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miR‑9 alleviated the inflammatory response and apoptosis in caerulein‑induced acute pancreatitis by regulating FGF10 and the NF‑κB signaling pathway

  • Authors:
    • Yang Shen
    • Chengjun Xue
    • Guoli You
    • Cui Liu
  • View Affiliations / Copyright

    Affiliations: Department of Gastroenterology, Jiangsu Hospital, Nantong University, Nantong, Jiangsu 224700, P.R. China
    Copyright: © Shen et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 795
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    Published online on: May 25, 2021
       https://doi.org/10.3892/etm.2021.10227
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Abstract

MicroRNAs (miRs) have been implicated in the development of acute pancreatitis (AP). However, the role and potential mechanism of miR‑9 in AP progression remains unclear. Caerulein‑treated AR42J cells were used as a cellular model of AP. Results revealed caerulein triggered an inflammatory response by promoting the secretion of inflammatory cytokines [tumor necrosis factor‑α, interleukin (IL) 1β and IL‑6], as evidenced by ELISA. Furthermore, caerulein‑induced apoptosis was reported by flow cytometry and western blot assays. Additionally, miR‑9 expression was downregulated by caerulein treatment, as demonstrated by reverse transcription quantitative PCR. However, miR‑9 overexpression reduced the inflammatory response and apoptosis in caerulein‑treated AR42J cells. miR‑9 knockdown resulted in opposite effects. Furthermore, fibroblast growth factor (FGF) 10 was validated to be targeted via miR‑9 by luciferase, RNA immunoprecipitation and RNA pull‑down assays. Results demonstrated increased FGF10 expression in caerulein‑treated AR42J cells and that FGF10 over­expression exacerbated the caerulein‑induced inflammatory response and apoptosis, while its knockdown had the opposite effect. Additionally, FGF10 reversed the effect of miR‑9 on caerulein‑induced injury in AR42J cells. Results demonstrated that miR‑9 inhibited the expression of the nuclear factor κB (NF‑κB) pathway‑related proteins by downregulating FGF10. As a result, miR‑9 decreased inflammatory response and apoptosis in caerulein‑treated AR42J cells by targeting FGF10 and blocking NF‑κB signaling, suggesting that miR‑9 may serve as a novel target for AP treatment.
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Copy and paste a formatted citation
Spandidos Publications style
Shen Y, Xue C, You G and Liu C: miR‑9 alleviated the inflammatory response and apoptosis in caerulein‑induced acute pancreatitis by regulating FGF10 and the NF‑κB signaling pathway. Exp Ther Med 22: 795, 2021.
APA
Shen, Y., Xue, C., You, G., & Liu, C. (2021). miR‑9 alleviated the inflammatory response and apoptosis in caerulein‑induced acute pancreatitis by regulating FGF10 and the NF‑κB signaling pathway. Experimental and Therapeutic Medicine, 22, 795. https://doi.org/10.3892/etm.2021.10227
MLA
Shen, Y., Xue, C., You, G., Liu, C."miR‑9 alleviated the inflammatory response and apoptosis in caerulein‑induced acute pancreatitis by regulating FGF10 and the NF‑κB signaling pathway". Experimental and Therapeutic Medicine 22.2 (2021): 795.
Chicago
Shen, Y., Xue, C., You, G., Liu, C."miR‑9 alleviated the inflammatory response and apoptosis in caerulein‑induced acute pancreatitis by regulating FGF10 and the NF‑κB signaling pathway". Experimental and Therapeutic Medicine 22, no. 2 (2021): 795. https://doi.org/10.3892/etm.2021.10227
Copy and paste a formatted citation
x
Spandidos Publications style
Shen Y, Xue C, You G and Liu C: miR‑9 alleviated the inflammatory response and apoptosis in caerulein‑induced acute pancreatitis by regulating FGF10 and the NF‑κB signaling pathway. Exp Ther Med 22: 795, 2021.
APA
Shen, Y., Xue, C., You, G., & Liu, C. (2021). miR‑9 alleviated the inflammatory response and apoptosis in caerulein‑induced acute pancreatitis by regulating FGF10 and the NF‑κB signaling pathway. Experimental and Therapeutic Medicine, 22, 795. https://doi.org/10.3892/etm.2021.10227
MLA
Shen, Y., Xue, C., You, G., Liu, C."miR‑9 alleviated the inflammatory response and apoptosis in caerulein‑induced acute pancreatitis by regulating FGF10 and the NF‑κB signaling pathway". Experimental and Therapeutic Medicine 22.2 (2021): 795.
Chicago
Shen, Y., Xue, C., You, G., Liu, C."miR‑9 alleviated the inflammatory response and apoptosis in caerulein‑induced acute pancreatitis by regulating FGF10 and the NF‑κB signaling pathway". Experimental and Therapeutic Medicine 22, no. 2 (2021): 795. https://doi.org/10.3892/etm.2021.10227
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