LncRNA LINC01535 promotes colorectal cancer development and chemoresistance by sponging miR‑761

Zhao,Changjie; Jiang,Qi; Chen,Lin; Chen,Wei

doi:10.3892/etm.2021.10117

LncRNA LINC01535 promotes colorectal cancer development and chemoresistance by sponging miR‑761

Authors:
- Changjie Zhao
- Qi Jiang
- Lin Chen
- Wei Chen
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Affiliations: Endoscopy Center, Affiliated Dongtai Hospital of Nantong University, Dongtai, Jiangsu 224200, P.R. China, Department of Gastroenterology, Affiliated Dongtai Hospital of Nantong University, Dongtai, Jiangsu 224200, P.R. China
Published online on: April 26, 2021 https://doi.org/10.3892/etm.2021.10117
Article Number: 685
Copyright: © Zhao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Colorectal cancer (CRC) is one of the most common human cancer types and a leading cause of cancer‑related death. Accumulating evidence has confirmed that long non‑coding RNAs have crucial roles in CRC progression. In the present study, the biological roles of LINC01535 were investigated and the interaction between long intergenic non‑coding RNA (LINC)01535 and microRNA (miR)‑761 in CRC was explored. LINC01535 expression was observed to be upregulated in CRC tissues and cell lines. A functional study suggested that LINC01535 silencing inhibited CRC cell proliferation and invasion but enhanced cisplatin sensitivity of CRC cells, while co‑transfection with a miR‑761 inhibitor reversed these biological effects. A luciferase reporter assay demonstrated that LINC01535 regulated miR‑761 directly and RNA‑binding protein immunoprecipitation further confirmed that the suppression of LINC01535 by miR‑761 was via an RNA‑induced silencing complex. Finally, knockdown of LINC01535 inhibited the growth of CRC cells in vivo. Collectively, the results suggested that LINC01535 exerts oncogenic functions in CRC by sponging miR‑761. In conclusion, the present study indicated that LINC01535 promoted CRC progression through sponging miR‑761, and may serve as a potential diagnostic biomarker and therapeutic target for CRC.

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