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Diagnostic and prognostic value of the peripheral natural killer cell levels in gastric cancer

  • Authors:
    • Yu‑Yan Chen
    • Ying Feng
    • Qin‑Sheng Mao
    • Peng Ma
    • Jia‑Zhou Liu
    • Wei Lu
    • Yi‑Fei Liu
    • Xi Chen
    • Yi‑Lin Hu
    • Wan‑Jiang Xue
  • View Affiliations / Copyright

    Affiliations: Department of Gastrointestinal Surgery, Affiliated Hospital of Nantong University, Nantong, Jiangsu 226001, P.R. China, Department of Hematology, Affiliated Hospital of Nantong University, Nantong, Jiangsu 226001, P.R. China, Department of Pathology, Affiliated Hospital of Nantong University, Nantong, Jiangsu 226001, P.R. China
    Copyright: © Chen et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 3816-3822
    |
    Published online on: August 5, 2020
       https://doi.org/10.3892/etm.2020.9101
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Abstract

Peripheral blood lymphocyte subsets have been reported to be useful as prognostic and/or diagnostic markers for patients with cancer. However, the clinical value of peripheral blood lymphocyte subsets in gastric cancer (GC) has remained elusive. In the present study, peripheral CD3+, CD4+ and CD8+ T lymphocytes, B cells (CD19+), regulatory T cells (Tregs; CD4+CD25+CD127‑) and natural killer (NK) cells (CD3‑CDl6+CD56+) were detected by flow cytometry in 122 patients with GC, 80 healthy donors (HDs) and 80 patients with gastric ulcer (GU). NK cells (CD56+) were detected by immunohistochemical (IHC) analysis in 20 GC and three GU tissue samples. A receiver‑operating characteristic (ROC) curve was used to determine the threshold of the peripheral NK cell level and survival analysis was performed to assess its prognostic value in patients with GC. The results indicated that the peripheral NK cell proportion in patients with GC (18.77%) was significantly higher than that in the HD (12.19%) and GU (12.74%) groups. IHC analysis suggested that the NK level in GC tumor samples was correlated with that in paired serum samples. ROC curve analysis indicated that the peripheral NK cell level (15.16%) was able to effectively identify patients with GC, a diagnostic sensitivity of 75.41% and a specificity of 77.45% were determined. Multivariate logistic regression analysis revealed that the peripheral NK cell level was independently associated with the T stage and survival analysis demonstrated that high levels of peripheral NK cells were associated with poor prognosis of patients with GC. In conclusion, the peripheral NK cell level may be a diagnostic and prognostic marker for patients with GC.
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Copy and paste a formatted citation
Spandidos Publications style
Chen YY, Feng Y, Mao QS, Ma P, Liu JZ, Lu W, Liu YF, Chen X, Hu YL, Xue WJ, Xue WJ, et al: Diagnostic and prognostic value of the peripheral natural killer cell levels in gastric cancer. Exp Ther Med 20: 3816-3822, 2020.
APA
Chen, Y., Feng, Y., Mao, Q., Ma, P., Liu, J., Lu, W. ... Xue, W. (2020). Diagnostic and prognostic value of the peripheral natural killer cell levels in gastric cancer. Experimental and Therapeutic Medicine, 20, 3816-3822. https://doi.org/10.3892/etm.2020.9101
MLA
Chen, Y., Feng, Y., Mao, Q., Ma, P., Liu, J., Lu, W., Liu, Y., Chen, X., Hu, Y., Xue, W."Diagnostic and prognostic value of the peripheral natural killer cell levels in gastric cancer". Experimental and Therapeutic Medicine 20.4 (2020): 3816-3822.
Chicago
Chen, Y., Feng, Y., Mao, Q., Ma, P., Liu, J., Lu, W., Liu, Y., Chen, X., Hu, Y., Xue, W."Diagnostic and prognostic value of the peripheral natural killer cell levels in gastric cancer". Experimental and Therapeutic Medicine 20, no. 4 (2020): 3816-3822. https://doi.org/10.3892/etm.2020.9101
Copy and paste a formatted citation
x
Spandidos Publications style
Chen YY, Feng Y, Mao QS, Ma P, Liu JZ, Lu W, Liu YF, Chen X, Hu YL, Xue WJ, Xue WJ, et al: Diagnostic and prognostic value of the peripheral natural killer cell levels in gastric cancer. Exp Ther Med 20: 3816-3822, 2020.
APA
Chen, Y., Feng, Y., Mao, Q., Ma, P., Liu, J., Lu, W. ... Xue, W. (2020). Diagnostic and prognostic value of the peripheral natural killer cell levels in gastric cancer. Experimental and Therapeutic Medicine, 20, 3816-3822. https://doi.org/10.3892/etm.2020.9101
MLA
Chen, Y., Feng, Y., Mao, Q., Ma, P., Liu, J., Lu, W., Liu, Y., Chen, X., Hu, Y., Xue, W."Diagnostic and prognostic value of the peripheral natural killer cell levels in gastric cancer". Experimental and Therapeutic Medicine 20.4 (2020): 3816-3822.
Chicago
Chen, Y., Feng, Y., Mao, Q., Ma, P., Liu, J., Lu, W., Liu, Y., Chen, X., Hu, Y., Xue, W."Diagnostic and prognostic value of the peripheral natural killer cell levels in gastric cancer". Experimental and Therapeutic Medicine 20, no. 4 (2020): 3816-3822. https://doi.org/10.3892/etm.2020.9101
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