Horseradish peroxidase interacts with the cell wall peptidoglycans on oral bacteria

  • Authors:
    • Hirofumi Mizuno
    • Eiji Takayama
    • Ayano Satoh
    • Takeshi Into
    • Masanori Adachi
    • Daisuke Ekuni
    • Koji Yashiro
    • Masako Mizuno‑Kamiya
    • Motohiko Nagayama
    • Seitaro Saku
    • Takaaki Tomofuji
    • Yutaka Doi
    • Yukitaka Murakami
    • Nobuo Kondoh
    • Manabu Morita
  • View Affiliations

  • Published online on: July 16, 2020     https://doi.org/10.3892/etm.2020.9016
  • Pages: 2822-2827
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Abstract

Salivary peroxidase and myeloperoxidase are known to display antibacterial activity against oral microbes, and previous indications have pointed to the possibility that horseradish peroxidase (HRP) adsorbs onto the membrane of the major oral streptococci, Streptococcus mutans and Streptococcus sanguinis (S. sanguinis). However, the mechanism of interaction between HRP and the bacterial cell wall component is unclear. Dental plaques containing salivary glycoproteins and extracellular microbial products are visualized with ‘dental plaque disclosing agent’, and are controlled within dental therapy. However, current ‘dental plaque disclosing agents’ are difficult to evaluate with just dental plaques, since they stain and disclose not only dental plaques but also pellicle formed with salivary glycoproteins on a tooth surface. In this present study, we have demonstrated that HRP interacted with the cell wall component of the major gram‑positive bacterial peptidoglycan, but not the major cell wall component of gram‑negative bacteria lipopolysaccharide. Furthermore, we observed that the adsorbed HRP labeled with fluorescence was detected on the major oral gram‑positive strains S. sanguinis and Streptococcus salivarius (S. salivarius), but not on a gram‑negative strain, Escherichia coli (E. coli). Furthermore, we have demonstrated that the combination of HRP and chromogenic substrate clearly disclosed the dental plaques and the biofilm developed by S. sanguinis, S. salivarius and the major gram‑postive bacteria Lactobacillus casei on tooth surfaces, and slightly disclosed the biofilm by E. coli. The combination of HRP and chromogenic substrate did not stain either the dental pellicle with the salivary glycoprotein mucin, or naked tooth surfaces. These results have suggested the possibility that the adsorption activity of HRP not only contributes to the evaluation of dental plaque, but that enzymatic activity of HRP may also contribute to improve dental hygiene.

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September-2020
Volume 20 Issue 3

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Mizuno H, Takayama E, Satoh A, Into T, Adachi M, Ekuni D, Yashiro K, Mizuno‑Kamiya M, Nagayama M, Saku S, Saku S, et al: Horseradish peroxidase interacts with the cell wall peptidoglycans on oral bacteria . Exp Ther Med 20: 2822-2827, 2020
APA
Mizuno, H., Takayama, E., Satoh, A., Into, T., Adachi, M., Ekuni, D. ... Morita, M. (2020). Horseradish peroxidase interacts with the cell wall peptidoglycans on oral bacteria . Experimental and Therapeutic Medicine, 20, 2822-2827. https://doi.org/10.3892/etm.2020.9016
MLA
Mizuno, H., Takayama, E., Satoh, A., Into, T., Adachi, M., Ekuni, D., Yashiro, K., Mizuno‑Kamiya, M., Nagayama, M., Saku, S., Tomofuji, T., Doi, Y., Murakami, Y., Kondoh, N., Morita, M."Horseradish peroxidase interacts with the cell wall peptidoglycans on oral bacteria ". Experimental and Therapeutic Medicine 20.3 (2020): 2822-2827.
Chicago
Mizuno, H., Takayama, E., Satoh, A., Into, T., Adachi, M., Ekuni, D., Yashiro, K., Mizuno‑Kamiya, M., Nagayama, M., Saku, S., Tomofuji, T., Doi, Y., Murakami, Y., Kondoh, N., Morita, M."Horseradish peroxidase interacts with the cell wall peptidoglycans on oral bacteria ". Experimental and Therapeutic Medicine 20, no. 3 (2020): 2822-2827. https://doi.org/10.3892/etm.2020.9016