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miR‑452‑5p regulates the responsiveness of intestinal epithelial cells in inflammatory bowel disease through Mcl‑1

  • Authors:
    • Ming Deng
    • Jianglin Hu
    • Rui Tong
    • Hongming Guo
    • Xuehui Li
    • Yan Liu
  • View Affiliations / Copyright

    Affiliations: Department of Anorectal Surgery, People's Hospital of Dongxihu District, Wuhan, Hubei 430040, P.R. China, Department of Gastrointestinal Surgery, Union Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430022, P.R. China
    Copyright: © Deng et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 813
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    Published online on: May 28, 2021
       https://doi.org/10.3892/etm.2021.10245
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Abstract

Inflammatory bowel diseases (IBDs) are chronic immune disorders that occur in the intestinal tract. Previous studies have revealed that intestinal epithelial cells (IECs) play critical roles in the development of IBDs, and therapies targeting IECs hold great potential for the treatment of IBDs. However, the roles of microRNAs (miRs) in the regulation of IEC properties and whether they can be used as targets for IEC regulation and IBD treatment are largely unknown. The aim of the present study was to investigate the role of the miR‑452‑5p/Mcl‑1 axis in the regulation of the properties of IECs during the pathology of IBD. A dextran sulfate sodium‑induced mouse model of ulcerative colitis (UC) and an in vitro lipopolysaccharide‑stimulated IEC‑6 cell model were investigated. The results revealed that miR‑452‑5p expression in the IECs of the mice increased significantly upon UC induction, and the knockdown of miR‑452‑5p alleviated the IBD symptoms. Furthermore, the suppression of miR‑452‑5p downregulated the expression of the inflammatory cytokines IL‑6, IL‑8 and TNFα, and upregulated the expression of intestinal barrier‑associated molecules, namely occludin, zona occludens 1 and mucin‑2 in IECs in vitro and in vivo. Notably, the results indicated that miR‑452‑5p modulated the responses of IECs by negatively regulating the expression of Mcl‑1, as the knockdown of Mcl‑1 abrogated the effects of miR‑452‑5p suppression on IECs. The present study suggested that miR‑452‑5p regulated the responsiveness of IECs to influence the development of UC in an Mcl‑1‑dependent manner. These observations provide important information to improve the understanding of IBD pathogenesis and indicate that targeting the miR‑452‑5p‑Mcl‑1 signaling axis in IECs holds potential for IBD treatment.
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Copy and paste a formatted citation
Spandidos Publications style
Deng M, Hu J, Tong R, Guo H, Li X and Liu Y: miR‑452‑5p regulates the responsiveness of intestinal epithelial cells in inflammatory bowel disease through Mcl‑1. Exp Ther Med 22: 813, 2021.
APA
Deng, M., Hu, J., Tong, R., Guo, H., Li, X., & Liu, Y. (2021). miR‑452‑5p regulates the responsiveness of intestinal epithelial cells in inflammatory bowel disease through Mcl‑1. Experimental and Therapeutic Medicine, 22, 813. https://doi.org/10.3892/etm.2021.10245
MLA
Deng, M., Hu, J., Tong, R., Guo, H., Li, X., Liu, Y."miR‑452‑5p regulates the responsiveness of intestinal epithelial cells in inflammatory bowel disease through Mcl‑1". Experimental and Therapeutic Medicine 22.2 (2021): 813.
Chicago
Deng, M., Hu, J., Tong, R., Guo, H., Li, X., Liu, Y."miR‑452‑5p regulates the responsiveness of intestinal epithelial cells in inflammatory bowel disease through Mcl‑1". Experimental and Therapeutic Medicine 22, no. 2 (2021): 813. https://doi.org/10.3892/etm.2021.10245
Copy and paste a formatted citation
x
Spandidos Publications style
Deng M, Hu J, Tong R, Guo H, Li X and Liu Y: miR‑452‑5p regulates the responsiveness of intestinal epithelial cells in inflammatory bowel disease through Mcl‑1. Exp Ther Med 22: 813, 2021.
APA
Deng, M., Hu, J., Tong, R., Guo, H., Li, X., & Liu, Y. (2021). miR‑452‑5p regulates the responsiveness of intestinal epithelial cells in inflammatory bowel disease through Mcl‑1. Experimental and Therapeutic Medicine, 22, 813. https://doi.org/10.3892/etm.2021.10245
MLA
Deng, M., Hu, J., Tong, R., Guo, H., Li, X., Liu, Y."miR‑452‑5p regulates the responsiveness of intestinal epithelial cells in inflammatory bowel disease through Mcl‑1". Experimental and Therapeutic Medicine 22.2 (2021): 813.
Chicago
Deng, M., Hu, J., Tong, R., Guo, H., Li, X., Liu, Y."miR‑452‑5p regulates the responsiveness of intestinal epithelial cells in inflammatory bowel disease through Mcl‑1". Experimental and Therapeutic Medicine 22, no. 2 (2021): 813. https://doi.org/10.3892/etm.2021.10245
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