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Hydroxysafflor Yellow A inhibits the viability and migration of vascular smooth muscle cells induced by serum from rats with chronic renal failure via inactivation of the PI3K/Akt signaling pathway

  • Authors:
    • Changliang Huo
    • Li Wang
    • Qiuli Wang
    • Yanbo Yang
    • Bo Chen
  • View Affiliations / Copyright

    Affiliations: Department of Nephrology, Lianyungang Hospital of Traditional Chinese Medicine, Lianyungang, Jiangsu 222000, P.R. China
    Copyright: © Huo et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 850
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    Published online on: June 8, 2021
       https://doi.org/10.3892/etm.2021.10282
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Abstract

It has been reported that the viability and migration of vascular smooth muscle cells contributes to arteriovenous fistula stenosis. Hydroxysafflor Yellow A (HSYA) has been demonstrated to inhibit the viability and migration of VSMCs by regulating Akt signaling. The present study aimed to investigate the role of HSYA on the viability and migration of human umbilical vein smooth muscle cells (HUVSMCs) following stimulation using serum from rats with chronic renal failure (CRF), and to determine the effects of HSYA on PI3K/Akt signaling. Wistar rats were randomly divided into two groups, control and CRF groups. Serum from each group was collected to stimulate the HUVSMCs. Cell Counting Kit‑8 and wound healing assays were performed to assess cell viability and migration, respectively. Flow cytometry analysis was performed to assess apoptosis, and western blot analysis was performed to detect protein expression levels of PI3K and Akt. Nitric oxide (NO) production was measured using the Nitrate/Nitrite assay kit. The results demonstrated that serum from CRF rats significantly enhanced cell viability, migration and apoptosis, the effects of which were reversed following treatment with HSYA. In addition, CRF serum decreased NO and endothelial NO synthase expression, whilst increasing the protein expression levels of PI3K and phosphorylated‑Akt in HUVSMCs. Notably, treatment with HSYA markedly restored NO production and inactivated the PI3K/Akt signaling pathway. Furthermore, the PI3K/Akt inhibitor, AMG511, exerted similar effects to HSYA. Taken together, the results of the present study suggest that HSYA suppresses cell viability and migration in the presence of CRF serum by inactivating the PI3K/Akt signaling pathway.
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Copy and paste a formatted citation
Spandidos Publications style
Huo C, Wang L, Wang Q, Yang Y and Chen B: Hydroxysafflor Yellow A inhibits the viability and migration of vascular smooth muscle cells induced by serum from rats with chronic renal failure via inactivation of the PI3K/Akt signaling pathway. Exp Ther Med 22: 850, 2021.
APA
Huo, C., Wang, L., Wang, Q., Yang, Y., & Chen, B. (2021). Hydroxysafflor Yellow A inhibits the viability and migration of vascular smooth muscle cells induced by serum from rats with chronic renal failure via inactivation of the PI3K/Akt signaling pathway. Experimental and Therapeutic Medicine, 22, 850. https://doi.org/10.3892/etm.2021.10282
MLA
Huo, C., Wang, L., Wang, Q., Yang, Y., Chen, B."Hydroxysafflor Yellow A inhibits the viability and migration of vascular smooth muscle cells induced by serum from rats with chronic renal failure via inactivation of the PI3K/Akt signaling pathway". Experimental and Therapeutic Medicine 22.2 (2021): 850.
Chicago
Huo, C., Wang, L., Wang, Q., Yang, Y., Chen, B."Hydroxysafflor Yellow A inhibits the viability and migration of vascular smooth muscle cells induced by serum from rats with chronic renal failure via inactivation of the PI3K/Akt signaling pathway". Experimental and Therapeutic Medicine 22, no. 2 (2021): 850. https://doi.org/10.3892/etm.2021.10282
Copy and paste a formatted citation
x
Spandidos Publications style
Huo C, Wang L, Wang Q, Yang Y and Chen B: Hydroxysafflor Yellow A inhibits the viability and migration of vascular smooth muscle cells induced by serum from rats with chronic renal failure via inactivation of the PI3K/Akt signaling pathway. Exp Ther Med 22: 850, 2021.
APA
Huo, C., Wang, L., Wang, Q., Yang, Y., & Chen, B. (2021). Hydroxysafflor Yellow A inhibits the viability and migration of vascular smooth muscle cells induced by serum from rats with chronic renal failure via inactivation of the PI3K/Akt signaling pathway. Experimental and Therapeutic Medicine, 22, 850. https://doi.org/10.3892/etm.2021.10282
MLA
Huo, C., Wang, L., Wang, Q., Yang, Y., Chen, B."Hydroxysafflor Yellow A inhibits the viability and migration of vascular smooth muscle cells induced by serum from rats with chronic renal failure via inactivation of the PI3K/Akt signaling pathway". Experimental and Therapeutic Medicine 22.2 (2021): 850.
Chicago
Huo, C., Wang, L., Wang, Q., Yang, Y., Chen, B."Hydroxysafflor Yellow A inhibits the viability and migration of vascular smooth muscle cells induced by serum from rats with chronic renal failure via inactivation of the PI3K/Akt signaling pathway". Experimental and Therapeutic Medicine 22, no. 2 (2021): 850. https://doi.org/10.3892/etm.2021.10282
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