Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Experimental and Therapeutic Medicine
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-0981 Online ISSN: 1792-1015
Journal Cover
July-2021 Volume 22 Issue 1

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
July-2021 Volume 22 Issue 1

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article Open Access

MicroRNA‑124‑3p inhibits the differentiation of precartilaginous stem cells into nucleus pulposus‑like cells via targeting FSTL1

  • Authors:
    • Qiong Wang
    • Junfang Wang
    • Xiaofeng Gu
    • Dehong Feng
    • Ding Li
    • Tao Jiang
  • View Affiliations / Copyright

    Affiliations: Department of Clinical Laboratory, Wuxi People's Hospital Affiliated to Nanjing Medical University, Wuxi, Jiangsu 214000, P.R. China, Department of Orthopedics, Wuxi People's Hospital Affiliated to Nanjing Medical University, Wuxi, Jiangsu 214000, P.R. China
    Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 725
    |
    Published online on: May 4, 2021
       https://doi.org/10.3892/etm.2021.10157
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

MicroRNA (miRNA/miR)‑124‑3p has been extensively studied in tumor biology and stem cells. However, little is known regarding its functional roles in the differentiation of precartilaginous stem cells (PSCs) into nucleus pulposus‑like cells (NPLCs). In the present study, using miRNA microarray screening, it was demonstrated that the miRNA expression profiles differed between rat primary PSCs and TGF‑β1‑induced differentiated NPLCs, and that miR‑124‑3p was significantly differentially expressed during the differentiation of PSCs to NPLCs. Furthermore, RT‑qPCR analysis verified that miR‑124‑3p expression was decreased during PSC differentiation, with the lowest levels being detected at the later stages. Subsequent experiments revealed that miR‑124‑3p overexpression significantly decreased the expression of the extracellular matrix proteins, aggrecan and collagen type II, which was accompanied by a significant decrease in follistatin‑related protein 1 (FSTL1) expression levels. Moreover, bioinformatics analysis indicated that FSTL1 was a potential target of miR‑124‑3p, which was additionally verified using luciferase reporter assays. Taken together, these data revealed a specific regulatory pathway of miR‑124‑3p, which negatively regulated its target gene, FSTL1, during the differentiation of PSCs to NPLCs, and suggested a functional role for miR‑124‑3p in the differentiation of PSCs.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

View References

1 

Fardon DF, Williams AL, Dohring EJ, Murtagh FR, Gabriel Rothman SL and Sze GK: Lumbar disc nomenclature: Version 2.0: Recommendations of the combined task forces of the North American Spine Society, the American Society of Spine Radiology, and the American Society of Neuroradiology. Spine J. 14:2525–2545. 2014.PubMed/NCBI View Article : Google Scholar

2 

Hemanta D, Jiang XX, Feng ZZ, Chen ZX and Cao YW: Etiology for Degenerative Disc Disease. Chin Med Sci J. 31:185–191. 2016.PubMed/NCBI View Article : Google Scholar

3 

Lu H and Peng L: Efficacy and safety of Mobi-C cervical artificial disc versus anterior discectomy and fusion in patients with symptomatic degenerative disc disease: A meta-analysis. Medicine (Baltimore). 96(e8504)2017.PubMed/NCBI View Article : Google Scholar

4 

Matta A, Karim MZ, Isenman DE and Erwin WM: Molecular therapy for degenerative disc disease: Clues from secretome analysis of the notochordal cell-rich nucleus pulposus. Sci Rep. 7(45623)2017.PubMed/NCBI View Article : Google Scholar

5 

Liang L, Li X, Li D, Jiang W, Wang H, Chen J, Sun Z, Zhang N and Zhu Y: The characteristics of stem cells in human degenerative intervertebral disc. Medicine (Baltimore). 96(e7178)2017.PubMed/NCBI View Article : Google Scholar

6 

Guo J, Shao M, Lu F, Jiang J and Xia X: Role of Sirt1 plays in nucleus pulposus cells and intervertebral disc degeneration. Spine (Phila Pa 1976). 42:E757–E766. 2017.PubMed/NCBI View Article : Google Scholar

7 

Vedicherla S and Buckley CT: Cell-based therapies for intervertebral disc and cartilage regeneration - Current concepts, parallels, and perspectives. J Orthop Res. 35:8–22. 2017.PubMed/NCBI View Article : Google Scholar

8 

Skovrlj B, Cunn G, Guzman JZ and Qureshi SA: Mesenchymal stem cell technology in the treatment of degenerative disc disease. J Neurosurg Sci. 59:25–35. 2015.PubMed/NCBI

9 

Centeno C, Markle J, Dodson E, Stemper I, Williams CJ, Hyzy M, Ichim T and Freeman M: Treatment of lumbar degenerative disc disease-associated radicular pain with culture-expanded autologous mesenchymal stem cells: A pilot study on safety and efficacy. J Transl Med. 15(197)2017.PubMed/NCBI View Article : Google Scholar

10 

Li XC, Tang Y, Wu JH, Yang PS, Wang DL and Ruan DK: Characteristics and potentials of stem cells derived from human degenerated nucleus pulposus: Potential for regeneration of the intervertebral disc. BMC Musculoskelet Disord. 18(242)2017.PubMed/NCBI View Article : Google Scholar

11 

Zhang S, Chen A, Hu W, Li M, Liao H, Zhu W, Song D and Guo F: Immunological purification of rat precartilaginous stem cells and construction of the immortalized cell strain. Arch Orthop Trauma Surg. 128:1339–1344. 2008.PubMed/NCBI View Article : Google Scholar

12 

Li C, Wang Q and Wang JF: Transforming growth factor-β (TGF-β) induces the expression of chondrogenesis-related genes through TGF-β receptor II (TGFRII)-AKT-mTOR signaling in primary cultured mouse precartilaginous stem cells. Biochem Biophys Res Commun. 450:646–651. 2014.PubMed/NCBI View Article : Google Scholar

13 

Fan MP, Si M, Li BJ, Hu GH, Hou Y, Yang W, Liu L, Tang B and Nie L: Cell therapy of a knee osteoarthritis rat model using precartilaginous stem cells. Eur Rev Med Pharmacol Sci. 22:2119–2125. 2018.PubMed/NCBI View Article : Google Scholar

14 

Richardson SM, Kalamegam G, Pushparaj PN, Matta C, Memic A, Khademhosseini A, Mobasheri R, Poletti FL, Hoyland JA and Mobasheri A: Mesenchymal stem cells in regenerative medicine: Focus on articular cartilage and intervertebral disc regeneration. Methods. 99:69–80. 2016.PubMed/NCBI View Article : Google Scholar

15 

Xia K, Gong Z, Zhu J, Yu W, Wang Y, Wang J, Xu A, Zhou X, Tao H, Li F and Liang C: Differentiation of pluripotent stem cells into nucleus pulposus progenitor cells for intervertebral disc regeneration. Curr Stem Cell Res Ther. 14:57–64. 2019.PubMed/NCBI View Article : Google Scholar

16 

Chen P, Ning L, Qiu P, Mo J, Mei S, Xia C, Zhang J, Lin X and Fan S: Photo-crosslinked gelatin-hyaluronic acid methacrylate hydrogel-committed nucleus pulposus-like differentiation of adipose stromal cells for intervertebral disc repair. J Tissue Eng Regen Med. 13:682–693. 2019.PubMed/NCBI View Article : Google Scholar

17 

Ma K, Chen S, Li Z, Deng X, Huang D, Xiong L and Shao Z: Mechanisms of endogenous repair failure during intervertebral disc degeneration. Osteoarthritis Cartilage. 27:41–48. 2019.PubMed/NCBI View Article : Google Scholar

18 

Yang Z, Gao XJ and Zhao X: CDMP1 promotes type II collagen and aggrecan synthesis of nucleus pulposus cell via the mediation of ALK6. Eur Rev Med Pharmacol Sci. 24:10975–10983. 2020.PubMed/NCBI View Article : Google Scholar

19 

Le Maitre CL, Pockert A, Buttle DJ, Freemont AJ and Hoyland JA: Matrix synthesis and degradation in human intervertebral disc degeneration. Biochem Soc Trans. 35:652–655. 2007.PubMed/NCBI View Article : Google Scholar

20 

Yang SH, Wu CC, Shih TT, Sun YH and Lin FH: In vitro study on interaction between human nucleus pulposus cells and mesenchymal stem cells through paracrine stimulation. Spine (Phila Pa 1976). 33:1951–1957. 2008.PubMed/NCBI View Article : Google Scholar

21 

Wang Q, Gu X, Cheng L and Wang J: Differentiation of immortalized human precartilaginous stem cells into nucleus pulposus-like cells. Int J Clin Exp Pathol. 8:2816–2822. 2015.PubMed/NCBI

22 

Saliminejad K, Khorram Khorshid HR, Soleymani Fard S and Ghaffari SH: An overview of microRNAs: Biology, functions, therapeutics, and analysis methods. J Cell Physiol. 234:5451–5465. 2019.PubMed/NCBI View Article : Google Scholar

23 

Wang C, Wang WJ, Yan YG, Xiang YX, Zhang J, Tang ZH and Jiang ZS: MicroRNAs: New players in intervertebral disc degeneration. Clin Chim Acta. 450:333–341. 2015.PubMed/NCBI View Article : Google Scholar

24 

Wang Q, Gu X and Wang J: Transforming growth factor-β1 (TGF-β1) induces mouse precartilaginous stem cell differentiation through TGFRII-CK1ε-β-catenin signalling. Int J Exp Pathol. 99:113–120. 2018.PubMed/NCBI View Article : Google Scholar

25 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001.PubMed/NCBI View Article : Google Scholar

26 

Zhou X, Tao Y, Chen E, Wang J, Fang W, Zhao T, Liang C, Li F and Chen Q: Genipin-cross-linked type II collagen scaffold promotes the differentiation of adipose-derived stem cells into nucleus pulposus-like cells. J Biomed Mater Res A. 106:1258–1268. 2018.PubMed/NCBI View Article : Google Scholar

27 

Vishnoi A and Rani S: miRNA biogenesis and regulation of diseases: An overview. Methods Mol Biol. 1509:1–10. 2017.PubMed/NCBI View Article : Google Scholar

28 

Weber KT, Jacobsen TD, Maidhof R, Virojanapa J, Overby C, Bloom O, Quraishi S, Levine M and Chahine NO: Developments in intervertebral disc disease research: Pathophysiology, mechanobiology, and therapeutics. Curr Rev Musculoskelet Med. 8:18–31. 2015.PubMed/NCBI View Article : Google Scholar

29 

Kang Q, Xiang Y, Li D, Liang J, Zhang X, Zhou F, Qiao M, Nie Y, He Y, Cheng J, et al: miR-124-3p attenuates hyperphosphorylation of Tau protein-induced apoptosis via caveolin-1-PI3K/Akt/GSK3β pathway in N2a/APP695swe cells. Oncotarget. 8:24314–24326. 2017.PubMed/NCBI View Article : Google Scholar

30 

Zo RB and Long Z: miR-124-3p suppresses bladder cancer by targeting DNA methyltransferase 3B. J Cell Physiol. 234:464–474. 2018.PubMed/NCBI View Article : Google Scholar

31 

Long HD, Ma YS, Yang HQ, Xue SB, Liu JB, Yu F, Lv ZW, Li JY, Xie RT, Chang ZY, et al: Reduced hsa-miR-124-3p levels are associated with the poor survival of patients with hepatocellular carcinoma. Mol Biol Rep. 45:2615–2623. 2018.PubMed/NCBI View Article : Google Scholar

32 

Luo L, Chi H and Ling J: miR-124-3p suppresses glioma aggressiveness via targeting of Fra-2. Pathol Res Pract. 214:1825–1834. 2018.PubMed/NCBI View Article : Google Scholar

33 

Zhang Y, Liu HL, An LJ, Li L, Wei M, Ge DJ and Su Z: miR-124-3p attenuates neuropathic pain induced by chronic sciatic nerve injury in rats via targeting EZH2. J Cell Biochem. 120:5747–5755. 2019.PubMed/NCBI View Article : Google Scholar

34 

Tao Y, Zhou X, Liu D, Li H, Liang C, Li F and Chen Q: Proportion of collagen type II in the extracellular matrix promotes the differentiation of human adipose-derived mesenchymal stem cells into nucleus pulposus cells. Biofactors. 42:212–223. 2016.PubMed/NCBI View Article : Google Scholar

35 

Lian C, Gao B, Wu Z, Qiu X, Peng Y, Liang A, Xu C, Su P and Huang D: Collagen type II is downregulated in the degenerative nucleus pulposus and contributes to the degeneration and apoptosis of human nucleus pulposus cells. Mol Med Rep. 16:4730–4736. 2017.PubMed/NCBI View Article : Google Scholar

36 

Shibanuma M, Mashimo J, Mita A, Kuroki T and Nose K: Cloning from a mouse osteoblastic cell line of a set of transforming-growth-factor-beta 1-regulated genes, one of which seems to encode a follistatin-related polypeptide. Eur J Biochem. 217:13–19. 1993.PubMed/NCBI View Article : Google Scholar

37 

Ogura Y, Ouchi N, Ohashi K, Shibata R, Kataoka Y, Kambara T, Kito T, Maruyama S, Yuasa D, Matsuo K, et al: Therapeutic impact of follistatin-like 1 on myocardial ischemic injury in preclinical models. Circulation. 126:1728–1738. 2012.PubMed/NCBI View Article : Google Scholar

38 

Torres S, Bartolomé RA, Mendes M, Barderas R, Fernandez-Aceñero MJ, Peláez-García A, Peña C, Lopez-Lucendo M, Villar-Vázquez R, de Herreros AG, et al: Proteome profiling of cancer-associated fibroblasts identifies novel proinflammatory signatures and prognostic markers for colorectal cancer. Clin Cancer Res. 19:6006–6019. 2013.PubMed/NCBI View Article : Google Scholar

39 

Wilson DC, Marinov AD, Blair HC, Bushnell DS, Thompson SD, Chaly Y and Hirsch R: Follistatin-like protein 1 is a mesenchyme-derived inflammatory protein and may represent a biomarker for systemic-onset juvenile rheumatoid arthritis. Arthritis Rheumatism. 62:2510–2516. 2010.PubMed/NCBI View Article : Google Scholar

40 

Liu Y, Wei J, Zhao Y, Zhang Y, Han Y, Chen B, Cheng K, Jia J, Nie L and Cheng L: Follistatin-like protein 1 promotes inflammatory reactions in nucleus pulposus cells by interacting with the MAPK and NFκB signaling pathways. Oncotarget. 8:43023–43034. 2017.PubMed/NCBI View Article : Google Scholar

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Wang Q, Wang J, Gu X, Feng D, Li D and Jiang T: MicroRNA‑124‑3p inhibits the differentiation of precartilaginous stem cells into nucleus pulposus‑like cells via targeting FSTL1. Exp Ther Med 22: 725, 2021.
APA
Wang, Q., Wang, J., Gu, X., Feng, D., Li, D., & Jiang, T. (2021). MicroRNA‑124‑3p inhibits the differentiation of precartilaginous stem cells into nucleus pulposus‑like cells via targeting FSTL1. Experimental and Therapeutic Medicine, 22, 725. https://doi.org/10.3892/etm.2021.10157
MLA
Wang, Q., Wang, J., Gu, X., Feng, D., Li, D., Jiang, T."MicroRNA‑124‑3p inhibits the differentiation of precartilaginous stem cells into nucleus pulposus‑like cells via targeting FSTL1". Experimental and Therapeutic Medicine 22.1 (2021): 725.
Chicago
Wang, Q., Wang, J., Gu, X., Feng, D., Li, D., Jiang, T."MicroRNA‑124‑3p inhibits the differentiation of precartilaginous stem cells into nucleus pulposus‑like cells via targeting FSTL1". Experimental and Therapeutic Medicine 22, no. 1 (2021): 725. https://doi.org/10.3892/etm.2021.10157
Copy and paste a formatted citation
x
Spandidos Publications style
Wang Q, Wang J, Gu X, Feng D, Li D and Jiang T: MicroRNA‑124‑3p inhibits the differentiation of precartilaginous stem cells into nucleus pulposus‑like cells via targeting FSTL1. Exp Ther Med 22: 725, 2021.
APA
Wang, Q., Wang, J., Gu, X., Feng, D., Li, D., & Jiang, T. (2021). MicroRNA‑124‑3p inhibits the differentiation of precartilaginous stem cells into nucleus pulposus‑like cells via targeting FSTL1. Experimental and Therapeutic Medicine, 22, 725. https://doi.org/10.3892/etm.2021.10157
MLA
Wang, Q., Wang, J., Gu, X., Feng, D., Li, D., Jiang, T."MicroRNA‑124‑3p inhibits the differentiation of precartilaginous stem cells into nucleus pulposus‑like cells via targeting FSTL1". Experimental and Therapeutic Medicine 22.1 (2021): 725.
Chicago
Wang, Q., Wang, J., Gu, X., Feng, D., Li, D., Jiang, T."MicroRNA‑124‑3p inhibits the differentiation of precartilaginous stem cells into nucleus pulposus‑like cells via targeting FSTL1". Experimental and Therapeutic Medicine 22, no. 1 (2021): 725. https://doi.org/10.3892/etm.2021.10157
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team