Open Access

Total ginsenosides induce autophagic cell death in cervical cancer cells accompanied by downregulation of bone marrow stromal antigen‑2

  • Authors:
    • Shuai Bian
    • Yue Zhao
    • Fangyu Li
    • Shuyan Lu
    • Ziyan He
    • Siming Wang
    • Xueyuan Bai
    • Daqing Zhao
    • Meichen Liu
    • Jiawen Wang
  • View Affiliations

  • Published online on: April 22, 2021     https://doi.org/10.3892/etm.2021.10099
  • Article Number: 667
  • Copyright: © Bian et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Ginsenosides are important active components in Panax ginseng. In the present study, total ginsenosides (TGNs) were demonstrated to enhance autophagy by promoting acidic vacuole organelle formation, recruitment of enhanced green fluorescent protein‑microtubule‑associated protein light chain 3 and expression of autophagy‑related factors in cervical cancer cell lines. TGN markedly increased the expression of p62 at the transcriptional level, but decreased p62 protein expression in the presence of actinomycin D. The autophagic regulatory effect was reversible. TGN (≤120 µg/ml) did not affect the proliferation of cervical cancer cells under normal culture conditions, but markedly inhibited the growth of serum‑deprived cells. Treatment with an inhibitor of autophagy (3‑methyladenine) impaired TGN‑induced cell death. This suggested that TGN caused autophagic cell death. In addition, western blot analysis demonstrated that the protein level of bone marrow stromal antigen‑2 (BST‑2) was downregulated by TGN. Upregulation of BST‑2 reduced cell death. The results of the combined actions of various monomeric ginsenosides in TGN provide the molecular basis to develop TGN as a promising candidate for cancer therapy.
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July-2021
Volume 22 Issue 1

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Bian S, Zhao Y, Li F, Lu S, He Z, Wang S, Bai X, Zhao D, Liu M, Wang J, Wang J, et al: Total ginsenosides induce autophagic cell death in cervical cancer cells accompanied by downregulation of bone marrow stromal antigen‑2. Exp Ther Med 22: 667, 2021
APA
Bian, S., Zhao, Y., Li, F., Lu, S., He, Z., Wang, S. ... Wang, J. (2021). Total ginsenosides induce autophagic cell death in cervical cancer cells accompanied by downregulation of bone marrow stromal antigen‑2. Experimental and Therapeutic Medicine, 22, 667. https://doi.org/10.3892/etm.2021.10099
MLA
Bian, S., Zhao, Y., Li, F., Lu, S., He, Z., Wang, S., Bai, X., Zhao, D., Liu, M., Wang, J."Total ginsenosides induce autophagic cell death in cervical cancer cells accompanied by downregulation of bone marrow stromal antigen‑2". Experimental and Therapeutic Medicine 22.1 (2021): 667.
Chicago
Bian, S., Zhao, Y., Li, F., Lu, S., He, Z., Wang, S., Bai, X., Zhao, D., Liu, M., Wang, J."Total ginsenosides induce autophagic cell death in cervical cancer cells accompanied by downregulation of bone marrow stromal antigen‑2". Experimental and Therapeutic Medicine 22, no. 1 (2021): 667. https://doi.org/10.3892/etm.2021.10099