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MEF/KSF‑conditioned culture medium: An effective method for in vitro culture of mouse dermal papilla cells with osteogenic differentiation potential

  • Authors:
    • Liang Xu
    • Wenlan Gao
    • Shanshan Bai
    • Huichuan Duan
    • Xiaogang Pan
    • Wei Wu
  • View Affiliations / Copyright

    Affiliations: Department of Plastic and Reconstructive Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, P.R. China, Department of Stomatology, Shanghai Children's Medical Center, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, P.R. China, Department of Orthodontics, Shanghai Ninth Peoples' Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, P.R. China
    Copyright: © Xu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 828
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    Published online on: June 3, 2021
       https://doi.org/10.3892/etm.2021.10260
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Abstract

Hair follicle stem cells are pluripotent and have a self‑renewal capacity and multi‑differentiation potential in vitro. As hair follicle stem cells can be easily sampled from the skin and hair of clinical patients at a considerable quantity, these cells have potential applications in wound repair and skin tissue engineering. Effective approaches for the in vitro culture and amplification of mouse hair follicle stem cells, as well as the in vitro osteogenic differentiation potential and cell source when obtaining mouse‑separated cells were examined. Serial subculture was performed in different culture systems. Cell source was detected based on the relevant surface markers derived from mouse hair follicles at the gene and protein levels, and the differential potential was determined. The proliferative ability of hair follicle‑derived stem cells obtained from mouse embryonic fibroblast (MEF)/keratinocyte serum‑free medium (KSF)‑conditioned medium was the highest among all culture systems. The induced group had a stronger osteogenic differentiation potential compared with the non‑induced group, indicating that the cells obtained from MEF/KSF‑conditioned medium were cells derived from the hair follicle dermal papilla. Therefore, the strong osteogenic differentiation potential of the hair follicle‑derived mesenchymal stem cells was screened with MEF/KSF‑conditioned culture medium following amplification, and biological characteristics similar to those of hair follicle dermal papilla cells were observed.
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Copy and paste a formatted citation
Spandidos Publications style
Xu L, Gao W, Bai S, Duan H, Pan X and Wu W: MEF/KSF‑conditioned culture medium: An effective method for <em>in vitro</em> culture of mouse dermal papilla cells with osteogenic differentiation potential. Exp Ther Med 22: 828, 2021.
APA
Xu, L., Gao, W., Bai, S., Duan, H., Pan, X., & Wu, W. (2021). MEF/KSF‑conditioned culture medium: An effective method for <em>in vitro</em> culture of mouse dermal papilla cells with osteogenic differentiation potential. Experimental and Therapeutic Medicine, 22, 828. https://doi.org/10.3892/etm.2021.10260
MLA
Xu, L., Gao, W., Bai, S., Duan, H., Pan, X., Wu, W."MEF/KSF‑conditioned culture medium: An effective method for <em>in vitro</em> culture of mouse dermal papilla cells with osteogenic differentiation potential". Experimental and Therapeutic Medicine 22.2 (2021): 828.
Chicago
Xu, L., Gao, W., Bai, S., Duan, H., Pan, X., Wu, W."MEF/KSF‑conditioned culture medium: An effective method for <em>in vitro</em> culture of mouse dermal papilla cells with osteogenic differentiation potential". Experimental and Therapeutic Medicine 22, no. 2 (2021): 828. https://doi.org/10.3892/etm.2021.10260
Copy and paste a formatted citation
x
Spandidos Publications style
Xu L, Gao W, Bai S, Duan H, Pan X and Wu W: MEF/KSF‑conditioned culture medium: An effective method for <em>in vitro</em> culture of mouse dermal papilla cells with osteogenic differentiation potential. Exp Ther Med 22: 828, 2021.
APA
Xu, L., Gao, W., Bai, S., Duan, H., Pan, X., & Wu, W. (2021). MEF/KSF‑conditioned culture medium: An effective method for <em>in vitro</em> culture of mouse dermal papilla cells with osteogenic differentiation potential. Experimental and Therapeutic Medicine, 22, 828. https://doi.org/10.3892/etm.2021.10260
MLA
Xu, L., Gao, W., Bai, S., Duan, H., Pan, X., Wu, W."MEF/KSF‑conditioned culture medium: An effective method for <em>in vitro</em> culture of mouse dermal papilla cells with osteogenic differentiation potential". Experimental and Therapeutic Medicine 22.2 (2021): 828.
Chicago
Xu, L., Gao, W., Bai, S., Duan, H., Pan, X., Wu, W."MEF/KSF‑conditioned culture medium: An effective method for <em>in vitro</em> culture of mouse dermal papilla cells with osteogenic differentiation potential". Experimental and Therapeutic Medicine 22, no. 2 (2021): 828. https://doi.org/10.3892/etm.2021.10260
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