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Effect of the ACY‑1 gene on HER2 and TRAIL expression in rectal carcinoma

  • Authors:
    • Zizhong Xu
    • Yating Hu
    • Zhaohui Yu
  • View Affiliations / Copyright

    Affiliations: Department of General Surgery, The First People's Hospital Xianyang City, Xianyang, Shanxi 712000, P.R. China, Department of Endocrinology, The First People's Hospital Xianyang City, Xianyang, Shanxi 712000, P.R. China, Department of Anorectal, The First People's Hospital Xianyang City, Xianyang, Shanxi 712000, P.R. China
    Copyright: © Xu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 817
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    Published online on: June 2, 2021
       https://doi.org/10.3892/etm.2021.10249
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Abstract

The incidence of rectal carcinoma (RC) is increasing and the age at onset of the disease is reducing. Therefore, elucidating the pathogenesis of RC is beneficial for early diagnosis and improving the prognosis. Aminoacylase‑1 (ACY‑1) is abnormally expressed in various malignant tumor tissues. Furthermore, the human epidermal growth factor receptor‑2 (HER2) gene is involved in tumor metastasis and invasion, while tumor necrosis factor‑related apoptosis‑inducing ligand (TRAIL) induces tumor cell apoptosis. The aim of the present study was to investigate the effect of the ACY‑1 gene on the expression of HER2 and TRAIL in RC. Cancerous and adjacent tissues from RC patients were collected. ACY‑1 expression was analyzed by immunohistochemistry. The rectal cancer cell lines HT29 and SW620, and normal colorectal mucosal epithelial fetal human cells were cultured in vitro. ACY‑1 gene and protein expression levels were tested by reverse transcription‑quantitative PCR and western blotting. ACY‑1 small interfering RNA (siRNA) was transfected into HT29 and SW620 cells. Cell proliferation was detected by thiazolyl blue MTT assay. Caspase‑3 activity was assessed using a commercial kit. HER2 and TRAIL expression levels were determined by western blotting. ACY‑1 expression was significantly increased in cancer tissue compared with adjacent tissue (P<0.05). ACY‑1 expression was elevated in HT29 and SW620 cells compared with normal colorectal mucosal epithelial cells (P<0.05). ACY‑1 siRNA transfected into HT29 cells downregulated its expression, inhibited cell proliferation, enhanced caspase‑3 activity, reduced HER2 expression and upregulated TRAIL expression (P<0.05). ACY‑1 expression was found to be increased in rectal cancer tissue. Therefore, targeting the ACY‑1 gene may regulate HER2 and TRAIL expression levels, and may reduce the occurrence and inhibit the development of rectal cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Xu Z, Hu Y and Yu Z: Effect of the ACY‑1 gene on HER2 and TRAIL expression in rectal carcinoma. Exp Ther Med 22: 817, 2021.
APA
Xu, Z., Hu, Y., & Yu, Z. (2021). Effect of the ACY‑1 gene on HER2 and TRAIL expression in rectal carcinoma. Experimental and Therapeutic Medicine, 22, 817. https://doi.org/10.3892/etm.2021.10249
MLA
Xu, Z., Hu, Y., Yu, Z."Effect of the ACY‑1 gene on HER2 and TRAIL expression in rectal carcinoma". Experimental and Therapeutic Medicine 22.2 (2021): 817.
Chicago
Xu, Z., Hu, Y., Yu, Z."Effect of the ACY‑1 gene on HER2 and TRAIL expression in rectal carcinoma". Experimental and Therapeutic Medicine 22, no. 2 (2021): 817. https://doi.org/10.3892/etm.2021.10249
Copy and paste a formatted citation
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Spandidos Publications style
Xu Z, Hu Y and Yu Z: Effect of the ACY‑1 gene on HER2 and TRAIL expression in rectal carcinoma. Exp Ther Med 22: 817, 2021.
APA
Xu, Z., Hu, Y., & Yu, Z. (2021). Effect of the ACY‑1 gene on HER2 and TRAIL expression in rectal carcinoma. Experimental and Therapeutic Medicine, 22, 817. https://doi.org/10.3892/etm.2021.10249
MLA
Xu, Z., Hu, Y., Yu, Z."Effect of the ACY‑1 gene on HER2 and TRAIL expression in rectal carcinoma". Experimental and Therapeutic Medicine 22.2 (2021): 817.
Chicago
Xu, Z., Hu, Y., Yu, Z."Effect of the ACY‑1 gene on HER2 and TRAIL expression in rectal carcinoma". Experimental and Therapeutic Medicine 22, no. 2 (2021): 817. https://doi.org/10.3892/etm.2021.10249
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