Combination of endothelial progenitor cells and BB‑94 significantly alleviates brain damage in a mouse model of diabetic ischemic stroke
- Daixuan Zhou
- Zhi Huang
- Xiaoxi Zhu
- Tao Hong
- Yuanli Zhao
Affiliations: Queen Mary College, Nanchang University, Nanchang, Jiangxi 330031, P.R. China, Key Laboratory of Endemic and Ethnic Diseases, Ministry of Education, Guizhou Medical University, Guiyang, Guizhou 550002, P.R. China, Department of Neurosurgery, The First Affiliated Hospital of Nanchang University, Nanchang, Jiangxi 330029, P.R. China, Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing 100070, P.R. China
- Published online on: May 21, 2021 https://doi.org/10.3892/etm.2021.10221
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Ischemic stroke is a complication of chronic macrovascular disease in type 2 diabetes. However, the pathogenesis of diabetic ischemic stroke has not yet been fully clarified. The aim of the present study was to investigate the underlying effects of endothelial progenitor cells (EPCs) and the matrix metalloproteinase inhibitor BB‑94 on diabetic stroke. In vitro experiments were performed using oxygen‑glucose deprivation/reoxygenation (OGD/R) model cells, established using HT22 mouse hippocampal cells. MTT assays and flow cytometry revealed that BB‑94 prominently induced the proliferation of the OGD/R model cells and prevented their apoptosis. When EPCs and BB‑94 were applied to the OGD/R model cells in combination, proliferation was further accelerated and oxidative damage was attenuated. In vivo experiments were also performed using a middle cerebral artery occlusion (MCAO) mouse model. The results of modified neurological severity scoring and oxidative stress marker analysis demonstrated that EPCs and BB‑94 prominently alleviated cerebral ischemia/reperfusion injury in the MCAO model mice. Furthermore, reverse transcription‑quantitative PCR and western blot assays revealed that EPCs in combination with BB‑94 significantly downregulated the expression of matrix metalloproteinases (MMPs) and upregulated the expression of tissue inhibitor of metalloproteinases 1 in OGD/R cells and MCAO model mice. The results suggest that EPCs were successfully isolated and identified, and the OGD/R cell and MCAO mouse models were successfully established. They also indicate that EPCs alone or in combination with BB‑94 may exert protective effects against ischemic stroke via the reduction of MMP expression.