Open Access

Blocking TNFα attenuates progressive cartilage matrix degradation in inflammatory arthritis

  • Authors:
    • Jinsung Park
    • Hyosun Park
    • Young Lim Lee
    • Subin Weon
    • Yong-Gil Kim
    • Jae-Hyuk Yang
    • Bora Nam
    • Sungsin Jo
    • Tae-Hwan Kim
  • View Affiliations

  • Published online on: May 27, 2021     https://doi.org/10.3892/etm.2021.10240
  • Article Number: 808
  • Copyright: © Park et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Because damage to hyaline cartilage is irreversible, relieving progressive cartilage destruction is an important therapeutic approach for inflammatory arthritis. In the present study, human hyaline chondrocytes were isolated from total knee replacements of 15 patients with osteoarthritis (OA) and three with rheumatoid arthritis (RA). Synovial fluid of OA (n=25) and RA (n=34) were collected to measure tumor necrosis factor α (TNFα) using ELISA. Consistent with previous studies, the synovial fluid exhibited high TNFα levels and hyaline cartilage was severely destroyed in patients with RA. TNFα‑treated chondrocytes were used as model for inflammatory arthritis. TNFα did not influence proliferation or extracellular matrix expression in chondrocytes, but induced matrix metalloproteinase (MMP)1, 3 and 13 expression levels in chondrocytes, which was accompanied by activation of nuclear factor‑κB signaling. During chondrogenic differentiation, TNFα attenuated mRNA expression levels of anabolic factors (collagen type 2 and aggrecan) and enhanced mRNA expression of catabolic factors (MMP1, MMP3 and MMP13) in chondrocytes. Moreover, anti‑TNFα agents (Golimumab) inhibited the TNFα‑induced metabolic shift in chondrocytes and chondrogenic differentiation. The present study revealed a mechanism by which TNFα may induce metabolic shift in chondrocytes, leading to progressive chondrocyte destruction.
View Figures
View References

Related Articles

Journal Cover

August-2021
Volume 22 Issue 2

Print ISSN: 1792-0981
Online ISSN:1792-1015

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Park J, Park H, Lee YL, Weon S, Kim Y, Yang J, Nam B, Jo S and Kim T: Blocking TNFα attenuates progressive cartilage matrix degradation in inflammatory arthritis. Exp Ther Med 22: 808, 2021.
APA
Park, J., Park, H., Lee, Y.L., Weon, S., Kim, Y., Yang, J. ... Kim, T. (2021). Blocking TNFα attenuates progressive cartilage matrix degradation in inflammatory arthritis. Experimental and Therapeutic Medicine, 22, 808. https://doi.org/10.3892/etm.2021.10240
MLA
Park, J., Park, H., Lee, Y. L., Weon, S., Kim, Y., Yang, J., Nam, B., Jo, S., Kim, T."Blocking TNFα attenuates progressive cartilage matrix degradation in inflammatory arthritis". Experimental and Therapeutic Medicine 22.2 (2021): 808.
Chicago
Park, J., Park, H., Lee, Y. L., Weon, S., Kim, Y., Yang, J., Nam, B., Jo, S., Kim, T."Blocking TNFα attenuates progressive cartilage matrix degradation in inflammatory arthritis". Experimental and Therapeutic Medicine 22, no. 2 (2021): 808. https://doi.org/10.3892/etm.2021.10240