Open Access

Features of a simvastatin‑loaded multi‑layered co‑electrospun barrier membrane for guided bone regeneration

  • Authors:
    • Dan Yu
    • Chongshang Huang
    • Chu Jiang
    • Huiyong Zhu
  • View Affiliations

  • Published online on: May 3, 2021     https://doi.org/10.3892/etm.2021.10145
  • Article Number: 713
  • Copyright: © Yu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

A novel tri‑layer membrane consisting of polycaprolactone (PCL) fibrous sheets and structured nanofibers with a gelatin (Gt) shell and a simvastatin‑containing PCL core (PCL‑Gt/PCL‑simvastatin membrane) was prepared. The soft external layer comprised of Gt/PCL‑simvastatin, the external layer of PCL and the middle layer of both microfilaments, interwoven together. The membrane was designed to promote osteoinduction and act as a barrier against cells but not against water and molecules in order to promote guided bone regeneration. The structure of the membrane was characterized by scanning electronic microscopy. The in vitro release rates of simvastatin over 32 days were determined by high‑performance liquid chromatography. For in vitro biological assays, bone marrow mesenchymal stem cells and human fibroblasts were cultured on the different surfaces of the membrane. Cell adhesion, proliferation, distribution, and differentiation were examined. For in vivo testing, cranial defects were created in rabbits to assess the amount of new bone formed for each membrane. The results revealed that membranes with multi‑layered structures showed good cell viability and effective osteoinductive and barrier properties. These results suggest that the novel multi‑layered PCL‑Gt/PCL‑simvastatin membranes have great potential for bone tissue engineering.
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July-2021
Volume 22 Issue 1

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Copy and paste a formatted citation
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Spandidos Publications style
Yu D, Huang C, Jiang C and Zhu H: Features of a simvastatin‑loaded multi‑layered co‑electrospun barrier membrane for guided bone regeneration. Exp Ther Med 22: 713, 2021
APA
Yu, D., Huang, C., Jiang, C., & Zhu, H. (2021). Features of a simvastatin‑loaded multi‑layered co‑electrospun barrier membrane for guided bone regeneration. Experimental and Therapeutic Medicine, 22, 713. https://doi.org/10.3892/etm.2021.10145
MLA
Yu, D., Huang, C., Jiang, C., Zhu, H."Features of a simvastatin‑loaded multi‑layered co‑electrospun barrier membrane for guided bone regeneration". Experimental and Therapeutic Medicine 22.1 (2021): 713.
Chicago
Yu, D., Huang, C., Jiang, C., Zhu, H."Features of a simvastatin‑loaded multi‑layered co‑electrospun barrier membrane for guided bone regeneration". Experimental and Therapeutic Medicine 22, no. 1 (2021): 713. https://doi.org/10.3892/etm.2021.10145