IL‑1β promotes osteogenic differentiation of mouse bone marrow mesenchymal stem cells via the BMP/Smad pathway within a certain concentration range

Wang,Hao; Ni,Zhihao; Yang,Jiazhao; Li,Meng; Liu,Lei; Pan,Xuejie; Xu,Lei; Wang,Xujin; Fang,Shiyuan

doi:10.3892/etm.2020.9065

IL‑1β promotes osteogenic differentiation of mouse bone marrow mesenchymal stem cells via the BMP/Smad pathway within a certain concentration range

Authors:
- Hao Wang
- Zhihao Ni
- Jiazhao Yang
- Meng Li
- Lei Liu
- Xuejie Pan
- Lei Xu
- Xujin Wang
- Shiyuan Fang
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Affiliations: Department of Orthopedics, Huainan First People's Hospital, Anhui University of Science and Technology, Huainan, Anhui 232000, P.R. China, Department of Orthopedics, Hefei First People's Hospital, Anhui Medical University, Hefei, Anhui 230000, P.R. China, Department of Orthopedics, Anhui Provincial Hospital, Anhui Medical University, Hefei, Anhui 230000, P.R. China
Published online on: July 28, 2020 https://doi.org/10.3892/etm.2020.9065
Pages: 3001-3008
Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Inflammatory factors play an important role in the process of fracture healing. The influence of interleukin (IL)‑1β, a key inflammatory factory, on new bone formation has been controversial. The aim of the present study was to investigate whether IL‑1β affects the osteogenic differentiation of mouse bone marrow mesenchymal stem cells (MBMMSCs), and examined its effective concentration range and molecular mechanism of action. MBMMSC proliferation in the presence of IL‑1β was observed using a Cell‑Counting Kit‑8 assay, and the effect of IL‑1β on MBMMSC apoptosis was examined via flow cytometry. Alkaline phosphatase assay, Alizarin Red staining and quantitative assays were performed to evaluate the osteogenic differentiation of MBMMSCs. The expression levels of osteogenic differentiation markers were detected using reverse transcription‑quantitative PCR (RT‑qPCR). It was demonstrated that within a concentration range of 0.01‑1 ng/ml, IL‑1β promoted osteogenic differentiation of MBMMSCs and did not induce apoptosis. Furthermore, RT‑qPCR results indicated that IL‑1β increased osteogenic gene expression within this concentration range. Moreover, Western blotting results identified that the bone morphogenetic protein/Smad (BMP/Smad) signaling pathway was significantly activated by IL‑1β under osteogenic conditions. Therefore, the present results suggested that within a certain concentration range, IL‑1β promoted osteogenic differentiation and function of MBMMSCs via the BMP/Smad signaling pathway.

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