Atherosclerosis prediction by microarray-based DNA methylation analysis

  • Authors:
    • Wei-Da Chen
    • Ting Song
    • Qiu-Hong Cao
    • Rui Li
    • Hua Wang
    • Xiu-Bao Chen
    • Ze-Tao Chen
  • View Affiliations

  • Published online on: July 21, 2020     https://doi.org/10.3892/etm.2020.9025
  • Pages: 2863-2869
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Abstract

Using a series of DNA methylation analysis, pathogenesis was investigated to identify the specific DNA methylation markers for diagnosing atherosclerosis. Firstly, with the chip platform of Illumina Human Methylation 450 BeadChip, a total of 1,458 CpGs, covering 971 differential methylated genes were extracted with stringent filtering criteria. Secondly, hierarchical clustering as a heat map was used to check on the dependability of differential methylated genes. Thirdly, the related GO terms and pathways were enriched by up- and down-methylated genes, respectively, after verifying the capacity of these differential methylated genes to distinguish between atherosclerosis and healthy controls. In total, 971 differential DNA methylated genes were identified (1,458 CpGs). Several important function regions were also identified, including cell adhesion, PI3K-Akt signaling pathway and transcription from RNA polymerase II promoter. This study indicates that patients with atherosclerosis have high levels of DNA methylation, which is promising for early diagnosis and treatment of atherosclerosis.

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September-2020
Volume 20 Issue 3

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Chen W, Song T, Cao Q, Li R, Wang H, Chen X and Chen Z: Atherosclerosis prediction by microarray-based DNA methylation analysis. Exp Ther Med 20: 2863-2869, 2020
APA
Chen, W., Song, T., Cao, Q., Li, R., Wang, H., Chen, X., & Chen, Z. (2020). Atherosclerosis prediction by microarray-based DNA methylation analysis. Experimental and Therapeutic Medicine, 20, 2863-2869. https://doi.org/10.3892/etm.2020.9025
MLA
Chen, W., Song, T., Cao, Q., Li, R., Wang, H., Chen, X., Chen, Z."Atherosclerosis prediction by microarray-based DNA methylation analysis". Experimental and Therapeutic Medicine 20.3 (2020): 2863-2869.
Chicago
Chen, W., Song, T., Cao, Q., Li, R., Wang, H., Chen, X., Chen, Z."Atherosclerosis prediction by microarray-based DNA methylation analysis". Experimental and Therapeutic Medicine 20, no. 3 (2020): 2863-2869. https://doi.org/10.3892/etm.2020.9025