Open Access

PCR coupled to electrospray ionization mass spectrometry formicrobiological diagnosis and surveillance of ventilator‑associated pneumonia

  • Authors:
    • Dongni Hou
    • Mohan Ju
    • Ying Wang
    • Donghui Zhang
    • Duming Zhu
    • Ming Zhong
    • Chenjun Zhou
    • Yuanlin Song
    • Xunjia Cheng
  • View Affiliations

  • Published online on: August 6, 2020     https://doi.org/10.3892/etm.2020.9103
  • Pages: 3587-3594
  • Copyright: © Hou et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Etiological diagnosis is essential for anti‑infective therapy in patients with ventilator‑associated pneumonia (VAP). The present study aimed to evaluate the capacity of sequential PCR coupled to electrospray ionization mass spectrometry (PCR/ESI‑MS) tests as a rapid diagnostic technique for patients with VAP. A total of 12 patients diagnosed with VAP were enrolled at the intensive care unit in Zhongshan Hospital, Fudan University. Mini‑bronchoalveolar lavage fluid specimens were prospectively collected on VAP 0, 5 and 10 days following the beginning of mechanical ventilation. Routine clinical culture and PCR/ESI‑MS were compared for identification of microorganisms in the specimens. A total of 51 bacterial species were detected by either of the two methods. The positive rates of routine clinical culture and PCR/ESI‑MS were 38.2 and 88.2%, respectively. Out of the 16 specimens positive in routine cultures, 15 were also positive on PCR/ESI‑MS, except for one, from which a mix of three distinct bacterial isolates were reported by culture. Among the 50 bacterial species identified by PCR/ESI‑MS, 15 (35.7%) of the common VAP pathogens were confirmed by paired culture. Furthermore, of the 16 bacterial isolates that were finally confirmed to be responsible for VAP, 14 were identified by a sequential PCR/ESI‑MS test concurrently when the culture results were obtained. PCR/ESI‑MS identified pathogens that may cause VAP in 8 subjects prior to the occurrence of associated clinical manifestations. To conclude, PCR/ESI‑MS was a potential rapid technique for diagnosis of VAP within 6 h. Regular respiratory specimen monitoring using PCR/ESI‑MS provides information for selecting appropriate and adequate antibiotic therapies in ventilated patients.
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October-2020
Volume 20 Issue 4

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Hou D, Ju M, Wang Y, Zhang D, Zhu D, Zhong M, Zhou C, Song Y and Cheng X: PCR coupled to electrospray ionization mass spectrometry formicrobiological diagnosis and surveillance of ventilator‑associated pneumonia. Exp Ther Med 20: 3587-3594, 2020
APA
Hou, D., Ju, M., Wang, Y., Zhang, D., Zhu, D., Zhong, M. ... Cheng, X. (2020). PCR coupled to electrospray ionization mass spectrometry formicrobiological diagnosis and surveillance of ventilator‑associated pneumonia. Experimental and Therapeutic Medicine, 20, 3587-3594. https://doi.org/10.3892/etm.2020.9103
MLA
Hou, D., Ju, M., Wang, Y., Zhang, D., Zhu, D., Zhong, M., Zhou, C., Song, Y., Cheng, X."PCR coupled to electrospray ionization mass spectrometry formicrobiological diagnosis and surveillance of ventilator‑associated pneumonia". Experimental and Therapeutic Medicine 20.4 (2020): 3587-3594.
Chicago
Hou, D., Ju, M., Wang, Y., Zhang, D., Zhu, D., Zhong, M., Zhou, C., Song, Y., Cheng, X."PCR coupled to electrospray ionization mass spectrometry formicrobiological diagnosis and surveillance of ventilator‑associated pneumonia". Experimental and Therapeutic Medicine 20, no. 4 (2020): 3587-3594. https://doi.org/10.3892/etm.2020.9103