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Diabetic neuropathic pain induced by streptozotocin alters the expression profile of non‑coding RNAs in the spinal cord of mice as determined by sequencing analysis

  • Authors:
    • Jian He
    • Han Bin Wang
    • Jiang Ju Huang
    • Lei Zhang
    • Dong Lin Li
    • Wan You He
    • Qing Ming Xiong
    • Zai Sheng Qin
  • View Affiliations / Copyright

    Affiliations: Department of Anesthesiology, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong 510000, P.R. China, Department of Anesthesiology, The First People's Hospital of Foshan, Foshan, Guangdong 528000, P.R. China
    Copyright: © He et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 775
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    Published online on: May 18, 2021
       https://doi.org/10.3892/etm.2021.10207
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Abstract

Diabetic neuropathic pain (DNP) is one of the most serious complications of diabetes. Patients with DNP always exhibit spontaneous and stimulus‑evoked pain. However, the pathogenesis of DNP remains to be fully elucidated. Non‑coding RNAs (ncRNAs) serve important roles in several cellular processes and dysregulated expression may result in the development of several diseases, including DNP. Although ncRNAs have been suggested to be involved in the pathogenesis of DNP, their precise roles remain to be determined. In the present study, sequencing analysis was used to investigate the expression patterns of coding genes, microRNAs (miRNAs), long ncRNAs (lncRNAs) and circular RNAs (circRNAs) in the spinal cord of mice with streptozotocin (STZ)‑induced DNP. A total of 30 mRNAs, 148 miRNAs, 9 lncRNAs and 135 circRNAs exhibited significantly dysregulated expression 42 days after STZ injection. Functional enrichment analysis indicated that protein digestion and absorption pathways were the most significantly affected pathways of the differentially expressed (DE) mRNAs. The Rap1 signaling pathway, human T‑lymphotropic virus‑I infection and the MAPK signaling pathway were the three most significant pathways of the DE miRNAs. A total of 2,118 distinct circRNAs were identified and the length of the majority of the circRNAs was <1,000 nucleotides (nt) (1,552 circRNAs were >1,000 nt) with a median length of 620 nt. In the present study, the expression characteristics of coding genes, miRNAs, lncRNAs and circRNAs in DNP mice were determined; it paves the road for further studies on the mechanisms associated with DNP and potentially facilitates the discovery of novel ncRNAs for therapeutic targeting in the management of DNP.
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Copy and paste a formatted citation
Spandidos Publications style
He J, Wang HB, Huang JJ, Zhang L, Li DL, He WY, Xiong QM and Qin ZS: Diabetic neuropathic pain induced by streptozotocin alters the expression profile of non‑coding RNAs in the spinal cord of mice as determined by sequencing analysis. Exp Ther Med 22: 775, 2021.
APA
He, J., Wang, H.B., Huang, J.J., Zhang, L., Li, D.L., He, W.Y. ... Qin, Z.S. (2021). Diabetic neuropathic pain induced by streptozotocin alters the expression profile of non‑coding RNAs in the spinal cord of mice as determined by sequencing analysis. Experimental and Therapeutic Medicine, 22, 775. https://doi.org/10.3892/etm.2021.10207
MLA
He, J., Wang, H. B., Huang, J. J., Zhang, L., Li, D. L., He, W. Y., Xiong, Q. M., Qin, Z. S."Diabetic neuropathic pain induced by streptozotocin alters the expression profile of non‑coding RNAs in the spinal cord of mice as determined by sequencing analysis". Experimental and Therapeutic Medicine 22.1 (2021): 775.
Chicago
He, J., Wang, H. B., Huang, J. J., Zhang, L., Li, D. L., He, W. Y., Xiong, Q. M., Qin, Z. S."Diabetic neuropathic pain induced by streptozotocin alters the expression profile of non‑coding RNAs in the spinal cord of mice as determined by sequencing analysis". Experimental and Therapeutic Medicine 22, no. 1 (2021): 775. https://doi.org/10.3892/etm.2021.10207
Copy and paste a formatted citation
x
Spandidos Publications style
He J, Wang HB, Huang JJ, Zhang L, Li DL, He WY, Xiong QM and Qin ZS: Diabetic neuropathic pain induced by streptozotocin alters the expression profile of non‑coding RNAs in the spinal cord of mice as determined by sequencing analysis. Exp Ther Med 22: 775, 2021.
APA
He, J., Wang, H.B., Huang, J.J., Zhang, L., Li, D.L., He, W.Y. ... Qin, Z.S. (2021). Diabetic neuropathic pain induced by streptozotocin alters the expression profile of non‑coding RNAs in the spinal cord of mice as determined by sequencing analysis. Experimental and Therapeutic Medicine, 22, 775. https://doi.org/10.3892/etm.2021.10207
MLA
He, J., Wang, H. B., Huang, J. J., Zhang, L., Li, D. L., He, W. Y., Xiong, Q. M., Qin, Z. S."Diabetic neuropathic pain induced by streptozotocin alters the expression profile of non‑coding RNAs in the spinal cord of mice as determined by sequencing analysis". Experimental and Therapeutic Medicine 22.1 (2021): 775.
Chicago
He, J., Wang, H. B., Huang, J. J., Zhang, L., Li, D. L., He, W. Y., Xiong, Q. M., Qin, Z. S."Diabetic neuropathic pain induced by streptozotocin alters the expression profile of non‑coding RNAs in the spinal cord of mice as determined by sequencing analysis". Experimental and Therapeutic Medicine 22, no. 1 (2021): 775. https://doi.org/10.3892/etm.2021.10207
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