Open Access

MicroRNA‑145‑5p inhibits hypoxia/reoxygenation‑induced apoptosis in H9c2 cardiomyocytes by targeting ROCK1

  • Authors:
    • Chao Cheng
    • Dong-Ling Xu
    • Xiao-Bo Liu
    • Shao-Jie Bi
    • Juan Zhang
  • View Affiliations

  • Published online on: May 25, 2021     https://doi.org/10.3892/etm.2021.10228
  • Article Number: 796
  • Copyright: © Cheng et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

There is increasing evidence that microRNAs (miRs) play critical roles in the pathological and physiological processes associated with myocardial ischemia reperfusion (I/R). miR‑145 has been extensively studied in the cardiovascular system; however, the role of miR‑145 in myocardial I/R remains unclear. Therefore, the present study aimed to investigate the role and mechanism of miR‑145‑5p in myocardial I/R by establishing a hypoxia/reoxygenation (H/R) model using H9c2 cardiomyocytes. The expression of miR‑145‑5p was regulated by transfection and the potential target of miR‑145‑5p was identified. In addition, apoptosis of the cardiomyocytes was evaluated using flow cytometry and the detection of cleaved caspase‑3 by western blotting. The results revealed that miR‑145‑5p expression was decreased while cell apoptosis and Rho‑associated coiled‑coil‑containing kinase 1 (ROCK1) expression were increased in H/R‑stimulated H9c2 cardiomyocytes. The upregulation of miR‑145‑5p reduced apoptosis and the expression of ROCK1 in H/R‑stimulated H9c2 cardiomyocytes. Furthermore, the overexpression of ROCK1 significantly attenuated the miR‑145‑5p‑induced reduction of apoptosis following H/R. In conclusion, the present study indicates that the overexpression of miR‑145‑5p inhibits H/R‑induced cardiomyocyte apoptosis by targeting ROCK1.
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August-2021
Volume 22 Issue 2

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Cheng C, Xu D, Liu X, Bi S and Zhang J: MicroRNA‑145‑5p inhibits hypoxia/reoxygenation‑induced apoptosis in H9c2 cardiomyocytes by targeting ROCK1. Exp Ther Med 22: 796, 2021
APA
Cheng, C., Xu, D., Liu, X., Bi, S., & Zhang, J. (2021). MicroRNA‑145‑5p inhibits hypoxia/reoxygenation‑induced apoptosis in H9c2 cardiomyocytes by targeting ROCK1. Experimental and Therapeutic Medicine, 22, 796. https://doi.org/10.3892/etm.2021.10228
MLA
Cheng, C., Xu, D., Liu, X., Bi, S., Zhang, J."MicroRNA‑145‑5p inhibits hypoxia/reoxygenation‑induced apoptosis in H9c2 cardiomyocytes by targeting ROCK1". Experimental and Therapeutic Medicine 22.2 (2021): 796.
Chicago
Cheng, C., Xu, D., Liu, X., Bi, S., Zhang, J."MicroRNA‑145‑5p inhibits hypoxia/reoxygenation‑induced apoptosis in H9c2 cardiomyocytes by targeting ROCK1". Experimental and Therapeutic Medicine 22, no. 2 (2021): 796. https://doi.org/10.3892/etm.2021.10228