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MicroRNA‑145‑5p inhibits hypoxia/reoxygenation‑induced apoptosis in H9c2 cardiomyocytes by targeting ROCK1

  • Authors:
    • Chao Cheng
    • Dong-Ling Xu
    • Xiao-Bo Liu
    • Shao-Jie Bi
    • Juan Zhang
  • View Affiliations / Copyright

    Affiliations: Department of Cardiology, The Second Hospital, Cheeloo College of Medicine, Shandong University, Jinan, Shandong 250033, P.R. China, Shandong Blood Center, Jinan, Shandong 250012, P.R. China
    Copyright: © Cheng et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 796
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    Published online on: May 25, 2021
       https://doi.org/10.3892/etm.2021.10228
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Abstract

There is increasing evidence that microRNAs (miRs) play critical roles in the pathological and physiological processes associated with myocardial ischemia reperfusion (I/R). miR‑145 has been extensively studied in the cardiovascular system; however, the role of miR‑145 in myocardial I/R remains unclear. Therefore, the present study aimed to investigate the role and mechanism of miR‑145‑5p in myocardial I/R by establishing a hypoxia/reoxygenation (H/R) model using H9c2 cardiomyocytes. The expression of miR‑145‑5p was regulated by transfection and the potential target of miR‑145‑5p was identified. In addition, apoptosis of the cardiomyocytes was evaluated using flow cytometry and the detection of cleaved caspase‑3 by western blotting. The results revealed that miR‑145‑5p expression was decreased while cell apoptosis and Rho‑associated coiled‑coil‑containing kinase 1 (ROCK1) expression were increased in H/R‑stimulated H9c2 cardiomyocytes. The upregulation of miR‑145‑5p reduced apoptosis and the expression of ROCK1 in H/R‑stimulated H9c2 cardiomyocytes. Furthermore, the overexpression of ROCK1 significantly attenuated the miR‑145‑5p‑induced reduction of apoptosis following H/R. In conclusion, the present study indicates that the overexpression of miR‑145‑5p inhibits H/R‑induced cardiomyocyte apoptosis by targeting ROCK1.
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Copy and paste a formatted citation
Spandidos Publications style
Cheng C, Xu D, Liu X, Bi S and Zhang J: MicroRNA‑145‑5p inhibits hypoxia/reoxygenation‑induced apoptosis in H9c2 cardiomyocytes by targeting ROCK1. Exp Ther Med 22: 796, 2021.
APA
Cheng, C., Xu, D., Liu, X., Bi, S., & Zhang, J. (2021). MicroRNA‑145‑5p inhibits hypoxia/reoxygenation‑induced apoptosis in H9c2 cardiomyocytes by targeting ROCK1. Experimental and Therapeutic Medicine, 22, 796. https://doi.org/10.3892/etm.2021.10228
MLA
Cheng, C., Xu, D., Liu, X., Bi, S., Zhang, J."MicroRNA‑145‑5p inhibits hypoxia/reoxygenation‑induced apoptosis in H9c2 cardiomyocytes by targeting ROCK1". Experimental and Therapeutic Medicine 22.2 (2021): 796.
Chicago
Cheng, C., Xu, D., Liu, X., Bi, S., Zhang, J."MicroRNA‑145‑5p inhibits hypoxia/reoxygenation‑induced apoptosis in H9c2 cardiomyocytes by targeting ROCK1". Experimental and Therapeutic Medicine 22, no. 2 (2021): 796. https://doi.org/10.3892/etm.2021.10228
Copy and paste a formatted citation
x
Spandidos Publications style
Cheng C, Xu D, Liu X, Bi S and Zhang J: MicroRNA‑145‑5p inhibits hypoxia/reoxygenation‑induced apoptosis in H9c2 cardiomyocytes by targeting ROCK1. Exp Ther Med 22: 796, 2021.
APA
Cheng, C., Xu, D., Liu, X., Bi, S., & Zhang, J. (2021). MicroRNA‑145‑5p inhibits hypoxia/reoxygenation‑induced apoptosis in H9c2 cardiomyocytes by targeting ROCK1. Experimental and Therapeutic Medicine, 22, 796. https://doi.org/10.3892/etm.2021.10228
MLA
Cheng, C., Xu, D., Liu, X., Bi, S., Zhang, J."MicroRNA‑145‑5p inhibits hypoxia/reoxygenation‑induced apoptosis in H9c2 cardiomyocytes by targeting ROCK1". Experimental and Therapeutic Medicine 22.2 (2021): 796.
Chicago
Cheng, C., Xu, D., Liu, X., Bi, S., Zhang, J."MicroRNA‑145‑5p inhibits hypoxia/reoxygenation‑induced apoptosis in H9c2 cardiomyocytes by targeting ROCK1". Experimental and Therapeutic Medicine 22, no. 2 (2021): 796. https://doi.org/10.3892/etm.2021.10228
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