experimental and Therapeutic Medicine, 20, 2752-2764. https://doi.org/10.3892/etm.2020.9008
MLA
Yuan, F., Zhang, M., Shi, Y., Li, M., Ou, J., Bai, W., Zhang, M."Bone marrow stromal cells‑derived exosomes target DAB2IP to induce microglial cell autophagy, a new strategy for neural stem cell transplantation in brain injury". Experimental and Therapeutic Medicine 20.3 (2020): 2752-2764.
Chicago
Yuan, F., Zhang, M., Shi, Y., Li, M., Ou, J., Bai, W., Zhang, M."Bone marrow stromal cells‑derived exosomes target DAB2IP to induce microglial cell autophagy, a new strategy for neural stem cell transplantation in brain injury". Experimental and Therapeutic Medicine 20, no. 3 (2020): 2752-2764. https://doi.org/10.3892/etm.2020.9008