Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Experimental and Therapeutic Medicine
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-0981 Online ISSN: 1792-1015
Journal Cover
July-2021 Volume 22 Issue 1

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
July-2021 Volume 22 Issue 1

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article Open Access

Acute glucose fluctuation promotes in vitro intestinal epithelial cell apoptosis and inflammation via the NOX4/ROS/JAK/STAT3 signaling pathway

  • Authors:
    • Bingyu Chen
    • Yuanyuan Jia
    • Dongxue Lu
    • Zhiguang Sun
  • View Affiliations / Copyright

    Affiliations: Department of Gastroenterology, Nanjing University of Chinese Medicine, Nanjing, Jiangsu 210000, P.R. China, Department of Medical Oncology, The Second People's Hospital of Huai'an, Huaian, Jiangsu 223001, P.R. China
    Copyright: © Chen et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 688
    |
    Published online on: April 28, 2021
       https://doi.org/10.3892/etm.2021.10120
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

High blood glucose commonly occurs in patients with diabetes mellitus, but little is known of its effects on intestinal epithelial cells, or its associated mechanisms of action therein. In the present study, intestinal epithelial cells were assigned to five groups: i) The normal glucose (NG) group, incubated in 5.0 mmol/l glucose; ii) the constant high glucose (CHG) group, treated with 25.0 mmol/l glucose; iii) the intermittent high glucose (IHG) group, treated with alternating doses of 5.0 and 25.0 mmol/l glucose every 8 h; iv) the mannose group, cultured in 25.0 mmol/l mannose (the osmotic control); and v) the IHG glucose + GKT137831 group, pretreated with 100 nmol/l NADPH oxidase 4 (NOX4) inhibitor, GKT137831, and then exposed to IHG. TNF‑α, IL‑1 and IL‑6 levels were quantified using ELISA kits. Intestinal epithelial cell apoptosis was assessed by flow cytometry and oxidative stress was evaluated by reactive oxygen species (ROS) and malondialdehyde (MDA) detection. The expression levels of proteins associated with apoptosis and involved in the signal transduction of Janus kinase (JAK)/STAT3 pathway were assessed using western blot analysis. The results indicated that NOX4 expression was significantly higher in the CHG group than in the NG group (P<0.01), but lower than in the IHG group (P<0.001). The IHG group exhibited apoptosis and oxidative stress accompanied by the most significant increase in MDA, ROS and inflammatory cytokine levels (P<0.001), which was followed by that of the CHG group. Additionally, the IHG group exhibited reduced Bcl‑2, as well as enhanced Bax and cleaved caspase‑3 levels compared with the CHG group (P<0.001). Furthermore, the level of phosphorylated (p‑)JAK/p‑STAT3 was increased to a greater extent in the IHG group than in the CHG group (P<0.001). In conclusion, the findings of the present study indicated that CHG may trigger intestinal epithelial cell apoptosis and inflammation through the NOX4/ROS/JAK/STAT3 pathway, which may be aggravated by acute glucose fluctuation.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

View References

1 

Wilson PWF, D'Agostino RB, Helen P, Lisa S and Meigs JB: Metabolic syndrome as a precursor of cardiovascular disease and type 2 diabetes mellitus. Circulation. 112:3066–3072. 2005.PubMed/NCBI View Article : Google Scholar

2 

Brownlee M: Biochemistry and molecular cell biology of diabetic complications. Nature. 414:813–820. 2001.PubMed/NCBI View Article : Google Scholar

3 

Papatheodorou K, Banach M, Bekiari E, Rizzo M and Edmonds M: Complications of diabetes 2017. J Diabetes Res. 2018(3086167)2018.PubMed/NCBI View Article : Google Scholar

4 

Snelson M, de Pasquale C, Ekinci EI and Coughlan MT: Gut microbiome, prebiotics, intestinal permeability and diabetes complications. Best Pract Res Clin Endocrinol Metab, 2021 (Ahead of print).

5 

Talley NJ, Young L, Bytzer P, Hammer J, Leemon M, Jones M and Horowitz M: Impact of chronic gastrointestinal symptoms in diabetes mellitus on health-related quality of life. Am J Gastroenterol. 96:71–76. 2001.PubMed/NCBI View Article : Google Scholar

6 

Mooradian AD, Morley JE, Levine AS, Prigge WF and Gebhard RL: Abnormal intestinal permeability to sugars in diabetes mellitus. Diabetologia. 29:221–224. 1986.PubMed/NCBI View Article : Google Scholar

7 

Meddings JB, Jarand J, Urbanski SJ, Hardin J and Gall DG: Increased gastrointestinal permeability is an early lesion in the spontaneously diabetic BB rat. Am J Physiol. 276:951–957. 1999.PubMed/NCBI View Article : Google Scholar

8 

Neu J, Reverte CM, Mackey AD, Liboni K, Tuhacek-Tenace LM, Hatch M, Li N, Caicedo RA, Schatz DA and Atkinson M: Changes in intestinal morphology and permeability in the biobreeding rat before the onset of type 1 diabetes. J Pediatr Gastroenterol Nutr. 40:589–595. 2005.PubMed/NCBI View Article : Google Scholar

9 

Chocarro-Calvo A, García-Martínez JM, Ardila-González S, De la Vieja A and García-Jiménez C: Glucose-induced β-catenin acetylation enhances Wnt signaling in cancer. Mol Cell. 49:474–486. 2013.PubMed/NCBI View Article : Google Scholar

10 

Baynes JW and Thorpe SR: Role of oxidative stress in diabetic complications: A new perspective on an old paradigm. Diabetes. 43:1–9. 1999.PubMed/NCBI View Article : Google Scholar

11 

Babior BM: NADPH oxidase. Curr Opin Immunol. 16:42–47. 2004.PubMed/NCBI View Article : Google Scholar

12 

Ago T, Kuroda J, Kamouchi M, Sadoshima J and Kitazono T: Pathophysiological roles of NADPH oxidase/nox family proteins in the vascular system. Review and perspective. Circ J. 75:1791–1800. 2011.PubMed/NCBI View Article : Google Scholar

13 

Asaba K, Tojo A, Onozato ML, Goto A, Quinn MT, Fujita T and Wilcox CS: Effects of NADPH oxidase inhibitor in diabetic nephropathy. Kidney Int. 67:1890–1898. 2005.PubMed/NCBI View Article : Google Scholar

14 

Roe ND, Thomas DP and Ren J: Inhibition of NADPH oxidase alleviates experimental diabetes-induced myocardial contractile dysfunction. Diabetes Obes Metab. 13:465–473. 2011.PubMed/NCBI View Article : Google Scholar

15 

Teixeira G, Szyndralewiez C, Molango S, Carnesecchi S, Heitz F, Wiesel P and Wood JM: Therapeutic potential of NADPH oxidase 1/4 inhibitors. Br J Pharmacol. 174:1667–1669. 2017.PubMed/NCBI View Article : Google Scholar

16 

Wang JW, Pan YB, Cao YQ, Wang C, Jiang WD, Zhai WF and Lu JG: Loganin alleviates LPS-activated intestinal epithelial inflammation by regulating TLR4/NF-κB and JAK/STAT3 signaling pathways. Kaohsiung J Med Sci. 36:257–264. 2020.PubMed/NCBI View Article : Google Scholar

17 

Hirano T, Ishihara KM and Hibi M: Roles of STAT3 in mediating the cell growth, differentiation and survival signals relayed through the IL-6 family of cytokine receptors. Oncogene. 19:2548–2556. 2000.PubMed/NCBI View Article : Google Scholar

18 

Monnier L, Mas E, Ginet C, Michel F, Villon L, Cristol JP and Colette C: Activation of oxidative stress by acute glucose fluctuations compared with sustained chronic hyperglycemia in patients with type 2 diabetes. JAMA. 295:1681–1687. 2006.PubMed/NCBI View Article : Google Scholar

19 

Ceriello A, Esposit K, Piconi L, Ihnat MA, Thorpe JE, Testa R, Boemi M and Giugliano D: Oscillating glucose is more deleterious to endothelial function and oxidative stress than mean glucose in normal and type 2 diabetic patients. Diabetes. 57:1349–1354. 2008.PubMed/NCBI View Article : Google Scholar

20 

Lee MK, Kim IH, Choi YH and Nam TJ: A peptide from Porphyra yezoensisstimulates the proliferation of IEC-6 cells by activating the insulin-like growth factor I receptor signaling pathway. Int J Mol Med. 35:533–538. 2015.PubMed/NCBI View Article : Google Scholar

21 

Shen JT, Li YS, Xia ZQ, Wen SH, Yao X, Yang WJ, Li C and Liu KX: Remifentanil preconditioning protects the small intestine against ischemia/reperfusion injury via intestinal δ- and µ-opioid receptors. Surgery. 159:548–559. 2016.PubMed/NCBI View Article : Google Scholar

22 

Ying C, Wang S, Lu Y, Chen L, Mao Y, Ling H, Cheng X and Zhou X: Glucose fluctuation increased mesangial cell apoptosis related to AKT signal pathway. Arch Med Sci. 15:730–737. 2019.PubMed/NCBI View Article : Google Scholar

23 

Hsieh CF, Liu CK, Lee CT, Yu LE and Wang JY: Acute glucose fluctuation impacts microglial activity, leading to inflammatory activation or self-degradation. Sci Rep. 9(840)2019.PubMed/NCBI View Article : Google Scholar

24 

Quagliaro L, Piconi L, Assaloni R, Martinelli L, Motz E and Ceriello A: Intermittent high glucose enhances apoptosis related to oxidative stress in human umbilical vein endothelial cells: The Role of Protein Kinase C and NAD(P)H-Oxidase Activation. Diabetes. 52:2795–2804. 2003.PubMed/NCBI View Article : Google Scholar

25 

Cho JH, Chang SA, Kwon HS, Choi YH, Ko SH, Moon SD, Yoo SJ, Song KH, Son HS, Kim HS, et al: Long-term effect of the Internet-based glucose monitoring system on HbA1c reduction and glucose stability: A 30-month follow-up study for diabetes management with a ubiquitous medical care system. Diabetes Care. 29:2625–2631. 2006.PubMed/NCBI View Article : Google Scholar

26 

Liu Q, Huang QX, Lou FC, Zhang L and Hou WK, Yu S, Xu H, Wang Q, Zhang Y and Hou WK: Effects of glucose and insulin on the H9c2 (2-1) cell proliferation may be mediated through regulating glucose transporter 4 expression. Chin Med J (Engl). 126:4037–4042. 2013.PubMed/NCBI

27 

Lopez R, Arumugam A, Joseph R, Monga K, Boopalan T, Agullo P, Gutierrez C, Nandy S, Subramani R, de la Rosa JM and Lakshmanaswamy R: Hyperglycemia enhances the proliferation of non-tumorigenic and malignant Mammary epithelial cells through increased leptin/IGF1R signaling and activation of AKT/mTOR. PLoS One. 8(e79708)2013.PubMed/NCBI View Article : Google Scholar

28 

Ozmen A, Unek G, Kipmen-Korgun D and Korgun ET: The PI3K/Akt and MAPK-ERK1/2 pathways are altered in STZ induced diabetic rat placentas. Histol Histopathol. 29:743–756. 2013.PubMed/NCBI View Article : Google Scholar

29 

Adachi T, Mori C, Sakurai K, Shihara N, Tsuda K and Yasuda K: Morphological changes and increased sucrase and isomaltase activity in small intestines of insulin-deficient and type 2 diabetic rats. Endocr J. 50:271–279. 2003.PubMed/NCBI View Article : Google Scholar

30 

Rasool S, Kadla SA, Rasool V and Ganai BA: A comparative overview of general risk factors associated with the incidence of colorectal cancer. Tumor Biology. 34:2469–2476. 2013.PubMed/NCBI View Article : Google Scholar

31 

Hata K, Kubota M, Shimizu M, Moriwaki H, Kuno T, Tanaka T, Hara A and Hirose Y: Monosodium glutamate-induced diabetic mice are susceptible to azoxymethane-induced colon tumorigenesis. Carcinogenesis. 33:702–707. 2012.PubMed/NCBI View Article : Google Scholar

32 

King GL and Loeken MR: Hyperglycemia-induced oxidative stress in diabetic complications. Histochem Cell Biol. 122:333–338. 2004.PubMed/NCBI View Article : Google Scholar

33 

Wu N, Shen H, Liu H, Wang Y, Bai Y and Han P: Acute blood glucose fluctuation enhances rat aorta endothelial cell apoptosis, oxidative stress and pro-inflammatory cytokine expression in vivo. Cardiovasc Diabetol. 15(109)2016.PubMed/NCBI View Article : Google Scholar

34 

Sharma A, Tate M, Mathew G, Vince JE, Ritchie RH and Haan JB: Oxidative stress and NLRP3-inflammasome activity as significant drivers of diabetic cardiovascular complications: Therapeutic implications. Front Physiol. 9(114)2018.PubMed/NCBI View Article : Google Scholar

35 

Lal MA, Brismar H, Eklöf AC and Aperia A: Role of oxidative stress in advanced glycation end product-induced mesangial cell activation. Kidney Int. 61:2006–2014. 2002.PubMed/NCBI View Article : Google Scholar

36 

Zhang W, Zhao S, Li Y, Peng G and Han P: Acute blood glucose fluctuation induces myocardial apoptosis through oxidative stress and nuclear factor-ĸB activation. Cardiology. 124:11–17. 2013.PubMed/NCBI View Article : Google Scholar

37 

Xue B, Wang L, Zhang Z, Wang R, Xia XX, Han PP, Cao LJ, Liu YH and Sun LQ: Puerarin may protect against Schwann cell damage induced by glucose fluctuation. J Nat Med. 71:472–481. 2017.PubMed/NCBI View Article : Google Scholar

38 

Xia J, Hu S, Xu J, Hao H, Yin C and Xu D: The correlation between glucose fluctuation from self-monitored blood glucose and the major adverse cardiac events in diabetic patients with acute coronary syndrome during a 6-month follow-up by WeChat application. Clin Chem Lab Med. 56:2119–2124. 2018.PubMed/NCBI View Article : Google Scholar

39 

Glucose tolerance and mortality. Comparison of WHO and American Diabetes Association diagnostic criteria. The DECODE study group. European Diabetes Epidemiology Group. Diabetes Epidemiology: Collaborative analysis Of Diagnostic criteria in Europe. Lancet. 354:617–621. 1999.PubMed/NCBI

40 

Curtin JF, Donovan M and Cotter TG: Regulation and measurement of oxidative stress in apoptosis. J Immunol Methods. 265:49–72. 2002.PubMed/NCBI View Article : Google Scholar

41 

Nikoletopoulou V, Markaki M, Palikaras K and Tavernarakis N: Crosstalk between apoptosis, necrosis and autophagy. Biochim Biophys Acta. 1833:3448–3459. 2013.PubMed/NCBI View Article : Google Scholar

42 

Hussain T, Tan B, Yin Y, Blachier F, Tossou MC and Rahu N: Oxidative stress and inflammation: What Polyphenols Can Do for Us? Oxid Med Cell Longev. 2016(7432797)2016.PubMed/NCBI View Article : Google Scholar

43 

Shen Y, Zhang Q, Huang Z, Zhu J, Qiu J, Ma W, Yang X, Ding F and Sun H: Isoquercitrin delays denervated soleus muscle atrophy by inhibiting oxidative stress and inflammation. Front Physiol. 11(988)2020.PubMed/NCBI View Article : Google Scholar

44 

Meng XM, Ren GL, Gao L, Yang Q, Li HD, Wu WF, Huang C, Zhang L, Lv XW and Li J: NADPH oxidase 4 promotes cisplatin-induced acute kidney injury via ROS-mediated programmed cell death and inflammation. Lab Invest. 98:63–78. 2018.PubMed/NCBI View Article : Google Scholar

45 

Sedeek M, Montezano AC, Hebert RL, Gray SP, Di Marco E, Jha JC, Cooper ME, Jandeleit-Dahm K, Schiffrin EL, Wilkinson-Berka JL and Touyz RM: Oxidative stress, Nox isoforms and complications of diabetes-potential targets for novel therapies. J Cardiovasc Transl Res. 5:509–518. 2012.PubMed/NCBI View Article : Google Scholar

46 

Sedeek M, Nasrallah R, Touyz RM and Hebert RL: NADPH oxidases, reactive oxygen species, and the kidney: Friend and foe. J Am Soc Nephrol. 24:1512–1518. 2013.PubMed/NCBI View Article : Google Scholar

47 

Chu FF, Esworthy RS, Shen B, Gao Q and Doroshow JH: Dexamethasone and Tofacitinib suppress NADPH oxidase expression and alleviate very-early-onset ileocolitis in mice deficient in GSH peroxidase 1 and 2. Life Sci. 239(116884)2019.PubMed/NCBI View Article : Google Scholar

48 

Lindquist RL, Bayat-Sarmadi J, Leben R, Niesner R and Hauser AE: NAD(P)H oxidase activity in the small intestine is predominantly found in enterocytes, not professional phagocytes. Int J Mol Sci. 19(1365)2018.PubMed/NCBI View Article : Google Scholar

49 

Yan J, Wang C, Jin Y, Meng Q, Liu Q, Liu Z, Liu K and Sun H: Catalpol ameliorates hepatic insulin resistance in type 2 diabetes through acting on AMPK/NOX4/PI3K/AKT pathway. Pharmacol Res. 130:466–480. 2018.PubMed/NCBI View Article : Google Scholar

50 

Sun L, Li W, Li W, Xiong L, Li G and Ma R: Astragaloside IV prevents damage to human mesangial cells through the inhibition of the NADPH oxidase/ROS/Akt/NF-κB pathway under high glucose conditions. Int J Mol Med. 34:167–176. 2014.PubMed/NCBI View Article : Google Scholar

51 

Chen F, Qian LH, Deng B, Liu ZM, Zhao Y and Le YY: Resveratrol protects vascular endothelial cells from high glucose-induced apoptosis through inhibition of NADPH oxidase activation-driven oxidative stress. CNS Neurosci Ther. 19:675–681. 2013.PubMed/NCBI View Article : Google Scholar

52 

Chowdhury SR, Saleh A, Akude E, Smith DR, Morrow D, Tessler L, Calcutt NA and Fernyhough P: Ciliary neurotrophic factor reverses aberrant mitochondrial bioenergetics through the JAK/STAT pathway in cultured sensory neurons derived from streptozotocin-induced diabetic rodents. Cell Mol Neurobiol. 34:643–649. 2014.PubMed/NCBI View Article : Google Scholar

53 

Li Q, Lin Y, Wang S, Zhang L and Guo L: GLP-1 inhibits high-glucose-induced oxidative injury of vascular endothelial cells. Sci Rep. 7(8008)2017.PubMed/NCBI View Article : Google Scholar

54 

Liu M, Yan L, Liang B, Li Z, Jiang Z, Chu C and Yang J: Hydrogen sulfide attenuates myocardial fibrosis in diabetic rats through the JAK/STAT signaling pathway. Int J Mol Med. 41:1867–1876. 2018.PubMed/NCBI View Article : Google Scholar

55 

Marrero MB, Banes-Berceli AK, Stern DM and Eaton DC: Role of the JAK/STAT signaling pathway in diabetic nephropathy. Am J Physiol Renal Physiol. 290:F762–F768. 2006.PubMed/NCBI View Article : Google Scholar

56 

Wang X, Shaw S, Amiri F, Eaton DC and Marrero MB: Inhibition of the Jak/STAT signaling pathway prevents the high glucose-induced increase in tgf-beta and fibronectin synthesis in mesangial cells. Diabetes. 51:3505–3509. 2002.PubMed/NCBI View Article : Google Scholar

57 

Yoshikawa H, Matsubara K, Qian GS, Jackson P, Groopman JD, Manning JE, Harris CC and Herman JG: SOCS-1, a negative regulator of the JAK/STAT pathway, is silenced by methylation in human hepatocellular carcinoma and shows growth-suppression activity. Nat Genet. 28:29–35. 2001.PubMed/NCBI View Article : Google Scholar

58 

Das A, Salloum FN, Durrant D, Ockaili R and Kukreja RC: Rapamycin protects against myocardial ischemia-reperfusion injury through JAK2-STAT3 signaling pathway. J Mol Cell Cardiol. 53:858–869. 2012.PubMed/NCBI View Article : Google Scholar

59 

Sun X, Chen RC, Yang ZH, Sun GB, Wang M, Ma XJ, Yang LJ and Sun XB: Taxifolin prevents diabetic cardiomyopathy in vivo and in vitro by inhibition of oxidative stress and cell apoptosis. Food Chem Toxicol. 63:221–232. 2014.PubMed/NCBI View Article : Google Scholar

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Chen B, Jia Y, Lu D and Sun Z: Acute glucose fluctuation promotes <em>in vitro</em> intestinal epithelial cell apoptosis and inflammation via the NOX4/ROS/JAK/STAT3 signaling pathway. Exp Ther Med 22: 688, 2021.
APA
Chen, B., Jia, Y., Lu, D., & Sun, Z. (2021). Acute glucose fluctuation promotes <em>in vitro</em> intestinal epithelial cell apoptosis and inflammation via the NOX4/ROS/JAK/STAT3 signaling pathway. Experimental and Therapeutic Medicine, 22, 688. https://doi.org/10.3892/etm.2021.10120
MLA
Chen, B., Jia, Y., Lu, D., Sun, Z."Acute glucose fluctuation promotes <em>in vitro</em> intestinal epithelial cell apoptosis and inflammation via the NOX4/ROS/JAK/STAT3 signaling pathway". Experimental and Therapeutic Medicine 22.1 (2021): 688.
Chicago
Chen, B., Jia, Y., Lu, D., Sun, Z."Acute glucose fluctuation promotes <em>in vitro</em> intestinal epithelial cell apoptosis and inflammation via the NOX4/ROS/JAK/STAT3 signaling pathway". Experimental and Therapeutic Medicine 22, no. 1 (2021): 688. https://doi.org/10.3892/etm.2021.10120
Copy and paste a formatted citation
x
Spandidos Publications style
Chen B, Jia Y, Lu D and Sun Z: Acute glucose fluctuation promotes <em>in vitro</em> intestinal epithelial cell apoptosis and inflammation via the NOX4/ROS/JAK/STAT3 signaling pathway. Exp Ther Med 22: 688, 2021.
APA
Chen, B., Jia, Y., Lu, D., & Sun, Z. (2021). Acute glucose fluctuation promotes <em>in vitro</em> intestinal epithelial cell apoptosis and inflammation via the NOX4/ROS/JAK/STAT3 signaling pathway. Experimental and Therapeutic Medicine, 22, 688. https://doi.org/10.3892/etm.2021.10120
MLA
Chen, B., Jia, Y., Lu, D., Sun, Z."Acute glucose fluctuation promotes <em>in vitro</em> intestinal epithelial cell apoptosis and inflammation via the NOX4/ROS/JAK/STAT3 signaling pathway". Experimental and Therapeutic Medicine 22.1 (2021): 688.
Chicago
Chen, B., Jia, Y., Lu, D., Sun, Z."Acute glucose fluctuation promotes <em>in vitro</em> intestinal epithelial cell apoptosis and inflammation via the NOX4/ROS/JAK/STAT3 signaling pathway". Experimental and Therapeutic Medicine 22, no. 1 (2021): 688. https://doi.org/10.3892/etm.2021.10120
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team