Open Access

Potential protective effect of hesperidin on hypoxia/reoxygenation‑induced hepatocyte injury

  • Authors:
    • Shilai Li
    • Jijin Zhu
    • Ling Pan
    • Peiqi Wan
    • Quanlin Qin
    • Daqing Luo
    • Wenhui Pan
    • Yuqing Wei
    • Yansong Xu
    • Liming Shang
    • Xinping Ye
  • View Affiliations

  • Published online on: May 13, 2021     https://doi.org/10.3892/etm.2021.10196
  • Article Number: 764
  • Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Hesperidin (HDN) has been reported to have hydrogen radical‑ and hydrogen peroxide‑removal activities and to serve an antioxidant role in biological systems. However, whether HDN protects hepatocytes (HCs) against hypoxia/reoxygenation (H/R)‑induced injury remains unknown. The present study aimed to explore the role of HDN in H/R‑induced injury. HCs were isolated and cultured under H/R conditions with or without HDN treatment. HC damage was markedly induced under H/R, as indicated by cell viability, supernatant lactate dehydrogenase levels and alanine aminotransferase levels; however, HDN treatment significantly reversed HC injury. Oxidative stress markers (malondialdehyde, superoxide dismutase, glutathioneand reactive oxygen species) were increased markedly during H/R in HCs; however, this effect was significantly attenuated after exposure to HDN. Compared with those of the control group, the mRNA expression levels of IL‑6 and TNF‑α in HCs and the concentrations of IL‑6 and TNF‑α in the supernatants increased significantly following H/R, and HDN significantly ameliorated these effects. Western blotting demonstrated that microtubule‑associated protein 1 light chain 3α (MAP1LC3A, also known as LC3) and Beclin‑1 protein expression levels increased, while sequestosome 1 levels decreased during H/R following exposure to HDN. The number of GFP‑LC3 puncta in HCs following exposure to HDN was increased compared with that observed in HCs without HDN exposure under the H/R conditions after bafilomycin A1 treatment. In summary, the present study demonstrated that HDN attenuated HC oxidative stress and inflammatory responses while enhancing autophagy during H/R. HDN may have a potential protective effect on HCs during H/R‑induced injury.
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July-2021
Volume 22 Issue 1

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Li S, Zhu J, Pan L, Wan P, Qin Q, Luo D, Pan W, Wei Y, Xu Y, Shang L, Shang L, et al: Potential protective effect of hesperidin on hypoxia/reoxygenation‑induced hepatocyte injury. Exp Ther Med 22: 764, 2021
APA
Li, S., Zhu, J., Pan, L., Wan, P., Qin, Q., Luo, D. ... Ye, X. (2021). Potential protective effect of hesperidin on hypoxia/reoxygenation‑induced hepatocyte injury. Experimental and Therapeutic Medicine, 22, 764. https://doi.org/10.3892/etm.2021.10196
MLA
Li, S., Zhu, J., Pan, L., Wan, P., Qin, Q., Luo, D., Pan, W., Wei, Y., Xu, Y., Shang, L., Ye, X."Potential protective effect of hesperidin on hypoxia/reoxygenation‑induced hepatocyte injury". Experimental and Therapeutic Medicine 22.1 (2021): 764.
Chicago
Li, S., Zhu, J., Pan, L., Wan, P., Qin, Q., Luo, D., Pan, W., Wei, Y., Xu, Y., Shang, L., Ye, X."Potential protective effect of hesperidin on hypoxia/reoxygenation‑induced hepatocyte injury". Experimental and Therapeutic Medicine 22, no. 1 (2021): 764. https://doi.org/10.3892/etm.2021.10196