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Klebsiella pneumoniae infection inhibits autophagy by alveolar type II epithelial cells

  • Authors:
    • Zhiyun Shi
    • Gang Li
    • Lin Zhang
    • Miao Ma
    • Wei Jia
  • View Affiliations / Copyright

    Affiliations: Medical Experimental Center, General Hospital of Ningxia Medical University, Yinchuan, Ningxia 750004, P.R. China, Department of Laboratory, Institute of Clinical Medicine, Ningxia Medical University, Yinchuan, Ningxia 750004, P.R. China
    Copyright: © Shi et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 3703-3708
    |
    Published online on: August 19, 2020
       https://doi.org/10.3892/etm.2020.9123
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Abstract

To investigate the molecular mechanism underlying the interaction between autophagosomes of alveolar type II epithelial (A549) cells and Klebsiella pneumoniae, an in vitro model of K. pneumoniae‑infected A549 cells was established. Western blot analysis and immunofluorescence staining were used to detect the distribution of microtubule‑associated protein 1A/1B‑light chain 3 (LC3) and the expression of the LC3‑phosphatidylethanolamine conjugate (LC3‑II). K. pneumoniae‑infected A549 cells were treated with different concentrations of an autophagy inhibitor or promoter for different time periods to assess the level of autophagy. Western blot analysis and immunofluorescence staining showed that K. pneumoniae could induce autophagy by A549 cells. With an increase in bacterial concentration and time of infection, autophagy gradually increased. The autophagy inhibitor significantly downregulated, while the promoter upregulated, expression of the autophagy‑related protein LC3‑II. Autophagy plays an important role in the resistance of alveolar type II epithelial (A549) cells to K. pneumoniae infection.
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Copy and paste a formatted citation
Spandidos Publications style
Shi Z, Li G, Zhang L, Ma M and Jia W: Klebsiella pneumoniae infection inhibits autophagy by alveolar type II epithelial cells. Exp Ther Med 20: 3703-3708, 2020.
APA
Shi, Z., Li, G., Zhang, L., Ma, M., & Jia, W. (2020). Klebsiella pneumoniae infection inhibits autophagy by alveolar type II epithelial cells. Experimental and Therapeutic Medicine, 20, 3703-3708. https://doi.org/10.3892/etm.2020.9123
MLA
Shi, Z., Li, G., Zhang, L., Ma, M., Jia, W."Klebsiella pneumoniae infection inhibits autophagy by alveolar type II epithelial cells". Experimental and Therapeutic Medicine 20.4 (2020): 3703-3708.
Chicago
Shi, Z., Li, G., Zhang, L., Ma, M., Jia, W."Klebsiella pneumoniae infection inhibits autophagy by alveolar type II epithelial cells". Experimental and Therapeutic Medicine 20, no. 4 (2020): 3703-3708. https://doi.org/10.3892/etm.2020.9123
Copy and paste a formatted citation
x
Spandidos Publications style
Shi Z, Li G, Zhang L, Ma M and Jia W: Klebsiella pneumoniae infection inhibits autophagy by alveolar type II epithelial cells. Exp Ther Med 20: 3703-3708, 2020.
APA
Shi, Z., Li, G., Zhang, L., Ma, M., & Jia, W. (2020). Klebsiella pneumoniae infection inhibits autophagy by alveolar type II epithelial cells. Experimental and Therapeutic Medicine, 20, 3703-3708. https://doi.org/10.3892/etm.2020.9123
MLA
Shi, Z., Li, G., Zhang, L., Ma, M., Jia, W."Klebsiella pneumoniae infection inhibits autophagy by alveolar type II epithelial cells". Experimental and Therapeutic Medicine 20.4 (2020): 3703-3708.
Chicago
Shi, Z., Li, G., Zhang, L., Ma, M., Jia, W."Klebsiella pneumoniae infection inhibits autophagy by alveolar type II epithelial cells". Experimental and Therapeutic Medicine 20, no. 4 (2020): 3703-3708. https://doi.org/10.3892/etm.2020.9123
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