Tetramethylpyrazine ameliorates isoflurane‑induced cognitive dysfunction by inhibiting neuroinflammation via miR‑150 in rats
- Authors:
- Published online on: August 7, 2020 https://doi.org/10.3892/etm.2020.9110
- Pages: 3878-3887
Abstract
Introduction
Anesthetic-induced cognitive dysfunction is a frequent complication following major surgery (1), and the typical symptoms include memory deficits and impaired information processing after surgery. Some patients with anesthetic-induced cognitive dysfunction can regress, but an increasing number of cases have been found to involve long-term cognitive dysfunction that leads to a significantly decreased quality of life in patients (2). For example, it is reported that ~30-50% patients suffer from postoperative cognitive dysfunction after cardiac surgery, and only 45% of them can recover to baseline cognitive function by one year after surgery (3). Isoflurane is a generally used anesthetic that has been reported to induce cognitive dysfunction in patients after surgery (4). Previous studies have provided evidence for neuroinflammation as a key event in the pathogenesis of isoflurane-induced cognitive dysfunction (5). For example, increased inflammatory cytokines have been observed to be induced by anesthesia in brain after surgery (6), and isoflurane-induced cognitive dysfunction can be attenuated by suppressing neuroinflammation (7).
Tetramethylpyrazine (TMP) is an alkaloid extracted from the roots of Ligusticum chuanxiong Hort (8), and has important biological actions, including anti-inflammatory, cardioprotective, antioxidant, immune-enhanced and neuroprotective effects (9,10). Moreover, TMP has been reported to exert a neuroprotective role in Parkinson's disease (11) and cerebral ischemia/reperfusion (12). However, whether TMP can also improve the cognitive dysfunction induced by isoflurane remains unknown. Previous studies have revealed that TMP can improve certain diseases by inhibiting inflammatory reactions, especially neuroinflammation. For example, Fu et al (13) found an antidepressant effect of TMP in a chronic unpredictable mild stress mouse model via the suppression of the Toll-like receptor 4 inflammatory signaling pathway. In addition, Wang et al (14) demonstrated that TMP exerts a protective role against traumatic brain injury by attenuating neuroinflammation. Thus, it was hypothesized that TMP may regulate the inflammatory reaction in isoflurane-induced cognitive dysfunction.
MicroRNAs (miRNAs/miRs) are a group of small non-coding RNAs with extensive biological function in various human diseases, such as metabolic homoeostasis (15). Numerous miRNAs have been identified as key molecules in neurodevelopment, such as miR-200a and miR-302c (16). In addition, certain miRNAs exhibit neuroprotective effects by regulating neuroinflammation (17). miR-150 has been suggested to be an inhibitor of neuroinflammation (18), and it can ameliorate neuropathic pain by targeting AKT3, which is a major regulator of the inflammatory response (19). However, to the best of our knowledge, the relationship between miR-150 and anesthetic-induced cognitive dysfunction has rarely been reported.
Previous studies have examined the role of miRNAs in the protective mechanisms of traditional Chinese herbal medicines in various human diseases, such as miR-294(20). To investigate novel approaches to attenuate the impairment in cognition induced by isoflurane, the present study aimed to evaluate the protective role of TMP against isoflurane-induced cognitive dysfunction in rats, and to further identify the underlying mechanism by assessing the role of miR-150 and its effects on neuroinflammation.
Materials and methods
Animals and exposure to isoflurane
In total, 100 male Sprague-Dawley at 7 weeks of age (220-260 g) rats were purchased from the Animal Center of Chinese Academy of Sciences, and were housed in animal facilities with free access to water and food under a standard environment (25±1°C, 60-65% humidity and 12/12 h light/dark cycle). The rats were randomly designed into two groups: Control (n=10) and isoflurane groups (n=90). For isoflurane exposure, the rats were housed in anesthetic chambers containing 2% isoflurane (Sigma-Aldrich; Merck KGaA) mixed with 100% oxygen for 4 h. The rats in the control group were placed in the animal facilities with room air. All the animal experiments were in accordance with the guidelines of the National Institutes of Health and Association for Assessment and Accreditation of Laboratory Animal Care (21), and were approved by the Animal Care and Use Committee of Dongying Hospital of Traditional Chinese Medicine Hospital.
Animal treatment
The rats that received isoflurane exposure were further assigned to eight groups (10 rats/group): Isoflurane + DMSO vehicle, isoflurane + TMP, isoflurane + miR-negative control (miR-NC), isoflurane + miR-150 mimic, isoflurane + miR-150 inhibitor, isoflurane + TMP + miR-NC, isoflurane + TMP + miR-150 inhibitor and isoflurane groups. For DMSO or TMP treatment, the rats were intraperitoneally injected with DMSO (1 ml) or 20 mg/kg TMP (Nanjing Zelang Medical Technology Co., Ltd.) at 24 h after the isoflurane treatment (22). The miR-150 mimic, miR-150 inhibitor and miR-NC (2 nM) were synthesized by Shanghai GenePharma Co., Ltd. and injected into the rats by lateral cerebroventricular injection to regulate the expression of miR-150 in vivo 24 h before isoflurane exposure, Lipofectamine 2000 (Thermo Fisher Scientific, Inc.) was used for transfection according to the manufacturer's instructions.. The sequences for the vectors were as follows: miR-150 mimic, 5'-UCUCCCAACCCUUGUACCAGUG-3'; miR-150 inhibitor, 5'-CACUGGUACAAGGGUUGGGAGA-3'; and miR-NC, 5'-UUCUCCGAACGUGUCACGU-3'. The detailed treatment for the groups is summarized in Table I. After treatment, five rats from each group were subjected to the Morris water maze test to evaluate the cognitive function, and hippocampi were collected from the remaining five rats after sacrifice by cervical dislocation.
Morris water maze performance
This study evaluated the cognitive function of the rats using a Morris water maze test, which was performed using a method previously described (23). Briefly, 48 h post-isoflurane exposure, the rats in each group were subjected to the hidden-platform training for 5 days with 2 trials per day, and the platform was removed on the 6th day. A computerized tracking system (Actimetrics software version 2.6; Actimetrics) was used to monitor the trials, and the performance, including latency to find the hidden platform, distance traveled, time spent in each quadrant and number of platform crossing, of the rats in different groups was recorded and compared. After the test, the rats (weight, 250-300 g) were sacrificed by cervical dislocation under anesthesia with 50 mg/kg pentobarbitone.
ELISA
The expression levels of the pro-inflammatory cytokines IL-1β (cat. no. RLB00), IL-6 (cat. no. R6000B) and TNF-α (cat. no. RTA00) in the hippocampus collected from the rats in each group were detected using the rat ELISA kit (Invitrogen; Thermo Fisher Scientific, Inc.), following the manufacturer's instruction.
RNA extraction and reverse transcription-quantitative PCR (RT-qPCR)
Total RNAs in the hippocampus were extracted using TRIzol® reagent (Invitrogen; Thermo Fisher Scientific, Inc.), which were then used for RT to synthesize cDNA using the PrimeScript RT reagent kit (Takara Bio, Inc.) according to the manufacturer's protocols, the conditions were 37˚C for 15 min and 98˚C for 5 min. Hippocampal expression levels of miR-150 and the mRNA expression of AKT3 were measured using RT-qPCR, which was performed using a SYBR green I Master Mix kit (Invitrogen; Thermo Fisher Scientific, Inc.) on a 7500 Real-Time PCR system (Applied Biosystems; Thermo Fisher Scientific, Inc.). The PCR conditions were as follows: 95˚C for 5 min, followed by 40 cycles of 95˚C for 30 sec, 60˚C for 30 sec, and 72˚C for 40 sec and then a final extension at 72˚C for 5 min. The final expression levels were calculated using the 2-ΔΔCq method (24) and normalized to U6 or GAPDH. Primers used in the current study are as follows: miR-150, forward: 5'-TGCGGTCTCCCAACCCTTG-3' and reverse, 5'-CCAGTGCAGGGTCCGAGGT-3'; AKT3, forward, 5'-TGTGGATTTACCTTATCCCCTCA-3' and reverse, 5'-GTTTGGCTTTGGTCGTTCTGT-3'; U6, forward, 5'-CGCTTCGGCAGCACATATACTAAAATTGGAAC-3' and reverse: 5'-GCTTCACGAATTTGCGTGTCATCCTTGC-3'; GAPDH, forward, 5'-AGAAGGCTGGGGCTCATTTG-3' and reverse, 5'-AGGGGCCATCCACAGTCTTC-3'.
Cell culture
Human neuroblastoma SH-SY5Y cells (cat. no. ATCC®CRL-2266™) were purchased from the American Type Culture Collection. Cells were cultured in DMEM (Gibco; Thermo Fisher Scientific, Inc.) containing 10% FBS (Gibco; Thermo Fisher Scientific, Inc.) and 1% penicillin/streptomycin (Gibco; Thermo Fisher Scientific, Inc.) at 37˚C in a humidified incubator with 5% CO2.
Dual-luciferase reporter assay
The potential targets of miR-150 were predicted using TargetScan 7.2 (http://www.targetscan.org/vert_72/), which are presented in Table II, and the 3'-untranslated region (UTR) of AKT3 had the complementary sequence of miR-150. To assess their interaction, a dual-luciferase reporter assay was conducted. The wild-type (WT) and mutant type (MUT) 3'-UTR of AKT3 were cloned into pGL3 reporter vector (50 ng; Promega Corporation) (Sangon Biotech Co., Ltd.) and then were respectively co-transfected into SH-SY5Y cells with miR-150 mimic, miR-150 inhibitor or miR-NC (200 ng) using Lipofectamine® 2000 (Invitrogen; Thermo Fisher Scientific, Inc.), following the manufacturer's instruction. After cell transfection for 48 h, the relative luciferase activity was measured using the Promega Dual-luciferase Reporter Assay kit (Promega Corporation). Renilla luciferase activity was used as the internal control.
Table IIList of the predicted target genes of miR-150 commonly predicted in TargetScan 7.2 database. |
Statistical analysis
Data are presented as the mean ± SD, and were analyzed using SPSS 21.0 (IBM Corp.) and GraphPad Prism 7.0 software (GraphPad Software, Inc.). The differences between groups were assessed using Student's t-test or one-way ANOVA, followed by Tukey's or Bonferroni post hoc test. Moreover, behavioral data were analyzed using a mixed ANOVA and a Bonferroni post hoc test. P<0.05 was considered to indicate a statistically significant difference. Each experiment was repeated at least three times.
Results
Neuroprotective effects of TMP on isoflurane-induced cognitive dysfunction
A Morris water maze test was performed to evaluate the effects of isoflurane and TMP treatment on the cognitive function in the rats. In rats exposed to isoflurane, the latency to find the hidden platform and the distance travelled during the test were significantly increased compared with the control group (all P<0.05; Fig. 1A and B), indicating an impairment in the spatial learning ability of the rats. After removing the hidden platform, it was found that the rats exposed to isoflurane spent less time in target quadrant and crossed through the original platform location fewer times compared with the control group (all P<0.05; Fig. 1C and D), suggesting that the memory ability of the rats was impaired. Moreover, the injection of TMP significantly rescued the isoflurane-induced cognitive dysfunction, as demonstrated by the improved spatial learning and memory indicators in the Morris water maze test (all P<0.05; Fig. 1).
Anti-inflammatory effect of TMP in rats exposed to isoflurane
The impairment in cognitive function induced by isoflurane is accompanied by enhanced inflammatory responses (1). The hippocampal levels of the pro-inflammatory cytokines IL-1β, IL-6 and TNF-α were all increased by exposure to isoflurane (all P<0.05; Fig. 2). However, in the rats that received TMP treatment, the isoflurane-induced increases in IL-1β, IL-6 and TNF-α levels were significantly reversed (all P<0.05; Fig. 2).
Dysregulation of miR-150 in rats under isoflurane anesthesia and TMP treatment
The aberrant expression of miR-150 has been identified in neuropathic pain (19). The results demonstrated that the expression of miR-150 in the hippocampus was significantly decreased in the rats exposed to isoflurane compared with control group (P<0.05; Fig. 3), while this effect was partially abrogated by TMP treatment (P<0.05; Fig. 3).
Effects of miR-150 on isoflurane-induced cognitive dysfunction and neuroinflammation
Based on the decreased expression of miR-150 in rats exposed to isoflurane, the effects of miR-150 on the impaired cognitive function and neuroinflammation induced by isoflurane were further investigated. The expression of miR-150 was upregulated by the miR-150 mimic but was downregulated by the miR-150 inhibitor in hippocampal tissues in vivo (both P<0.05; Fig. 4A). It was found that isoflurane-induced spatial learning and memory impairments were all reversed by the overexpression of miR-150, but were aggravated by the knockdown of miR-150 (all P<0.05; Fig. 4B-E). Moreover, the hippocampal concentrations of IL-1β, IL-6 and TNF-α were ameliorated by the upregulation of miR-150, but the opposite results were obtained in the rats with miR-150 knockdown (all P<0.05; Fig. 4F).
TMP exerts neuroprotective effects by regulating miR-150 in rats exposed to isoflurane
Considering the regulatory activity of TMP on the expression of miR-150 in the isoflurane-treated rats, the role of miR-150 in the neuroprotective effect of TMP in rats was evaluated. In the rats exposed to isoflurane, the increased expression of miR-150 induced by TMP was successfully inhibited by the miR-150 inhibitor (P<0.05; Fig. 5A). Furthermore, the results obtained from the Morris water maze test indicated that the neuroprotective effects of TMP on the cognitive function in the rats exposed to isoflurane were significantly attenuated by the knockdown of miR-150 (all P<0.05; Fig. 5B-E). With regards to neuroinflammation, the decreased hippocampal levels of IL-1β, IL-6 and TNF-α induced by TMP were all upregulated by the miR-150 inhibitor (all P<0.05; Fig. 5F).
AKT3 serves as a target gene of miR-150
miR-150 mimic transfection significantly increased the expression of miR-150 in SH-SY5Y cells, but miR-150 inhibitor transfection had an opposite effect (all P<0.05; Fig. 6A). A complementary sequence of miR-150 was found in the 3'-UTR of AKT3 (Fig. 6B). The luciferase analysis results demonstrated that the overexpression of miR-150 could inhibit the relative luciferase activity, but the knockdown of miR-150 could promote the luciferase activity in the WT group (all P<0.05; Fig. 6C). However, no significant changes were identified in the luciferase activity in MUT groups (all P>0.05).
The relative mRNA expression of AKT3 in the hippocampus collected from the experimental rats was measured. It was demonstrated that isoflurane exposure led to increased expression of AKT3, while TMP treatment could inhibit this increase in AKT3 expression (all P<0.05; Fig. 6D). Furthermore, via the in vivo regulation of miR-150, the mRNA expression of AKT3 in the isoflurane-treated rats was inhibited by the overexpression of miR-150, but was enhanced by the knockdown of miR-150 compared with the isoflurane group (all P<0.05; Fig. 6D). In the isoflurane-treated rats that received both TMP and miR-150 inhibitor administration, the TMP-induced decrease in AKT3 expression was reversed by the knockdown of miR-150 (P<0.05; Fig. 6D).
Discussion
Anesthetic-induced cognitive dysfunction remains a challenging health problem for doctors after major surgery (25). The present study focused on the protective effects of the traditional Chinese herbal medicine TMP in rats exposed to isoflurane and evaluated the underlying mechanisms. The current results suggested that the TMP treatment in rats ameliorated the impairment in cognitive function induced by isoflurane exposure, and that anesthetic-induced neuroinflammation was also attenuated by TMP. In addition, it was found that the hippocampal expression of miR-150 was suppressed by isoflurane exposure, but was enhanced after TMP treatment. The overexpression of miR-150 in rats led to improved cognitive function and neuroinflammation, while the knockdown of miR-150 abrogated the protective effects of TMP against isoflurane-induced cognitive dysfunction and neuroinflammation.
Previous studies have revealed the clinical potential of traditional Chinese herbal medicines in various human diseases, such as hepatitis and neurodegenerative disease (26). Some active components of Chinese medicine can exert significantly protective effects for the treatment of cognitive function impairment induced by anesthetics. For example, Chen et al (1) provided evidence for apigenin as a medicine that attenuates cognitive dysfunction induced by isoflurane in aged rats. Moreover, Zhang et al (27) demonstrated the neuroprotective role of ginsenoside Rg1 in rats exposed to isoflurane, while Chen et al (28) revealed that vitexin exerts a protective effect against neurotoxicity induced by isoflurane. The neuroprotective role of salidroside has also been reported in rats expose to isoflurane (29). These aforementioned studies provide evidence for the important roles of traditional Chinese medicine in neuroprotective effects in rats exposed to isoflurane.
TMP is a widely used and analyzed traditional Chinese medicine in human diseases, such as liver fibrosis (30) and prostate cancer (31). The effects of TMP on the nervous system have been reported in oxygen-glucose deprivation-induced mortality (8) and ischemic stroke (32). However, whether TMP is involved in the neuroregulation in rats exposed to isoflurane remains elusive. In the current study, a Morris water maze test was performed to evaluate the effect of TMP on the spatial learning and memory abilities of rats. It was demonstrated that the impaired cognitive function induced by isoflurane in rats was significantly ameliorated by TMP treatment. Thus, it was concluded that TMP exerts a protective effect against isoflurane-induced cognitive dysfunction.
Neuroinflammation is a key event during impairments of learning and memory in rats exposed to anesthetics (33). The neuroprotective effects of apigenin (1) and ginsenoside Rb1(34) in rats exposed to isoflurane are achieved by attenuating neuroinflammation. TMP has been shown to be closely associated with inflammation, and its anti-inflammatory effect has been well described in vivo (35). The present results also provide evidence that TMP exerts an anti-neuroinflammatory role in rats exposed to isoflurane, and suggested that TMP may ameliorate isoflurane-induced cognitive dysfunction by attenuating neuroinflammation. However, the changes in systemic inflammatory responses were not analyzed, which was one of the limitations of the current study. Despite the presence of a blood-brain barrier, it has been reported that the circulating immune system is also closely associated with neuroinflammation (36). Thus, further studies should investigate the effects of TMP on systemic inflammatory responses to further identify the regulatory mechanisms.
Previous studies have investigated the role of miRNAs in the mechanisms involved in the protective effects of traditional Chinese medicine. For instance, Liu et al (37) revealed that curcumin can enhance sensitivity to radiation in prostate cancer cells via the regulation of miR-143. Moreover, Fan et al (38) demonstrated that emodin protects against hyperglycemia-induced injury in PC-12 cells via the upregulation of miR-9. TMP has been reported to alleviate spinal cord injury via the regulation of miR-214-3p (39) and relieve hypoxia-caused damage via the downregulation of miR-449a (40). However, the number of functional miRNAs involved in the development of isoflurane-induced cognitive dysfunction remains unknown.
miR-150 has been reported to exert protective effects in neuropathic pain by suppressing neuroinflammation (19). Moreover, aberrant expression of miR-150 has been observed in multiple sclerosis after treatment with curcumin (41). In the present study, it was found that the hippocampal expression of miR-150 was significantly decreased in rats exposed to isoflurane, and that this inhibitory effect was relieved by treatment with TMP, suggesting the potential role of miR-150 in the development of isoflurane-induced injury and in the biological function of TMP. Further experiments were performed to verified this conclusion, the results of which indicated that the overexpression of miR-150 in rats improved the impairment in cognitive function and inflammatory reaction induced by isoflurane exposure. Furthermore, the knockdown of miR-150 abrogated the protective effects of TMP on spatial learning and memory abilities, as well as neuroinflammation in rats. Collectively, these results suggested that miR-150 may be a mediator of the neuroprotective effects of TMP via neuroinflammation in rats exposed to isoflurane.
miRNAs are involved in the development of various human disease by regulating their target genes (42). In the present study, a number of candidate target genes were predicted using TargetScan for miR-150, and AKT3 was selected based on previous studies demonstrating its crucial role in neurological function (43,44). AKT3 is a major isoform of the AKT family of serine/threonine protein kinases, which is predominant in the brain (45). The dysregulation of AKT3 has been widely reported to be involved in the regulation of learning and memory deficits in several diseases (43,44). Additionally, AKT3 has been shown to be a target gene of miR-150(19), which is one of the major molecules of the PI3K/AKT signaling pathway. In the present study, it was demonstrated that AKT3 was a direct target of miR-150, and the results indicated the negatively regulatory effect of miR-150 on the expression of AKT3 in rats exposed to isoflurane and administrated with TMP. It was also found that inhibited AKT3 expression induced by TMP could be rescued by the knockdown of miR-150, which suggested that miR-150 may mediate the neuroprotective effect of TMP in isoflurane-treated rats by targeting AKT3. PI3K/AKT signaling is considered an important pathway in regulating neuroinflammation (46). Yang et al (47) revealed that TMP can ameliorate the injury induced by acute myocardial ischemia and attenuated the inflammatory response by regulating the PI3K/AKT signaling pathway. These previous studies combined with the current findings suggest that TMP may protect isoflurane-induced cognitive dysfunction via the miR-150/AKT signaling, and this conclusion warrants further investigation.
Alongside AKT3, other targets of miR-150 have previously been reported, such as glioma-associated oncogene homolog 1 (Gli1) (48) and hypoxia inducible factor-1α (HIF-1α) (49). For instance, Peng et al (50) found that Gli1 was involved in the cerebral ischemia/reperfusion injury after isoflurane post-conditioning. Moreover, HIF-1α has been reported to serve an important role in the blood-brain barrier disruption induced by isoflurane (51), and it could mediate the biological function of TMP in the improvement of tibial dyschodroplasia (52) and endothelial dysfunction (53). However, whether these aforementioned genes also participate the neuroprotective effects of TMP mediated by miR-150 remains unknown, and future studies should focus on the potential targets and signaling to further confirm the mechanisms underlying the functional role of TMP and miR-150 in isoflurane-induced cognitive dysfunction.
In conclusion, the present study provided evidence that TMP and miR-150 exert protective roles against the cognitive dysfunction induced by isoflurane in rats. TMP ameliorates the isoflurane-induced impairments in learning and memory via the attenuation of neuroinflammation by promoting the miR-150/AKT pathway. Moreover, miR-150 may serve as a potential therapeutic target for the alleviation of isoflurane-induced cognitive dysfunction.
Acknowledgements
Not applicable.
Funding
No funding was received.
Availability of data and materials
The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request.
Authors' contributions
HC and ZX made substantial contributions to conception and design, acquisition of data, analysis and interpretation of data. HC, ZX and CQ were involved in animal experiments, drafting the manuscript and revising it. All authors read and approved the final manuscript
Ethics approval and consent to participate
All the animal experiments were in accordance with the guidelines of the National Institutes of Health and Association for Assessment and Accreditation of Laboratory Animal Care, and were approved by the Animal Care and Use Committee of Dongying Hospital of Traditional Chinese Medicine Hospital.
Patient consent for publication
Not applicable.
Competing interests
The authors declare that they have no competing interests.
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