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miR‑133a‑3p regulates the proliferation and apoptosis of intestinal epithelial cells by modulating the expression of TAGLN2

  • Authors:
    • Xiaoxi Tian
    • Lihong Li
    • Guoqiang Fu
    • Jianyu Wang
    • Qianfeng  He
    • Cuicui Zhang
    • Bingrui Qin
    • Jiahui Wang
  • View Affiliations / Copyright

    Affiliations: Department of Emergency, Tangdu Hospital of Air Force Military Medical University, Xi'an, Shaanxi 710038, P.R. China, Medical College of Air Force Military Medical University, Xi'an, Shaanxi 710032, P.R. China
    Copyright: © Tian et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 824
    |
    Published online on: June 2, 2021
       https://doi.org/10.3892/etm.2021.10256
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Abstract

Sepsis is one of the most common diseases in patients in intensive care units. Intestinal barrier dysfunction serves a critical role in the pathogenesis and progression of sepsis. Therefore, preservation of the intestinal epithelial barrier function is an area of ongoing research in the treatment of sepsis. The present study investigated the effects of miR‑133a‑3p on the proliferation and apoptosis of intestinal epithelial cells and the possible mechanism underlying its actions. miR‑133a‑3p was used to upregulate the intestinal epithelial FHs 74 Int cell line and cell proliferation and apoptosis were investigated. A luciferase reporter assay was used to determine whether the 3'‑UTR of TAGLN2 mRNA was a binding target of miR‑133a‑3p. FHs 74 Int cells were transfected with TAGLN2 shRNA and the effects of TAGLN2 on the proliferation and apoptosis of intestinal epithelial cells were investigated. It was found that miR‑133a‑3p inhibited the proliferation and promoted the apoptosis of intestinal epithelial cells. A luciferase reporter assay confirmed that miR‑133a‑3p targeted TAGLN2 directly. In addition, low expression of TAGLN2 inhibited the proliferation and promoted the apoptosis of intestinal epithelial cells. The results of the present study suggested that the miR‑133a‑3p inhibition of proliferation and promotion of apoptosis occurred via the inhibition of TAGLN2. These results suggested that miR‑133a‑3p may be a promising therapeutic target for the diagnosis and treatment of gut‑origin sepsis.
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Copy and paste a formatted citation
Spandidos Publications style
Tian X, Li L, Fu G, Wang J, He Q, Zhang C, Qin B and Wang J: miR‑133a‑3p regulates the proliferation and apoptosis of intestinal epithelial cells by modulating the expression of TAGLN2. Exp Ther Med 22: 824, 2021.
APA
Tian, X., Li, L., Fu, G., Wang, J., He, Q., Zhang, C. ... Wang, J. (2021). miR‑133a‑3p regulates the proliferation and apoptosis of intestinal epithelial cells by modulating the expression of TAGLN2. Experimental and Therapeutic Medicine, 22, 824. https://doi.org/10.3892/etm.2021.10256
MLA
Tian, X., Li, L., Fu, G., Wang, J., He, Q., Zhang, C., Qin, B., Wang, J."miR‑133a‑3p regulates the proliferation and apoptosis of intestinal epithelial cells by modulating the expression of TAGLN2". Experimental and Therapeutic Medicine 22.2 (2021): 824.
Chicago
Tian, X., Li, L., Fu, G., Wang, J., He, Q., Zhang, C., Qin, B., Wang, J."miR‑133a‑3p regulates the proliferation and apoptosis of intestinal epithelial cells by modulating the expression of TAGLN2". Experimental and Therapeutic Medicine 22, no. 2 (2021): 824. https://doi.org/10.3892/etm.2021.10256
Copy and paste a formatted citation
x
Spandidos Publications style
Tian X, Li L, Fu G, Wang J, He Q, Zhang C, Qin B and Wang J: miR‑133a‑3p regulates the proliferation and apoptosis of intestinal epithelial cells by modulating the expression of TAGLN2. Exp Ther Med 22: 824, 2021.
APA
Tian, X., Li, L., Fu, G., Wang, J., He, Q., Zhang, C. ... Wang, J. (2021). miR‑133a‑3p regulates the proliferation and apoptosis of intestinal epithelial cells by modulating the expression of TAGLN2. Experimental and Therapeutic Medicine, 22, 824. https://doi.org/10.3892/etm.2021.10256
MLA
Tian, X., Li, L., Fu, G., Wang, J., He, Q., Zhang, C., Qin, B., Wang, J."miR‑133a‑3p regulates the proliferation and apoptosis of intestinal epithelial cells by modulating the expression of TAGLN2". Experimental and Therapeutic Medicine 22.2 (2021): 824.
Chicago
Tian, X., Li, L., Fu, G., Wang, J., He, Q., Zhang, C., Qin, B., Wang, J."miR‑133a‑3p regulates the proliferation and apoptosis of intestinal epithelial cells by modulating the expression of TAGLN2". Experimental and Therapeutic Medicine 22, no. 2 (2021): 824. https://doi.org/10.3892/etm.2021.10256
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