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Skeletal microenvironment system utilising bovine bone scaffold co‑cultured with human osteoblasts and osteoclast‑like cells

  • Authors:
    • James Jam Jolly
    • Nur Farhana Mohd Fozi
    • Kok-Yong Chin
    • Sok Kuan Wong
    • Kien Hui Chua
    • Ekram Alias
    • Nur Sabariah Adnan
    • Soelaiman Ima‑Nirwana
  • View Affiliations / Copyright

    Affiliations: Department of Pharmacology, Faculty of Medicine, Pusat Perubatan Universiti Kebangsaan Malaysia, Cheras, Wilayah Persekutuan Kuala Lumpur 56000, Malaysia, Department of Physiology, Faculty of Medicine, Pusat Perubatan Universiti Kebangsaan Malaysia, Cheras, Wilayah Persekutuan Kuala Lumpur 56000, Malaysia
    Copyright: © Jolly et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 680
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    Published online on: April 25, 2021
       https://doi.org/10.3892/etm.2021.10112
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Abstract

A three‑dimensional ex vivo bone cell culture system mimicking the skeletal system is useful for bone tissue engineering and as drug discovery platforms. The present study aimed to establish a three‑dimensional skeletal culture system using native bovine bone scaffolds and human bone cells. Bovine bone scaffolds were cultured with human foetal osteoblasts 1.19 and human peripheral blood mononuclear cells for 21 days under standard culture conditions. The following groups were established: Decalcified unseeded bone scaffold (DUBS) as baseline control, decalcified seeded bone scaffold (DSBS) to mimic osteoporosis condition and undecalcified seeded bone scaffold to mimic normal condition. The scaffold's porosity and cell attachment on the scaffolds were determined using scanning electron microscopy. Histological evaluation was used to examine changes in trabecular bone structure. Dual‑energy X‑ray absorptiometry analysis was performed to determine the bone mineral density (BMD) and bone mineral content (BMC) of the scaffolds. A compression test was performed to examine the total biomechanical strength of the scaffolds. The trabecular thickness and number increased, while the trabecular separationwas reduced slightly in DSBS than in DUBS (P>0.05). The BMD and BMC increased significantly (P<0.05), while the compressive strength only increased slightly in DSBS than in DUBS (P>0.05). In conclusion, the ex vivo skeletal microenvironment comprising native bovine bone scaffolds seeded with bone cells is structurally, functionally and mechanically comparable with natural bone. This system may be used as a platform to understand bone physiology and screen for potential drug candidates.
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1 

Zahedi E, Ansari S, Wu BM, Bencharit S and Moshaverinia A: Hydrogels in craniofacial tissue engineering. In: Biomaterials for Oral and Dental Tissue Engineering. Elsevier, pp47-64, 2018.

2 

Jin H-H, Kim D-H, Kim T-W, Shin KK, Jung JS, Park HC and Yoon SY: In vivo evaluation of porous hydroxyapatite/chitosan-alginate composite scaffolds for bone tissue engineering. Int J Biol Macromol. 51:1079–1085. 2012.PubMed/NCBI View Article : Google Scholar

3 

Venkatesan J, Bhatnagar I, Manivasagan P, Kang K-H and Kim S-K: Alginate composites for bone tissue engineering: A review. Int J Biol Macromol. 72:269–281. 2015.PubMed/NCBI View Article : Google Scholar

4 

Karageorgiou V and Kaplan D: Porosity of 3D biomaterial scaffolds and osteogenesis. Biomaterials. 26:5474–5491. 2005.PubMed/NCBI View Article : Google Scholar

5 

LeGeros RZ: Properties of osteoconductive biomaterials: Calcium phosphates. Clin Orthop Relat Res. 395:81–98. 2002.PubMed/NCBI View Article : Google Scholar

6 

Wang H, Li Y, Zuo Y, Li J, Ma S and Cheng L: Biocompatibility and osteogenesis of biomimetic nano-hydroxyapatite/polyamide composite scaffolds for bone tissue engineering. Biomaterials. 28:3338–3348. 2007.PubMed/NCBI View Article : Google Scholar

7 

Huh J, Lee J, Kim W, Yeo M and Kim G: Preparation and characterization of gelatin/α-TCP/SF biocomposite scaffold for bone tissue regeneration. Int J Biol Macromol. 110:488–496. 2018.PubMed/NCBI View Article : Google Scholar

8 

Murphy CM, Haugh MG and O'Brien FJ: The effect of mean pore size on cell attachment, proliferation and migration in collagen-glycosaminoglycan scaffolds for bone tissue engineering. Biomaterials. 31:461–466. 2010.PubMed/NCBI View Article : Google Scholar

9 

Vozzi G, Corallo C, Carta S, Fortina M, Gattazzo F, Galletti M and Giordano N: Collagen-gelatin-genipin-hydroxyapatite composite scaffolds colonized by human primary osteoblasts are suitable for bone tissue engineering applications: In vitro evidences. J Biomed Mater Res A. 102:1415–1421. 2014.PubMed/NCBI View Article : Google Scholar

10 

Hofmann S, Hagenmüller H, Koch AM, Müller R, Vunjak-Novakovic G, Kaplan DL, Merkle HP and Meinel L: Control of in vitro tissue-engineered bone-like structures using human mesenchymal stem cells and porous silk scaffolds. Biomaterials. 28:1152–1162. 2007.PubMed/NCBI View Article : Google Scholar

11 

Nam YS, Yoon JJ and Park TG: A novel fabrication method of macroporous biodegradable polymer scaffolds using gas foaming salt as a porogen additive. J Biomed Mater Res. 53:1–7. 2000.PubMed/NCBI View Article : Google Scholar

12 

Laurencin C, Lu H and Khan Y: Processing of polymer scaffolds: Polymer-ceramic composite foams. Tissue Eng Part C Methods. 705–714. 2002.PubMed/NCBI View Article : Google Scholar

13 

Lloyd AW: Interfacial bioengineering to enhance surface biocompatibility. Med Device Technol. 13:18–21. 2002.PubMed/NCBI

14 

Velasco MA, Narváez-Tovar CA and Garzón-Alvarado DA: Design, materials, and mechanobiology of biodegradable scaffolds for bone tissue engineering. Biomed Res Int. 2015(729076)2015.PubMed/NCBI View Article : Google Scholar

15 

Ma PX and Zhang R: Microtubular architecture of biodegradable polymer scaffolds. J Biomed Mater Res. 56:469–477. 2001.PubMed/NCBI View Article : Google Scholar

16 

Levenberg S, Huang NF, Lavik E, Rogers AB, Itskovitz-Eldor J and Langer R: Differentiation of human embryonic stem cells on three-dimensional polymer scaffolds. Proc Natl Acad Sci USA. 100:12741–12746. 2003.PubMed/NCBI View Article : Google Scholar

17 

Rezwan K, Chen QZ, Blaker JJ and Boccaccini AR: Biodegradable and bioactive porous polymer/inorganic composite scaffolds for bone tissue engineering. Biomaterials. 27:3413–3431. 2006.PubMed/NCBI View Article : Google Scholar

18 

Guda T, Appleford M, Oh S and Ong JL: A cellular perspective to bioceramic scaffolds for bone tissue engineering: The state of the art. Curr Top Med Chem. 8:290–299. 2008.PubMed/NCBI View Article : Google Scholar

19 

Pilliar RM, Filiaggi MJ, Wells JD, Grynpas MD and Kandel RA: Porous calcium polyphosphate scaffolds for bone substitute applications - in vitro characterization. Biomaterials. 22:963–972. 2001.PubMed/NCBI View Article : Google Scholar

20 

Rodrigues CV, Serricella P, Linhares AB, Guerdes RM, Borojevic R, Rossi MA, Duarte ME and Farina M: Characterization of a bovine collagen-hydroxyapatite composite scaffold for bone tissue engineering. Biomaterials. 24:4987–4997. 2003.PubMed/NCBI View Article : Google Scholar

21 

Zhao H, Ma L, Gao C and Shen J: Fabrication and properties of mineralized collagen-chitosan/hydroxyapatite scaffolds. Polym Adv Technol. 19:1590–1596. 2008.

22 

Schönmeyr BH, Wong AK, Li S, Gewalli F, Cordiero PG and Mehrara BJ: Treatment of hydroxyapatite scaffolds with fibronectin and fetal calf serum increases osteoblast adhesion and proliferation in vitro. Plast Reconstr Surg. 121:751–762. 2008.PubMed/NCBI View Article : Google Scholar

23 

Polo-Corrales L, Latorre-Esteves M and Ramirez-Vick JE: Scaffold design for bone regeneration. J Nanosci Nanotechnol. 14:15–56. 2014.PubMed/NCBI View Article : Google Scholar

24 

Silva GAB, Bertassoli BM, Sousa CA, Albergaria JD, de Paula RS and Jorge EC: Effects of strontium ranelate treatment on osteoblasts cultivated onto scaffolds of trabeculae bovine bone. J Bone Miner Metab. 36:73–86. 2018.PubMed/NCBI View Article : Google Scholar

25 

Shahabipour F, Mahdavi-Shahri N, Matin MM, Tavassoli A and Zebarjad SM: Scaffolds derived from cancellous bovine bone support mesenchymal stem cells' maintenance and growth. In Vitro Cell Dev Biol Anim. 49:440–448. 2013.PubMed/NCBI View Article : Google Scholar

26 

Mastrangelo F, Quaresima R, Grilli A, Tettamanti L, Vinci R, Sammartino G, Tetè S and Gherlone E: A comparison of bovine bone and hydroxyapatite scaffolds during initial bone regeneration: An in vitro evaluation. Implant Dent. 22:613–622. 2013.PubMed/NCBI View Article : Google Scholar

27 

Seibel MJ: Bone metabolism. In: Encyclopedic Reference of MolecularPharmacology. Springer, Berlin Heidelberg, Berlin, Heidelberg, pp190-197, 2004.

28 

Jolly JJ, Chin KY, Farhana MFN, Alias E, Chua KH, Hasan WNW and Ima-Nirwana S: Optimization of the Static Human Osteoblast/Osteoclast Co-culture System. Iran J Med Sci. 43:208–213. 2018.PubMed/NCBI

29 

Sulaiman SB, Keong TK, Cheng CH, Saim AB and Idrus RB: Tricalcium phosphate/hydroxyapatite (TCP-HA) bone scaffold as potential candidate for the formation of tissue engineered bone. Indian J Med Res. 137:1093–1101. 2013.PubMed/NCBI

30 

Setzer B, Bächle M, Metzger MC and Kohal RJ: The gene-expression and phenotypic response of hFOB 1.19 osteoblasts to surface-modified titanium and zirconia. Biomaterials. 30:979–990. 2009.PubMed/NCBI View Article : Google Scholar

31 

Majeed SA, Mohamed N and Soelaiman I-N: Quantification of Bone Histomorphometric Parameters Using the Weibel Technique in Animals. Med Health. 11:278–288. 2016.

32 

Siddiqui JA and Partridge NC: Physiological bone remodeling: Systemic regulation and growth factor involvement. Physiology (Bethesda). 31:233–245. 2016.PubMed/NCBI View Article : Google Scholar

33 

Thomson RC, Yaszemski MJ, Powers JM and Mikos AG: Fabrication of biodegradable polymer scaffolds to engineer trabecular bone. J Biomater Sci Polym Ed. 7:23–38. 1995.PubMed/NCBI View Article : Google Scholar

34 

Salgado AJ, Coutinho OP and Reis RL: Bone tissue engineering: State of the art and future trends. Macromol Biosci. 4:743–765. 2004.PubMed/NCBI View Article : Google Scholar

35 

Rashmi RP, Pathak R, Amarpal Aithal HP, Kinjavdekar P, Pawde AM, Tiwari AK, Sangeetha P, Tamilmahan P and Manzoor AB: Evaluation of tissue-engineered bone constructs using rabbit fetal osteoblasts on acellular bovine cancellous bone matrix. Vet World. 10:163–169. 2017.PubMed/NCBI View Article : Google Scholar

36 

Li Z, Ramay HR, Hauch KD, Xiao D and Zhang M: Chitosan-alginate hybrid scaffolds for bone tissue engineering. Biomaterials. 26:3919–3928. 2005.PubMed/NCBI View Article : Google Scholar

37 

Sladkova M, Cheng J, Palmer M, Chen S, Lin C, Xia W, Yu YE, Zhou B, Engqvist H and de Peppo GM: Comparison of decellularized cow and human bone for engineering bone grafts with human induced pluripotent stem cells. Tissue Eng Part A. 25:288–301. 2019.PubMed/NCBI View Article : Google Scholar

38 

Huang R-L, Tremp M, Ho C-K, Sun Y, Liu K and Li Q: Prefabrication of a functional bone graft with a pedicled periosteal flap as an in vivo bioreactor. Sci Rep. 7(18038)2017.PubMed/NCBI View Article : Google Scholar

39 

Andric T, Taylor BL, Whittington AR and Freeman JW: Fabrication and Characterization of Three-Dimensional Electrospun Scaffolds for Bone Tissue Engineering. Regen Eng Transl Med. 1:32–41. 2015.

40 

Liu X, Smith LA, Hu J and Ma PX: Biomimetic nanofibrous gelatin/apatite composite scaffolds for bone tissue engineering. Biomaterials. 30:2252–2258. 2009.PubMed/NCBI View Article : Google Scholar

41 

Porter BD, Oldham JB, He SL, Zobitz ME, Payne RG, An KN, Currier BL, Mikos AG and Yaszemski MJ: Mechanical properties of a biodegradable bone regeneration scaffold. J Biomech Eng. 122:286–288. 2000.PubMed/NCBI View Article : Google Scholar

42 

Chu T-MG, Orton DG, Hollister SJ, Feinberg SE and Halloran JW: Mechanical and in vivo performance of hydroxyapatite implants with controlled architectures. Biomaterials. 23:1283–1293. 2002.PubMed/NCBI View Article : Google Scholar

43 

Hutmacher DW: Scaffolds in tissue engineering bone and cartilage. In: The Biomaterials. Silver Jubilee Compendium Elsevier, pp175-189, 2006.

44 

Ma PX: Scaffolds for tissue fabrication. Mater Today. 7:30–40. 2004.

45 

Gaspar DA, Gomide V and Monteiro FJ: The role of perfusion bioreactors in bone tissue engineering. Biomatter. 2:167–175. 2012.PubMed/NCBI View Article : Google Scholar

46 

Ima Soelaiman S, Chua KH, Alias E, Chin KY, Jolly JJ and Mohd Fozi NF: A Method Of Preparing A Cell-Scaffold Composite For In-Vitro Study Of Therapeutic Agents On Bone Disorders (Filing ID by Universiti Kebangsaan Malaysia: PI2018701031).

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Copy and paste a formatted citation
Spandidos Publications style
Jolly JJ, Mohd Fozi N, Chin K, Wong SK, Chua KH, Alias E, Adnan NS and Ima‑Nirwana S: Skeletal microenvironment system utilising bovine bone scaffold co‑cultured with human osteoblasts and osteoclast‑like cells. Exp Ther Med 22: 680, 2021.
APA
Jolly, J.J., Mohd Fozi, N., Chin, K., Wong, S.K., Chua, K.H., Alias, E. ... Ima‑Nirwana, S. (2021). Skeletal microenvironment system utilising bovine bone scaffold co‑cultured with human osteoblasts and osteoclast‑like cells. Experimental and Therapeutic Medicine, 22, 680. https://doi.org/10.3892/etm.2021.10112
MLA
Jolly, J. J., Mohd Fozi, N., Chin, K., Wong, S. K., Chua, K. H., Alias, E., Adnan, N. S., Ima‑Nirwana, S."Skeletal microenvironment system utilising bovine bone scaffold co‑cultured with human osteoblasts and osteoclast‑like cells". Experimental and Therapeutic Medicine 22.1 (2021): 680.
Chicago
Jolly, J. J., Mohd Fozi, N., Chin, K., Wong, S. K., Chua, K. H., Alias, E., Adnan, N. S., Ima‑Nirwana, S."Skeletal microenvironment system utilising bovine bone scaffold co‑cultured with human osteoblasts and osteoclast‑like cells". Experimental and Therapeutic Medicine 22, no. 1 (2021): 680. https://doi.org/10.3892/etm.2021.10112
Copy and paste a formatted citation
x
Spandidos Publications style
Jolly JJ, Mohd Fozi N, Chin K, Wong SK, Chua KH, Alias E, Adnan NS and Ima‑Nirwana S: Skeletal microenvironment system utilising bovine bone scaffold co‑cultured with human osteoblasts and osteoclast‑like cells. Exp Ther Med 22: 680, 2021.
APA
Jolly, J.J., Mohd Fozi, N., Chin, K., Wong, S.K., Chua, K.H., Alias, E. ... Ima‑Nirwana, S. (2021). Skeletal microenvironment system utilising bovine bone scaffold co‑cultured with human osteoblasts and osteoclast‑like cells. Experimental and Therapeutic Medicine, 22, 680. https://doi.org/10.3892/etm.2021.10112
MLA
Jolly, J. J., Mohd Fozi, N., Chin, K., Wong, S. K., Chua, K. H., Alias, E., Adnan, N. S., Ima‑Nirwana, S."Skeletal microenvironment system utilising bovine bone scaffold co‑cultured with human osteoblasts and osteoclast‑like cells". Experimental and Therapeutic Medicine 22.1 (2021): 680.
Chicago
Jolly, J. J., Mohd Fozi, N., Chin, K., Wong, S. K., Chua, K. H., Alias, E., Adnan, N. S., Ima‑Nirwana, S."Skeletal microenvironment system utilising bovine bone scaffold co‑cultured with human osteoblasts and osteoclast‑like cells". Experimental and Therapeutic Medicine 22, no. 1 (2021): 680. https://doi.org/10.3892/etm.2021.10112
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