Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Experimental and Therapeutic Medicine
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-0981 Online ISSN: 1792-1015
Journal Cover
July-2021 Volume 22 Issue 1

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
July-2021 Volume 22 Issue 1

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article Open Access

Features of a simvastatin‑loaded multi‑layered co‑electrospun barrier membrane for guided bone regeneration

  • Authors:
    • Dan Yu
    • Chongshang Huang
    • Chu Jiang
    • Huiyong Zhu
  • View Affiliations / Copyright

    Affiliations: Department of Oral and Maxillofacial Surgery, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang 310003, P.R. China, Department of Stomatology, Jiangshan People's Hospital, Jiangshan, Zhejiang 324100, P.R. China
    Copyright: © Yu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 713
    |
    Published online on: May 3, 2021
       https://doi.org/10.3892/etm.2021.10145
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

A novel tri‑layer membrane consisting of polycaprolactone (PCL) fibrous sheets and structured nanofibers with a gelatin (Gt) shell and a simvastatin‑containing PCL core (PCL‑Gt/PCL‑simvastatin membrane) was prepared. The soft external layer comprised of Gt/PCL‑simvastatin, the external layer of PCL and the middle layer of both microfilaments, interwoven together. The membrane was designed to promote osteoinduction and act as a barrier against cells but not against water and molecules in order to promote guided bone regeneration. The structure of the membrane was characterized by scanning electronic microscopy. The in vitro release rates of simvastatin over 32 days were determined by high‑performance liquid chromatography. For in vitro biological assays, bone marrow mesenchymal stem cells and human fibroblasts were cultured on the different surfaces of the membrane. Cell adhesion, proliferation, distribution, and differentiation were examined. For in vivo testing, cranial defects were created in rabbits to assess the amount of new bone formed for each membrane. The results revealed that membranes with multi‑layered structures showed good cell viability and effective osteoinductive and barrier properties. These results suggest that the novel multi‑layered PCL‑Gt/PCL‑simvastatin membranes have great potential for bone tissue engineering.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Figure 6

Figure 7

Figure 8

Figure 9

Figure 10

Figure 11

Figure 12

View References

1 

Rezvani Z, Venugopal JR, Urbanska AM, Mills DK, Ramakrishna S and Mozafari M: A bird's eye view on the use of electrospun nanofibrous scaffolds for bone tissue engineering: Current state-of-the-art, emerging directions and future trends. Nanomedicine. 12:2181–2200. 2016.PubMed/NCBI View Article : Google Scholar

2 

Yang G, Li X, He Y, Ma J, Ni G and Zhou S: From nano to micro to macro: Electrospun hierarchically structured polymeric fibers for biomedical applications. Prog Polymer Sci. 81:80–113. 2018.

3 

Jang JH, Castano O and Kim HW: Electrospun materials as potential platforms for bone tissue engineering. Adv Drug Deliv Rev. 61:1065–1083. 2009.PubMed/NCBI View Article : Google Scholar

4 

Holzwarth JM and Ma PX: Biomimetic nanofibrous scaffolds for bone tissue engineering. Biomaterials. 32:9622–9629. 2011.PubMed/NCBI View Article : Google Scholar

5 

Mata A, Geng Y, Henrikson KJ, Aparicio C, Stock SR, Satcher RL and Stupp SI: Bone regeneration mediated by biomimetic mineralization of a nanofiber matrix. Biomaterials. 31:6004–6012. 2010.PubMed/NCBI View Article : Google Scholar

6 

Li C, Vepari C, Jin HJ, Kim HJ and Kaplan DL: Electrospun silk-BMP-2 scaffolds for bone tissue engineering. Biomaterials. 27:3115–3124. 2006.PubMed/NCBI View Article : Google Scholar

7 

Sharifi F, Atyabi SM, Irani S and Bakhshi H: Bone morphogenic protein-2 immobilization by cold atmospheric plasma to enhance the osteoinductivity of carboxymethyl chitosan-based nanofibers. Carbohydr Polym. 231(115681)2020.PubMed/NCBI View Article : Google Scholar

8 

Kostopoulos L and Karring T: Augmentation of the rat mandible using guided tissue regeneration. Clin Oral Implants Res. 5:75–82. 1994.PubMed/NCBI View Article : Google Scholar

9 

Sedghi R, Shaabani A and Sayyari N: Electrospun triazole-based chitosan nanofibers as a novel scaffolds for bone tissue repair and regeneration. Carbohydr Polym. 230(115707)2020.PubMed/NCBI View Article : Google Scholar

10 

Elgali I, Turri A, Xia W, Norlindh B, Johansson A, Dahlin C, Thomsen P and Omar O: Guided bone regeneration using resorbable membrane and different bone substitutes: Early histological and molecular events. Acta Biomater. 29:409–423. 2016.PubMed/NCBI View Article : Google Scholar

11 

Samavedi S, Olsen Horton C, Guelcher SA, Goldstein AS and Whittington AR: Fabrication of a model continuously graded co-electrospun mesh for regeneration of the ligament-bone interface. Acta Biomater. 7:4131–4138. 2011.PubMed/NCBI View Article : Google Scholar

12 

Dalgic AD, Atila D, Karatas A, Tezcaner A and Keskin D: Diatom shell incorporated PHBV/PCL-pullulan co-electrospun scaffold for bone tissue engineering. Mater Sci Eng C Mater Biol Appl. 100:735–746. 2019.PubMed/NCBI View Article : Google Scholar

13 

Wang Y, Cui W, Zhao X, Wen S, Sun Y, Han J and Zhang H: Bone remodeling-inspired dual delivery electrospun nanofibers for promoting bone regeneration. Nanoscale. 11:60–71. 2018.PubMed/NCBI View Article : Google Scholar

14 

Liu S, Dong C, Lu G, Lu Q, Li Z, Kaplan DL and Zhu H: Bilayered vascular grafts based on silk proteins. Acta Biomater. 9:8991–9003. 2013.PubMed/NCBI View Article : Google Scholar

15 

Wu T, Zhang J, Wang Y, Li D, Sun B, El-Hamshary H, Yin M and Mo X: Fabrication and preliminary study of a biomimetic tri-layer tubular graft based on fibers and fiber yarns for vascular tissue engineering. Mater Sci Eng C Mater Biol Appl. 82:121–129. 2018.PubMed/NCBI View Article : Google Scholar

16 

Han F, Jia X, Dai D, Yang X, Zhao J, Zhao Y, Fan Y and Yuan X: Performance of a multilayered small-diameter vascular scaffold dual-loaded with VEGF and PDGF. Biomaterials. 34:7302–7313. 2013.PubMed/NCBI View Article : Google Scholar

17 

de Valence S, Tille JC, Giliberto JP, Mrowczynski W, Gurny R, Walpoth BH and Möller M: Advantages of bilayered vascular grafts for surgical applicability and tissue regeneration. Acta Biomater. 8:3914–3920. 2012.PubMed/NCBI View Article : Google Scholar

18 

Attalla R, Puersten E, Jain N and Selvaganapathy PR: 3D bioprinting of heterogeneous bi- and tri-layered hollow channels within gel scaffolds using scalable multi-axial microfluidic extrusion nozzle. Biofabrication. 11(015012)2018.PubMed/NCBI View Article : Google Scholar

19 

Blakeney BA, Tambralli A, Anderson JM, Andukuri A, Lim DJ, Dean DR and Jun HW: Cell infiltration and growth in a low density, uncompressed three-dimensional electrospun nanofibrous scaffold. Biomaterials. 32:1583–1590. 2011.PubMed/NCBI View Article : Google Scholar

20 

Wu T, Huang C, Li D, Yin A, Liu W, Wang J, Chen J, Ei-Hamshary H, Al-Deyab SS and Mo X: A multi-layered vascular scaffold with symmetrical structure by bi-directional gradient electrospinning. Colloids Surf B Biointerfaces. 133:179–188. 2015.PubMed/NCBI View Article : Google Scholar

21 

Garrett IR, Gutierrez G and Mundy GR: Statins and bone formation. Curr Pharm Des. 7:715–736. 2001.PubMed/NCBI View Article : Google Scholar

22 

Mundy G, Garrett R, Harris S, Chan J, Chen D, Rossini G, Boyce B, Zhao M and Gutierrez G: Stimulation of bone formation in vitro and in rodents by statins. Science. 286:1946–1949. 1999.PubMed/NCBI View Article : Google Scholar

23 

Thylin MR, McConnell JC, Schmid MJ, Reckling RR, Ojha J, Bhattacharyya I, Marx DB and Reinhardt RA: Effects of simvastatin gels on murine calvarial bone. J Periodontol. 73:1141–1148. 2002.PubMed/NCBI View Article : Google Scholar

24 

Jiang H, Hu Y, Li Y, Zhao P, Zhu K and Chen W: A facile technique to prepare biodegradable coaxial electrospun nanofibers for controlled release of bioactive agents. J Control Release. 108:237–243. 2005.PubMed/NCBI View Article : Google Scholar

25 

Zhu H, Yu D, Zhou Y, Wang C, Gao M, Jiang H and Wang H: Biological activity of a nanofibrous barrier membrane containing bone morphogenetic protein formed by core-shell electrospinning as a sustained delivery vehicle. J Biomed Mater Res B Appl Biomater. 101:541–552. 2013.PubMed/NCBI View Article : Google Scholar

26 

Chen H, Malheiro A, van Blitterswijk C, Mota C, Wieringa PA and Moroni L: Direct writing electrospinning of scaffolds with multidimensional fiber architecture for hierarchical tissue engineering. ACS Appl Mater Interfaces. 9:38187–38200. 2017.PubMed/NCBI View Article : Google Scholar

27 

Zhang X, Aubin JE and Inman RD: Molecular and cellular biology of new bone formation: Insights into the ankylosis of ankylosing spondylitis. Curr Opin Rheumatol. 15:387–393. 2003.PubMed/NCBI View Article : Google Scholar

28 

Lee JS, Lee JM and Im GI: Electroporation-mediated transfer of Runx2 and Osterix genes to enhance osteogenesis of adipose stem cells. Biomaterials. 32:760–768. 2011.PubMed/NCBI View Article : Google Scholar

29 

Wrobel E, Leszczynska J and Brzoska E: The characteristics of human bone-derived cells (HBDCS) during osteogenesis in vitro. Cell Mol Biol Lett. 21(26)2016.PubMed/NCBI View Article : Google Scholar

30 

Termine JD, Kleinman HK, Whitson SW, Conn KM, McGarvey ML and Martin GR: Osteonectin, a bone-specific protein linking mineral to collagen. Cell. 26:99–105. 1981.PubMed/NCBI View Article : Google Scholar

31 

Graneli C, Thorfve A, Ruetschi U, Brisby H, Thomsen P, Lindahl A and Karlsson C: Novel markers of osteogenic and adipogenic differentiation of human bone marrow stromal cells identified using a quantitative proteomics approach. Stem Cell Res. 12:153–165. 2014.PubMed/NCBI View Article : Google Scholar

32 

Pfaffl MW: A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Res. 29(e45)2001.PubMed/NCBI View Article : Google Scholar

33 

Bacevic M, Brkovic B, Lambert F, Djukic L, Petrovic N and Roganovic J: Leukocyte- and platelet-rich fibrin as graft material improves microRNA-21 expression and decreases oxidative stress in the calvarial defects of diabetic rabbits. Arch Oral Biol. 102:231–237. 2019.PubMed/NCBI View Article : Google Scholar

34 

Chen P and Liu B: Study on repair of critical calvarial defects with nano-hydroxyapatite/collagen/polylactic acid material compounded recombinant human bone morphogenetic protein 2 in rabbits. Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 21:1191–1195. 2007.PubMed/NCBI(In Chinese).

35 

Durmus E, Celik I, Aydin MF, Yildirim G and Sur E: Evaluation of the biocompatibility and osteoproductive activity of ostrich eggshell powder in experimentally induced calvarial defects in rabbits. J Biomed Mater Res B Appl Biomater. 86:82–89. 2008.PubMed/NCBI View Article : Google Scholar

36 

Li G, Wang X, Cao J, Ju Z, Ma D, Liu Y and Zhang J: Coculture of peripheral blood CD34+ cell and mesenchymal stem cell sheets increase the formation of bone in calvarial critical-size defects in rabbits. Br J Oral Maxillofac Surg. 52:134–139. 2014.PubMed/NCBI View Article : Google Scholar

37 

Swain LD, Cornet DA, Manwaring ME, Collins B, Singh VK, Beniker D and Carnes DL: Negative pressure therapy stimulates healing of critical-size calvarial defects in rabbits. Bonekey Rep. 2(299)2013.PubMed/NCBI View Article : Google Scholar

38 

Tuusa SM, Peltola MJ, Tirri T, Puska MA, Roytta M, Aho H, Sandholm J, Lassila LVJ and Vallittu PK: Reconstruction of critical size calvarial bone defects in rabbits with glass-fiber-reinforced composite with bioactive glass granule coating. J Biomed Mater Res B Appl Biomater. 84:510–519. 2008.PubMed/NCBI View Article : Google Scholar

39 

Wang Z, Han L, Sun T, Wang W, Li X and Wu B: Osteogenic and angiogenic lineage differentiated adipose-derived stem cells for bone regeneration of calvarial defects in rabbits. J Biomed Mater Res A. 109:538–550. 2021.PubMed/NCBI View Article : Google Scholar

40 

Wang Z, Hu H, Li Z, Weng Y, Dai T, Zong C, Liu Y and Liu B: Sheet of osteoblastic cells combined with platelet-rich fibrin improves the formation of bone in critical-size calvarial defects in rabbits. Br J Oral Maxillofac Surg. 54:316–321. 2016.PubMed/NCBI View Article : Google Scholar

41 

Kammerer PW, Lehnert M, Al-Nawas B, Kumar VV, Hagmann S, Alshihri A, Frerich B and Veith M: Osseoconductivity of a specific streptavidin-biotin-fibronectin surface coating of biotinylated titanium implants-a rabbit animal study. Clin Implant Dent Relat Res. 17 (Suppl 2):e601–e612. 2015.PubMed/NCBI View Article : Google Scholar

42 

Chen Z, Wang L and Jiang H: The effect of procyanidine crosslinking on the properties of the electrospun gelatin membranes. Biofabrication. 4(035007)2012.PubMed/NCBI View Article : Google Scholar

43 

Chen Z, Cao L, Wang L, Zhu H and Jiang H: Effect of fiber structure on the properties of the electrospun hybrid membranes composed of poly(ε-caprolactone) and gelatin. J Appl Polymer Sci. 127:4225–4232. 2013.

44 

McHugh J: Promising drug delivery system. Nat Rev Rheumatol. 15(64)2019.PubMed/NCBI View Article : Google Scholar

45 

Amschler K, Erpenbeck L, Kruss S and Schon MP: Nanoscale integrin ligand patterns determine melanoma cell behavior. ACS Nano. 8:9113–9125. 2014.PubMed/NCBI View Article : Google Scholar

46 

Qian C, Zhu C, Yu W, Jiang X and Zhang F: High-Fat diet/low-dose streptozotocin-induced type 2 diabetes in rats impacts osteogenesis and wnt signaling in bone marrow stromal cells. PLoS One. 10(e0136390)2015.PubMed/NCBI View Article : Google Scholar

47 

Seif S, Planz V and Windbergs M: Delivery of therapeutic proteins using electrospun fibers-recent developments and current challenges. Arch Pharm (Weinheim). 350(1700077)2017.PubMed/NCBI View Article : Google Scholar

48 

Yin L, Wang K, Lv X, Sun R, Yang S, Yang Y, Liu Y, Liu J, Zhou J and Yu Z: The fabrication of an ICA-SF/PLCL nanofibrous membrane by coaxial electrospinning and its effect on bone regeneration in vitro and in vivo. Sci Rep. 7(8616)2017.PubMed/NCBI View Article : Google Scholar

49 

Bigi A, Cojazzi G, Panzavolta S, Rubini K and Roveri N: Mechanical and thermal properties of gelatin films at different degrees of glutaraldehyde crosslinking. Biomaterials. 22:763–768. 2001.PubMed/NCBI View Article : Google Scholar

50 

Yi H, Ur Rehman F, Zhao C, Liu B and He N: Recent advances in nano scaffolds for bone repair. Bone Res. 4(16050)2016.PubMed/NCBI View Article : Google Scholar

51 

Gilbert TW, Stewart-Akers AM and Badylak SF: A quantitative method for evaluating the degradation of biologic scaffold materials. Biomaterials. 28:147–150. 2007.PubMed/NCBI View Article : Google Scholar

52 

Sung HJ, Meredith C, Johnson C and Galis ZS: The effect of scaffold degradation rate on three-dimensional cell growth and angiogenesis. Biomaterials. 25:5735–5742. 2004.PubMed/NCBI View Article : Google Scholar

53 

Alsberg E, Kong HJ, Hirano Y, Smith MK, Albeiruti A and Mooney DJ: Regulating bone formation via controlled scaffold degradation. J Dent Res. 82:903–908. 2003.PubMed/NCBI View Article : Google Scholar

54 

Zhang B, Zhang PB, Wang ZL, Lyu ZW and Wu H: Tissue-engineered composite scaffold of poly(lactide-co-glycolide) and hydroxyapatite nanoparticles seeded with autologous mesenchymal stem cells for bone regeneration. J Zhejiang Univ Sci B. 18:963–976. 2017.PubMed/NCBI View Article : Google Scholar

55 

Gong YY, Xue JX, Zhang WJ, Zhou GD, Liu W and Cao Y: A sandwich model for engineering cartilage with acellular cartilage sheets and chondrocytes. Biomaterials. 32:2265–2273. 2011.PubMed/NCBI View Article : Google Scholar

56 

Yin L, Yang S, He M, Chang Y, Wang K, Zhu Y, Liu Y, Chang Y and Yu Z: Physicochemical and biological characteristics of BMP-2/IGF-1-loaded three-dimensional coaxial electrospun fibrous membranes for bone defect repair. J Mater Sci Mater Med. 28(94)2017.PubMed/NCBI View Article : Google Scholar

57 

Mi R, Liu Y, Chen X and Shao Z: Structure and properties of various hybrids fabricated by silk nanofibrils and nanohydroxyapatite. Nanoscale. 8:20096–20102. 2016.PubMed/NCBI View Article : Google Scholar

58 

Teo BK, Wong ST, Lim CK, Kung TY, Yap CH, Ramagopal Y, Romer LH and Yim EKF: Nanotopography modulates mechanotransduction of stem cells and induces differentiation through focal adhesion kinase. ACS Nano. 7:4785–4798. 2013.PubMed/NCBI View Article : Google Scholar

59 

Stein D, Lee Y, Schmid MJ, Killpack B, Genrich MA, Narayana N, Marx DB, Cullen DM and Reinhardt RA: Local simvastatin effects on mandibular bone growth and inflammation. J Periodontol. 76:1861–1870. 2005.PubMed/NCBI View Article : Google Scholar

60 

Khan H, Mafi P, Mafi R and Khan W: The effects of ageing on differentiation and characterisation of human mesenchymal stem cells. Curr Stem Cell Res Ther. 13:378–383. 2018.PubMed/NCBI View Article : Google Scholar

61 

Prall WC, Saller MM, Scheumaier A, Tucholski T, Taha S, Bocker W and Polzer H: Proliferative and osteogenic differentiation capacity of mesenchymal stromal cells: Influence of harvesting site and donor age. Injury. 49:1504–1512. 2018.PubMed/NCBI View Article : Google Scholar

62 

Mendes SC, Tibbe JM, Veenhof M, Bakker K, Both S, Platenburg PP, Oner FC, de Bruijn JD and van Blitterswijk CA: Bone tissue-engineered implants using human bone marrow stromal cells: Effect of culture conditions and donor age. Tissue Eng. 8:911–920. 2002.PubMed/NCBI View Article : Google Scholar

63 

Hu ML: Osteogenic differentiation ability and related gene profiles of bone marrow mesenchymal stem cells derived from different ages (unpublished PhD thesis). Tianjin Medical University, 2019.

64 

Choudhery MS, Khan M, Mahmood R, Mehmood A, Khan SN and Riazuddin S: Bone marrow derived mesenchymal stem cells from aged mice have reduced wound healing, angiogenesis, proliferation and anti-apoptosis capabilities. Cell Biol Int. 36:747–753. 2012.PubMed/NCBI View Article : Google Scholar

65 

Fossett E, Khan WS, Pastides P and Adesida AB: The effects of ageing on proliferation potential, differentiation potential and cell surface characterisation of human mesenchymal stem cells. Curr Stem Cell Res Ther. 7:282–286. 2012.PubMed/NCBI View Article : Google Scholar

66 

Stolzing A, Jones E, McGonagle D and Scutt A: Age-related changes in human bone marrow-derived mesenchymal stem cells: Consequences for cell therapies. Mech Ageing Dev. 129:163–173. 2008.PubMed/NCBI View Article : Google Scholar

67 

Aksoy C, Kaya FA, Kuskonmaz BB, Uckan D and Severcan F: Structural investigation of donor age effect on human bone marrow mesenchymal stem cells: FTIR spectroscopy and imaging. Age (Dordr). 36(9691)2014.PubMed/NCBI View Article : Google Scholar

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Yu D, Huang C, Jiang C and Zhu H: Features of a simvastatin‑loaded multi‑layered co‑electrospun barrier membrane for guided bone regeneration. Exp Ther Med 22: 713, 2021.
APA
Yu, D., Huang, C., Jiang, C., & Zhu, H. (2021). Features of a simvastatin‑loaded multi‑layered co‑electrospun barrier membrane for guided bone regeneration. Experimental and Therapeutic Medicine, 22, 713. https://doi.org/10.3892/etm.2021.10145
MLA
Yu, D., Huang, C., Jiang, C., Zhu, H."Features of a simvastatin‑loaded multi‑layered co‑electrospun barrier membrane for guided bone regeneration". Experimental and Therapeutic Medicine 22.1 (2021): 713.
Chicago
Yu, D., Huang, C., Jiang, C., Zhu, H."Features of a simvastatin‑loaded multi‑layered co‑electrospun barrier membrane for guided bone regeneration". Experimental and Therapeutic Medicine 22, no. 1 (2021): 713. https://doi.org/10.3892/etm.2021.10145
Copy and paste a formatted citation
x
Spandidos Publications style
Yu D, Huang C, Jiang C and Zhu H: Features of a simvastatin‑loaded multi‑layered co‑electrospun barrier membrane for guided bone regeneration. Exp Ther Med 22: 713, 2021.
APA
Yu, D., Huang, C., Jiang, C., & Zhu, H. (2021). Features of a simvastatin‑loaded multi‑layered co‑electrospun barrier membrane for guided bone regeneration. Experimental and Therapeutic Medicine, 22, 713. https://doi.org/10.3892/etm.2021.10145
MLA
Yu, D., Huang, C., Jiang, C., Zhu, H."Features of a simvastatin‑loaded multi‑layered co‑electrospun barrier membrane for guided bone regeneration". Experimental and Therapeutic Medicine 22.1 (2021): 713.
Chicago
Yu, D., Huang, C., Jiang, C., Zhu, H."Features of a simvastatin‑loaded multi‑layered co‑electrospun barrier membrane for guided bone regeneration". Experimental and Therapeutic Medicine 22, no. 1 (2021): 713. https://doi.org/10.3892/etm.2021.10145
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team