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Anti‑inflammatory activity of different isolated sites of Chloranthus serratus in complete Freund's adjuvant‑induced arthritic rats

  • Authors:
    • Shuping Sun
    • Yunyan Du
    • Shengli Li
    • Bianbian Gao
    • Rongping Xia
    • Wenjing Cao
    • Chao Zhang
    • Enze Zhu
  • View Affiliations / Copyright

    Affiliations: Pharmacy Teaching and Research Department, College of Pharmacy, Wannan Medical College, Wuhu, Anhui 241002, P.R. China, The Third Orthopedics Department, The Fifth People's Hospital of Wuhu, Wuhu, Anhui 241000, P.R. China
    Copyright: © Sun et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 848
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    Published online on: June 8, 2021
       https://doi.org/10.3892/etm.2021.10280
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Abstract

Chloranthus serratus is a traditional Chinese medicine for treating arthritis and bruises. The aim of the present study was to investigate the anti‑arthritic activities and possible associated mechanisms of different isolated sites of Chloranthus serratus (DISC) in adjuvant‑induced arthritis (AA) rats. The therapeutic effects of the extracts were assessed through changes in body weights, swelling rates, arthritis indexes (AI) and organ indexes. The levels of nitric oxide (NO), malondialdehyde and superoxide dismutase were determined using one‑step method, TBA method and hydroxylamine method, respectively; the levels of TNF‑α, IL‑1β, IL‑6, prostaglandin E2, macrophage inhibitor factor‑1, VEGF, immunoglobulin (Ig) G, IgM and IFN‑γ in serum were determined using ELISA. Pathological changes and positive expression of VEGF in the ankle joints were investigated using hematoxylin‑eosin staining and immunohistochemical staining, respectively. DISC treatment increased the weight gains and thymus indexes, and decreased the swelling rates, spleen indexes and AI in AA rats. The water isolated site (WA) and ethyl acetate isolated site (EA) significantly reversed complete Freund's adjuvant (CFA)‑induced changes in the levels of NO, IL‑6, TNF‑α, IgG and IFN‑γ, while the n‑butanol isolated site (NB) only reversed the changes in IL‑6 and IgG contents. Some changes in the chloroform isolated site group showed the same trend as those in the model group. The extracts relieved synovial hyperplasia, inflammatory cell infiltration and articular surface defects, and reduced the positive expression rate of VEGF in the synovial tissues of the AA rats to varying degrees. The WA exhibited the most marked effects, followed by the EA and NB, indicating that WA had optimal therapeutic effects on CFA‑induced arthritic rats, which may be mediated by the oxidative stress and inhibition of inflammatory factors. C. serratus may serve as a potential candidate for the treatment of rheumatoid arthritis.
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Copy and paste a formatted citation
Spandidos Publications style
Sun S, Du Y, Li S, Gao B, Xia R, Cao W, Zhang C and Zhu E: Anti‑inflammatory activity of different isolated sites of <em>Chloranthus serratus</em> in complete Freund's adjuvant‑induced arthritic rats. Exp Ther Med 22: 848, 2021.
APA
Sun, S., Du, Y., Li, S., Gao, B., Xia, R., Cao, W. ... Zhu, E. (2021). Anti‑inflammatory activity of different isolated sites of <em>Chloranthus serratus</em> in complete Freund's adjuvant‑induced arthritic rats. Experimental and Therapeutic Medicine, 22, 848. https://doi.org/10.3892/etm.2021.10280
MLA
Sun, S., Du, Y., Li, S., Gao, B., Xia, R., Cao, W., Zhang, C., Zhu, E."Anti‑inflammatory activity of different isolated sites of <em>Chloranthus serratus</em> in complete Freund's adjuvant‑induced arthritic rats". Experimental and Therapeutic Medicine 22.2 (2021): 848.
Chicago
Sun, S., Du, Y., Li, S., Gao, B., Xia, R., Cao, W., Zhang, C., Zhu, E."Anti‑inflammatory activity of different isolated sites of <em>Chloranthus serratus</em> in complete Freund's adjuvant‑induced arthritic rats". Experimental and Therapeutic Medicine 22, no. 2 (2021): 848. https://doi.org/10.3892/etm.2021.10280
Copy and paste a formatted citation
x
Spandidos Publications style
Sun S, Du Y, Li S, Gao B, Xia R, Cao W, Zhang C and Zhu E: Anti‑inflammatory activity of different isolated sites of <em>Chloranthus serratus</em> in complete Freund's adjuvant‑induced arthritic rats. Exp Ther Med 22: 848, 2021.
APA
Sun, S., Du, Y., Li, S., Gao, B., Xia, R., Cao, W. ... Zhu, E. (2021). Anti‑inflammatory activity of different isolated sites of <em>Chloranthus serratus</em> in complete Freund's adjuvant‑induced arthritic rats. Experimental and Therapeutic Medicine, 22, 848. https://doi.org/10.3892/etm.2021.10280
MLA
Sun, S., Du, Y., Li, S., Gao, B., Xia, R., Cao, W., Zhang, C., Zhu, E."Anti‑inflammatory activity of different isolated sites of <em>Chloranthus serratus</em> in complete Freund's adjuvant‑induced arthritic rats". Experimental and Therapeutic Medicine 22.2 (2021): 848.
Chicago
Sun, S., Du, Y., Li, S., Gao, B., Xia, R., Cao, W., Zhang, C., Zhu, E."Anti‑inflammatory activity of different isolated sites of <em>Chloranthus serratus</em> in complete Freund's adjuvant‑induced arthritic rats". Experimental and Therapeutic Medicine 22, no. 2 (2021): 848. https://doi.org/10.3892/etm.2021.10280
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