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Article Open Access

miR‑29b enhances the proliferation and migration of bone marrow mesenchymal stem cells in rats with castration‑induced osteoporosis through the PI3K/AKT and TGF‑β/Smad signaling pathways

Retraction in: /10.3892/etm.2022.11644
  • Authors:
    • Yuhui Wang
    • Xiangmin Han
    • Tongxin Zang
    • Ping Kang
    • Wei Jiang
    • Niu Niu
  • View Affiliations / Copyright

    Affiliations: Department of Clinical Laboratory, Affiliated Hospital of Beihua University, Jilin, Jilin 132011, P.R. China, Department of Clinical Laboratory, Jinan Zhangqiu District Hospital of TCM, Jinan, Shandong 250200, P.R. China, Department of Orthopaedics, Qingdao Central Hospital, Qingdao University, Qingdao, Shandong 266042, P.R. China, Department of Rehabilitation, The People's Hospital of Zhangqiu Area, Jinan, Shandong 250200, P.R. China, Department of Cardio‑Thoracic Surgery, The People's Hospital of Zhangqiu Area, Jinan, Shandong 250200, P.R. China, Department of Clinical Laboratory, Qingdao No. 9 People's Hospital, Qingdao, Shandong 266002, P.R. China
    Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 3185-3195
    |
    Published online on: July 24, 2020
       https://doi.org/10.3892/etm.2020.9045
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Abstract

The aim of the present study was to investigate the role of microRNA (miR)-29b in the proliferation and migration of bone marrow mesenchymal stem cells (BMSCs) in rats with castration‑induced osteoporosis and the relevant mechanisms. The gene expression profiling microarray technique was utilized to sequence the BMSCs with overexpressed miR‑29b. The intersection of the potential targets and the genes downregulated in the sequencing were utilized for GO enrichment analysis. Gene set enrichment analysis (GSEA) was employed to analyze the effect of miR‑29b on signaling pathways. Additionally, the effects of miR‑29b overexpression on the phosphatidylinositol 3‑kinase (PI3K)/protein kinase B (AKT) and transforming growth factor‑β (TGF‑β)/Drosophila mothers against decapentaplegic protein (Smad) signaling pathways were detected via RT‑qPCR assay and western blotting. The expression level of miR‑29b was found to be significantly reduced in bone marrow tissues of postmenopausal osteoporosis patients and BMSCs of rats with castration‑induced osteoporosis established via ovariectomy. Based on transcriptome sequencing and bioinformatics software prediction, 76 potential targets of miR‑29b were obtained, which were distinctly enriched in such biological processes as cell proliferation, cell cycle, cell migration and cell adhesion. The results of CCK‑8 and EdU assays showed that overexpression of miR‑29b overtly promoted the proliferation of BMSCs in rats with castration‑induced osteoporosis. Moreover, the Transwell assay results revealed that the overexpression of miR‑29b significantly facilitated the migration of BMSCs in rats with castration‑induced osteoporosis. According to RT‑qPCR assay and western blotting, miR‑29b activated the PI3K/AKT and TGF‑β/Smad signaling pathways. miR‑29b exhibited a clearly lower expression level in the bone marrow tissues of the postmenopausal osteoporosis patients and BMSCs of rats with castration‑induced osteoporosis established via ovariectomy. Overexpression of miR‑29b was able to enhance the proliferation and migration ability of BMSCs in rats with castration‑induced osteoporosis, and such an enhancement may be correlated with the activation of the PI3K/AKT and TGF‑β/Smad signaling pathways.
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Copy and paste a formatted citation
Spandidos Publications style
Wang Y, Han X, Zang T, Kang P, Jiang W and Niu N: miR‑29b enhances the proliferation and migration of bone marrow mesenchymal stem cells in rats with castration‑induced osteoporosis through the PI3K/AKT and TGF‑β/Smad signaling pathways Retraction in /10.3892/etm.2022.11644. Exp Ther Med 20: 3185-3195, 2020.
APA
Wang, Y., Han, X., Zang, T., Kang, P., Jiang, W., & Niu, N. (2020). miR‑29b enhances the proliferation and migration of bone marrow mesenchymal stem cells in rats with castration‑induced osteoporosis through the PI3K/AKT and TGF‑β/Smad signaling pathways Retraction in /10.3892/etm.2022.11644. Experimental and Therapeutic Medicine, 20, 3185-3195. https://doi.org/10.3892/etm.2020.9045
MLA
Wang, Y., Han, X., Zang, T., Kang, P., Jiang, W., Niu, N."miR‑29b enhances the proliferation and migration of bone marrow mesenchymal stem cells in rats with castration‑induced osteoporosis through the PI3K/AKT and TGF‑β/Smad signaling pathways Retraction in /10.3892/etm.2022.11644". Experimental and Therapeutic Medicine 20.4 (2020): 3185-3195.
Chicago
Wang, Y., Han, X., Zang, T., Kang, P., Jiang, W., Niu, N."miR‑29b enhances the proliferation and migration of bone marrow mesenchymal stem cells in rats with castration‑induced osteoporosis through the PI3K/AKT and TGF‑β/Smad signaling pathways Retraction in /10.3892/etm.2022.11644". Experimental and Therapeutic Medicine 20, no. 4 (2020): 3185-3195. https://doi.org/10.3892/etm.2020.9045
Copy and paste a formatted citation
x
Spandidos Publications style
Wang Y, Han X, Zang T, Kang P, Jiang W and Niu N: miR‑29b enhances the proliferation and migration of bone marrow mesenchymal stem cells in rats with castration‑induced osteoporosis through the PI3K/AKT and TGF‑β/Smad signaling pathways Retraction in /10.3892/etm.2022.11644. Exp Ther Med 20: 3185-3195, 2020.
APA
Wang, Y., Han, X., Zang, T., Kang, P., Jiang, W., & Niu, N. (2020). miR‑29b enhances the proliferation and migration of bone marrow mesenchymal stem cells in rats with castration‑induced osteoporosis through the PI3K/AKT and TGF‑β/Smad signaling pathways Retraction in /10.3892/etm.2022.11644. Experimental and Therapeutic Medicine, 20, 3185-3195. https://doi.org/10.3892/etm.2020.9045
MLA
Wang, Y., Han, X., Zang, T., Kang, P., Jiang, W., Niu, N."miR‑29b enhances the proliferation and migration of bone marrow mesenchymal stem cells in rats with castration‑induced osteoporosis through the PI3K/AKT and TGF‑β/Smad signaling pathways Retraction in /10.3892/etm.2022.11644". Experimental and Therapeutic Medicine 20.4 (2020): 3185-3195.
Chicago
Wang, Y., Han, X., Zang, T., Kang, P., Jiang, W., Niu, N."miR‑29b enhances the proliferation and migration of bone marrow mesenchymal stem cells in rats with castration‑induced osteoporosis through the PI3K/AKT and TGF‑β/Smad signaling pathways Retraction in /10.3892/etm.2022.11644". Experimental and Therapeutic Medicine 20, no. 4 (2020): 3185-3195. https://doi.org/10.3892/etm.2020.9045
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