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Maternal obesity accelerated non‑alcoholic fatty liver disease in offspring mice by reducing autophagy

  • Authors:
    • Shuguang Han
    • Feng Zhu
    • Xiaoxia Huang
    • Panpan Yan
    • Ke Xu
    • Fangfang  Shen
    • Jiawen Sun
    • Zeyu Yang
    • Guoxi Jin
    • Yiqun Teng
  • View Affiliations / Copyright

    Affiliations: Graduate School, Bengbu Medical College, Bengbu, Anhui 233000, P.R. China, Department of Pediatrics, The Second Affiliated Hospital of Jiaxing University, Jiaxing Second Hospital, Jiaxing, Zhejiang 314000, P.R. China
    Copyright: © Han et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 716
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    Published online on: May 3, 2021
       https://doi.org/10.3892/etm.2021.10148
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Abstract

Non‑alcoholic fatty liver disease (NAFLD) is a chronic liver disease characterized by an excessive accumulation of triacylglycerol in the liver. Autophagy is a lysosome‑dependent degradation product recovery process, which widely occurs in eukaryotic cells, responsible for the vital maintenance of cellular energy balance. Previously published studies have demonstrated that autophagy is closely related to NAFLD occurrence and maternal obesity increases the susceptibility of offspring to non‑alcoholic fatty liver disease, however, the underlying mechanism of this remains unclear. In the present study, NAFLD mouse models (offspring of an obese mother mouse via high‑fat feeding) were generated, and the physiological indices of the liver were observed using total cholesterol, triglyceride, high‑density lipoprotein and low‑density lipoprotein serum assay kits. The morphological changes of the liver were also observed via HE, Masson and oil red O staining. Reverse transcription‑quantitative‑PCR and western blotting were performed to detect changes of autophagy‑related genes in liver or fibrosis marker proteins (α‑smooth muscle actin or TGF‑β1). Changes in serum inflammatory cytokine IL‑6 levels were determined via ELISA. The results of the present study demonstrated that the offspring of an obese mother were more likely to develop NALFD than the offspring of a chow‑fed mother, due to their increased association with liver fibrosis. When feeding continued to 17 weeks, the worst cases of NAFLD were observed and the level of autophagy decreased significantly compared with the offspring of a normal weight mouse. In addition, after 17 weeks of feeding, compared with the offspring of a chow‑fed mother, the offspring of an obese mouse mother had reduced adenosine 5'‑monophosphate (AMP)‑activated protein kinase (AMPK) phosphorylation levels and increased mammalian target of rapamycin (mTOR) phosphorylation levels. These results suggested that a reduced level of AMPK/mTOR mediated autophagy may be of vital importance for the increased susceptibility of offspring to NAFLD caused by maternal obesity. In conclusion, the current study provided a new direction for the treatment of NAFLD in offspring caused by maternal obesity.
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Copy and paste a formatted citation
Spandidos Publications style
Han S, Zhu F, Huang X, Yan P, Xu K, Shen F, Sun J, Yang Z, Jin G, Teng Y, Teng Y, et al: Maternal obesity accelerated non‑alcoholic fatty liver disease in offspring mice by reducing autophagy. Exp Ther Med 22: 716, 2021.
APA
Han, S., Zhu, F., Huang, X., Yan, P., Xu, K., Shen, F. ... Teng, Y. (2021). Maternal obesity accelerated non‑alcoholic fatty liver disease in offspring mice by reducing autophagy. Experimental and Therapeutic Medicine, 22, 716. https://doi.org/10.3892/etm.2021.10148
MLA
Han, S., Zhu, F., Huang, X., Yan, P., Xu, K., Shen, F., Sun, J., Yang, Z., Jin, G., Teng, Y."Maternal obesity accelerated non‑alcoholic fatty liver disease in offspring mice by reducing autophagy". Experimental and Therapeutic Medicine 22.1 (2021): 716.
Chicago
Han, S., Zhu, F., Huang, X., Yan, P., Xu, K., Shen, F., Sun, J., Yang, Z., Jin, G., Teng, Y."Maternal obesity accelerated non‑alcoholic fatty liver disease in offspring mice by reducing autophagy". Experimental and Therapeutic Medicine 22, no. 1 (2021): 716. https://doi.org/10.3892/etm.2021.10148
Copy and paste a formatted citation
x
Spandidos Publications style
Han S, Zhu F, Huang X, Yan P, Xu K, Shen F, Sun J, Yang Z, Jin G, Teng Y, Teng Y, et al: Maternal obesity accelerated non‑alcoholic fatty liver disease in offspring mice by reducing autophagy. Exp Ther Med 22: 716, 2021.
APA
Han, S., Zhu, F., Huang, X., Yan, P., Xu, K., Shen, F. ... Teng, Y. (2021). Maternal obesity accelerated non‑alcoholic fatty liver disease in offspring mice by reducing autophagy. Experimental and Therapeutic Medicine, 22, 716. https://doi.org/10.3892/etm.2021.10148
MLA
Han, S., Zhu, F., Huang, X., Yan, P., Xu, K., Shen, F., Sun, J., Yang, Z., Jin, G., Teng, Y."Maternal obesity accelerated non‑alcoholic fatty liver disease in offspring mice by reducing autophagy". Experimental and Therapeutic Medicine 22.1 (2021): 716.
Chicago
Han, S., Zhu, F., Huang, X., Yan, P., Xu, K., Shen, F., Sun, J., Yang, Z., Jin, G., Teng, Y."Maternal obesity accelerated non‑alcoholic fatty liver disease in offspring mice by reducing autophagy". Experimental and Therapeutic Medicine 22, no. 1 (2021): 716. https://doi.org/10.3892/etm.2021.10148
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