Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Experimental and Therapeutic Medicine
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-0981 Online ISSN: 1792-1015
Journal Cover
July-2021 Volume 22 Issue 1

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
July-2021 Volume 22 Issue 1

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML

  • Supplementary Files
    • Supplementary_Data.pdf
Article

Chrysoeriol ameliorates COX-2 expression through NF-κB, AP-1 and MAPK regulation via the TLR4/MyD88 signaling pathway in LPS-stimulated murine macrophages

  • Authors:
    • Hyun-Seo Yoon
    • Chung Mu Park
  • View Affiliations / Copyright

    Affiliations: Department of Dental Hygiene, Dong‑Eui University, Busanjin‑gu, Busan 47340, Republic of Korea, The Research Institute for Health Functional Materials, Dong‑Eui University, Busanjin‑gu, Busan 47340, Republic of Korea
  • Article Number: 718
    |
    Published online on: May 3, 2021
       https://doi.org/10.3892/etm.2021.10150
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Chrysoeriol is a flavonoid that has diverse biological properties, including antioxidation, anti‑inflammation, chemoprevention and immunomodulation. Despite its reported anti‑inflammatory activity, the exact underlying molecular mechanism has not yet been elucidated. In the current study, the anti‑inflammatory mechanism of chrysoeriol involving lipopolysaccharide (LPS)‑induced cyclooxygenase‑2 (COX-2) and its upstream signaling molecules was investigated in RAW 264.7 cells. The mechanism was evaluated via ELISA and western blotting assays. Chrysoeriol significantly inhibited LPS‑induced prostaglandin E2 (PGE2) production and COX‑2 expression without cytotoxicity. Activated transcription factors that further induced the inflammation response, including nuclear factor (NF)‑κB and activator protein‑1 (AP‑1), were significantly attenuated by chrysoeriol treatment. Furthermore, LPS‑induced phosphorylation levels of phosphoinositide‑3‑kinase (PI3K)/Akt and mitogen‑activated protein kinase (MAPK) were abolished by chrysoeriol treatment, which was confirmed by selective inhibitors. Additionally, chrysoeriol significantly inhibited the LPS‑induced activation of adaptor molecules in RAW 264.7 cells, including toll‑like receptor 4 (TLR4) and myeloid differentiation primary response 88. Therefore, the results suggested that chrysoeriol ameliorates TLR4‑mediated inflammatory responses by inhibiting NF‑κB and AP‑1 activation as well as suppressing PI3K/Akt and MAPK phosphorylation in LPS‑stimulated RAW 264.7 cells.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

View References

1 

Kunnumakkara AB, Sailo BL, Banik K, Harsha C, Prasad S, Gupta SC, Bharti AC and Aggarwal BB: Chronic diseases, inflammation, and spices: How are they linked? J Transl Med. 16(14)2018.PubMed/NCBI View Article : Google Scholar

2 

Kundu JK and Surh YJ: Inflammation: Gearing the journey to cancer. Mutat Res. 659:15–30. 2008.PubMed/NCBI View Article : Google Scholar

3 

Sukketsiri W, Tanasawet S, Moolsap F, Tantisira MH, Hutamekalin P and Tipmanee V: ECa 233 suppresses LPS-induced proinflammatory responses in macrophages via suppressing ERK1/2, p38 MAPK and akt pathways. Biol Pharm Bull. 42:1358–1365. 2019.PubMed/NCBI View Article : Google Scholar

4 

Park SA, Kim EH, Na HK and Surh YJ: KG-135 inhibits COX-2 expression by blocking the activation of JNK and AP-1 in phorbol ester-stimulated human breast epithelial cells. Ann NY Acad Sci. 1095:545–553. 2007.PubMed/NCBI View Article : Google Scholar

5 

Na HK and Surh YJ: Intracellular signaling network as a prime chemopreventive target of (-)-epigallocatechin gallate. Mol Nutr Food Res. 50:152–159. 2006.PubMed/NCBI View Article : Google Scholar

6 

Kim JH, Na HK, Pak YK, Lee YS, Lee SJ, Moon A and Surh YJ: Roles of ERK and p38 mitogen-activated protein kinases in phorbol ester-induced NF-kappaB activation and COX-2 expression in human breast epithelial cells. Chem Biol Interact. 171:133–141. 2008.PubMed/NCBI View Article : Google Scholar

7 

Kawamori T, Rao CV, Seibert K and Reddy BS: Chemopreventive activity of celecoxib, a specific cyclooxygenase-2 inhibitor, against colon carcinogenesis. Cancer Res. 58:409–412. 1998.PubMed/NCBI

8 

Choi CW, Jung HA, Kang SS and Choi JS: Antioxidant constituents and a new triterpenoid glycoside from flos lonicerae. Arch Pharm Res. 30:1–7. 2007.PubMed/NCBI View Article : Google Scholar

9 

Schinella GR, Giner RM, Recio MC, Mordujovich de Buschiazzo P, Rios JL and Manez S: Anti-inflammatory effects of South American Tanacetum vulgare. J Pharm Pharmacol. 50:1069–1074. 1998.PubMed/NCBI View Article : Google Scholar

10 

Abu Zarga M, Qauasmeh R, Sabri S, Munsoor M and Abdalla S: Chemical constituents of artemisia arborescens and the effect of the aqueous extract on rat isolated smooth muscle. Planta Med. 61:242–245. 1995.PubMed/NCBI View Article : Google Scholar

11 

Han LK, Sumiyoshi M, Zheng YN, Okuda H and Kimura Y: Anti-obesity action of salix matsudana leaves (Part 2). Isolation of anti-obesity effectors from polyphenol fractions of salix matsudana. Phytother Res. 17:1195–1198. 2003.PubMed/NCBI View Article : Google Scholar

12 

Mishra B, Priyadarsini KI, Kumar MS, Unnikrishnan MK and Mohan H: Effect of O-glycosilation on the antioxidant activity and free radical reactions of a plant flavonoid, chrysoeriol. Bioorg Med Chem. 11:2677–2685. 2003.PubMed/NCBI View Article : Google Scholar

13 

Choi DY, Lee JY, Kim MR, Woo ER, Kim YG and Kang KW: Chrysoeriol potently inhibits the induction of nitric oxide synthase by blocking AP-1 activation. J Biomed Sci. 12:949–959. 2005.PubMed/NCBI View Article : Google Scholar

14 

Kim JH, Cho YH, Park SM, Lee KE, Lee JJ, Lee BC, Pyo HB, Song KS, Park HD and Yun YP: Antioxidants and inhibitor of matrix metalloproteinase-1 expression from leaves of Zostera marina L. Arch Pharm Res. 27:177–183. 2004.PubMed/NCBI View Article : Google Scholar

15 

Park CM and Song YS: Luteolin and luteolin-7-O-glucoside inhibit lipopolysaccharide-induced inflammatory responses through modulation of NF-kB/AP-1/PI3K-Akt signaling cascades in RAW 264.7 cells. Nutr Res Pract. 7:423–429. 2013.PubMed/NCBI View Article : Google Scholar

16 

Bak MJ, Truong VL, Kang HS, Jun M and Jeong WS: Anti-inflammatory effect of procyanidins from wild grape (Vitis amurensis) seeds in LPS-induced RAW 264.7 cells. Oxid Med Cell Longev. 2013(409321)2013.PubMed/NCBI View Article : Google Scholar

17 

Chun J, Choi RJ, Khan S, Lee DS, Kim YC, Nam YJ, Lee DU and Kim YS: Alantolactone suppresses inducible nitric oxide synthase and cyclooxygenase-2 expression by down-regulating NF-kB, MAPK and AP-1 via the MyD88 signaling pathway in LPS-activated RAW 264.7 cells. Int Immunopharmacol. 14:375–383. 2012.PubMed/NCBI View Article : Google Scholar

18 

Jeong D, Dong GZ, Lee HJ and Ryu JH: Anti-inflammatory compounds from atractylodes macrocephala. Molecules. 24(1859)2019.PubMed/NCBI View Article : Google Scholar

19 

Xie WL, Chipman JG, Robertson DL, Erikson RL and Simmons DL: Expression of a mitogen-responsive gene encoding prostaglandin synthase is regulated by mRNA splicing. Proc Natl Acad Sci USA. 88:2692–2696. 1991.PubMed/NCBI View Article : Google Scholar

20 

Yang Y, Zhou X, Xiao M, Hong Z, Gong Q, Jiang L and Zhou J: Discovery of chrysoeriol, a PI3K-AKT-mTOR pathway inhibitor with potent antitumor activity against human multiple myeloma cells in vitro. J Huazhong Univ Sci Technolog Med Sci. 30:734–740. 2010.PubMed/NCBI View Article : Google Scholar

21 

Medzhitov R: Origin and physiological roles of inflammation. Nature. 454:428–435. 2008.PubMed/NCBI View Article : Google Scholar

22 

Tabas I and Glass CK: Anti-inflammatory therapy in chronic disease: Challenges and opportunities. Science. 339:166–172. 2013.PubMed/NCBI View Article : Google Scholar

23 

Qian C, Liu J and Cao X: Innate signaling in the inflammatory immune disorders. Cytokine Growth Factor Rev. 25:731–738. 2014.PubMed/NCBI View Article : Google Scholar

24 

Schindler SM, Little JP and Klegeris A: Microparticles: A new perspective in central nervous system disorders. Biomed Res Int. 2014(756327)2014.PubMed/NCBI View Article : Google Scholar

25 

Van Dyke TE and van Winkelhoff AJ: Infection and inflammatory mechanisms. J Periodontol. 84 (Suppl 4):S1–S7. 2013.PubMed/NCBI View Article : Google Scholar

26 

Meng F and Lowell CA: Lipopolysaccharide (LPS)-induced macrophage activation and signal transduction in the absence of Src-family kinases Hck, Fgr, and Lyn. J Exp Med. 185:1661–1670. 1997.PubMed/NCBI View Article : Google Scholar

27 

Roy A, Srivastava M, Saqib U, Liu D, Faisal SM, Sugathan S, Bishnoi S and Baig MS: Potential therapeutic targets for inflammation in toll-like receptor 4 (TLR4)-mediated signaling pathways. Int Immunopharmacol. 40:79–89. 2016.PubMed/NCBI View Article : Google Scholar

28 

Hu W, Wu L, Qiang Q, Ji L, Wang X, Luo H, Wu H, Jiang Y, Wang G and Shen T: The dichloromethane fraction from Mahonia bealei (Fort.) Carr. leaves exerts an anti-inflammatory effect both in vitro and in vivo. J Ethnopharmacol. 188:134–143. 2016.PubMed/NCBI View Article : Google Scholar

29 

Wang J, Kang YX, Pan W, Lei W, Feng B and Wang XJ: Enhancement of anti-inflammatory activity of curcumin using phosphatidylserine-containing nanoparticles in cultured macrophages. Int J Mol Sci. 17(969)2016.PubMed/NCBI View Article : Google Scholar

30 

Surh YJ: Cancer chemoprevention with dietary phytochemicals. Nat Rev Cancer. 3:768–780. 2003.PubMed/NCBI View Article : Google Scholar

31 

Endale M, Park SC, Kim S, Kim SH, Yang Y, Cho JY and Rhee MH: Quercetin disrupts tyrosine-phosphorylated phosphatidylinositol 3-kinase and myeloid differentiation factor-88 association, and inhibits MAPK/AP-1 and IKK/NF-kB-induced inflammatory mediators production in RAW 264.7 cells. Immunobiology. 218:1452–1467. 2013.PubMed/NCBI View Article : Google Scholar

32 

Chen K, Huang J, Gong W, Iribarren P, Dunlop NM and Wang JM: Toll-like receptors in inflammation, infection and cancer. Int Immunopharmacol. 7:1271–1285. 2007.PubMed/NCBI View Article : Google Scholar

33 

Dauphinee SM and Karsan A: Lipopolysaccharide signaling in endothelial cells. Lab Invest. 86:9–22. 2006.PubMed/NCBI View Article : Google Scholar

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Yoon H and Park CM: Chrysoeriol ameliorates COX-2 expression through NF-κB, AP-1 and MAPK regulation via the TLR4/MyD88 signaling pathway in LPS-stimulated murine macrophages. Exp Ther Med 22: 718, 2021.
APA
Yoon, H., & Park, C.M. (2021). Chrysoeriol ameliorates COX-2 expression through NF-κB, AP-1 and MAPK regulation via the TLR4/MyD88 signaling pathway in LPS-stimulated murine macrophages. Experimental and Therapeutic Medicine, 22, 718. https://doi.org/10.3892/etm.2021.10150
MLA
Yoon, H., Park, C. M."Chrysoeriol ameliorates COX-2 expression through NF-κB, AP-1 and MAPK regulation via the TLR4/MyD88 signaling pathway in LPS-stimulated murine macrophages". Experimental and Therapeutic Medicine 22.1 (2021): 718.
Chicago
Yoon, H., Park, C. M."Chrysoeriol ameliorates COX-2 expression through NF-κB, AP-1 and MAPK regulation via the TLR4/MyD88 signaling pathway in LPS-stimulated murine macrophages". Experimental and Therapeutic Medicine 22, no. 1 (2021): 718. https://doi.org/10.3892/etm.2021.10150
Copy and paste a formatted citation
x
Spandidos Publications style
Yoon H and Park CM: Chrysoeriol ameliorates COX-2 expression through NF-κB, AP-1 and MAPK regulation via the TLR4/MyD88 signaling pathway in LPS-stimulated murine macrophages. Exp Ther Med 22: 718, 2021.
APA
Yoon, H., & Park, C.M. (2021). Chrysoeriol ameliorates COX-2 expression through NF-κB, AP-1 and MAPK regulation via the TLR4/MyD88 signaling pathway in LPS-stimulated murine macrophages. Experimental and Therapeutic Medicine, 22, 718. https://doi.org/10.3892/etm.2021.10150
MLA
Yoon, H., Park, C. M."Chrysoeriol ameliorates COX-2 expression through NF-κB, AP-1 and MAPK regulation via the TLR4/MyD88 signaling pathway in LPS-stimulated murine macrophages". Experimental and Therapeutic Medicine 22.1 (2021): 718.
Chicago
Yoon, H., Park, C. M."Chrysoeriol ameliorates COX-2 expression through NF-κB, AP-1 and MAPK regulation via the TLR4/MyD88 signaling pathway in LPS-stimulated murine macrophages". Experimental and Therapeutic Medicine 22, no. 1 (2021): 718. https://doi.org/10.3892/etm.2021.10150
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team