Klebsiella pneumoniae infection inhibits autophagy by alveolar type II epithelial cells

Shi,Zhiyun; Li,Gang; Zhang,Lin; Ma,Miao; Jia,Wei

doi:10.3892/etm.2020.9123

Klebsiella pneumoniae infection inhibits autophagy by alveolar type II epithelial cells

Authors:
- Zhiyun Shi
- Gang Li
- Lin Zhang
- Miao Ma
- Wei Jia
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Affiliations: Medical Experimental Center, General Hospital of Ningxia Medical University, Yinchuan, Ningxia 750004, P.R. China, Department of Laboratory, Institute of Clinical Medicine, Ningxia Medical University, Yinchuan, Ningxia 750004, P.R. China
Published online on: August 19, 2020 https://doi.org/10.3892/etm.2020.9123
Pages: 3703-3708
Copyright: © Shi et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

To investigate the molecular mechanism underlying the interaction between autophagosomes of alveolar type II epithelial (A549) cells and Klebsiella pneumoniae, an in vitro model of K. pneumoniae‑infected A549 cells was established. Western blot analysis and immunofluorescence staining were used to detect the distribution of microtubule‑associated protein 1A/1B‑light chain 3 (LC3) and the expression of the LC3‑phosphatidylethanolamine conjugate (LC3‑II). K. pneumoniae‑infected A549 cells were treated with different concentrations of an autophagy inhibitor or promoter for different time periods to assess the level of autophagy. Western blot analysis and immunofluorescence staining showed that K. pneumoniae could induce autophagy by A549 cells. With an increase in bacterial concentration and time of infection, autophagy gradually increased. The autophagy inhibitor significantly downregulated, while the promoter upregulated, expression of the autophagy‑related protein LC3‑II. Autophagy plays an important role in the resistance of alveolar type II epithelial (A549) cells to K. pneumoniae infection.

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