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Article

STK10 knockout inhibits cell migration and promotes cell proliferation via modulating the activity of ERM and p38 MAPK in prostate cancer cells

  • Authors:
    • Lu Zhang
    • Shun-Yuan Lu
    • Rui Guo
    • Jin-Xia Ma
    • Ling-Yun Tang
    • Jin-Jin Wang
    • Chun-Ling Shen
    • Li-Ming Lu
    • Jie Liu
    • Zhu-Gang Wang
    • Hong-Xin Zhang
  • View Affiliations / Copyright

    Affiliations: Research Center for Experimental Medicine, State Key Laboratory of Medical Genomics, Shanghai Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, P.R. China, Shanghai Model Organisms Center, Shanghai 201321, P.R. China, Shanghai Institute of Immunology, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, P.R. China, Shanghai Institute of Orthopaedics and Traumatology, Shanghai Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, P.R. China
  • Article Number: 851
    |
    Published online on: June 8, 2021
       https://doi.org/10.3892/etm.2021.10283
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Abstract

Prostate cancer (PCa) is one of the most common types of cancer and is a serious threat to men's health due to the high rate of incidence and metastasis. However, the exact underlying pathology of this malignant disease has yet to be fully elucidated. The ezrin‑radixin‑moesin (ERM) family of proteins are associated with the development and metastasis of various types of cancer. Serine threonine kinase 10 (STK10) is an ERM kinase that is involved in the activation of ERM proteins and serves essential roles in the aggregation and adhesion of lymphocytes. To evaluate the functional roles of STK10 in the pathogenesis of PCa, a STK10‑knockout (KO) DU145 PCa cell line was generated using the CRISPR‑Cas9 gene editing system, and the effects of STK10 deletion on tumor biological behaviors were further analyzed. The present data suggested that STK10 KO promoted PCa cell proliferation by inhibiting p38 MAPK activation and suppressed migration primarily via the inhibition of p38 MAPK signaling and ERM protein activation. To the best of our knowledge, this is the first study to provide evidence that STK10 plays important roles in the proliferation and migration of PCa cells, which will be useful for further investigation into the pathogenesis of this disease.
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1 

Bray F, Ferlay J, Soerjomataram I, Siegel RL, Torre LA and Jemal A: Global cancer statistics 2018: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries. CA Cancer J Clin. 68:394–424. 2018.PubMed/NCBI View Article : Google Scholar

2 

Schatten H: Brief overview of prostate cancer statistics, grading, diagnosis and treatment strategies. Adv Exp Med Biol. 1095:1–14. 2018.PubMed/NCBI View Article : Google Scholar

3 

Shah ET, Upadhyaya A, Philp LK, Tang T, Skalamera D, Gunter J, Nelson CC, Williams ED and Hollier BG: Repositioning ‘old’ drugs for new causes: Identifying new inhibitors of prostate cancer cell migration and invasion. Clin Exp Metastasis. 33:385–399. 2016.PubMed/NCBI View Article : Google Scholar

4 

Liu G, Ren F and Song Y: Upregulation of SPOCK2 inhibits the invasion and migration of prostate cancer cells by regulating the MT1-MMP/MMP2 pathway. PeerJ. 7(e7163)2019.PubMed/NCBI View Article : Google Scholar

5 

Tang C, Liu T, Wang K, Wang X, Xu S, He D and Zeng J: Transcriptional regulation of FoxM1 by HIF-1α mediates hypoxia induced EMT in prostate cancer. Oncol Rep. 42:1307–1318. 2019.PubMed/NCBI View Article : Google Scholar

6 

Wang G, Zhao D, Spring DJ and DePinho RA: Genetics and biology of prostate cancer. Genes Dev. 32:1105–1140. 2018.PubMed/NCBI View Article : Google Scholar

7 

Sartor O and de Bono JS: Metastatic prostate cancer. N Engl J Med. 378:645–657. 2018.PubMed/NCBI View Article : Google Scholar

8 

Nguyen DX, Bos PD and Massagué J: Metastasis: From dissemination to organ-specific colonization. Nat Rev Cancer. 9:274–284. 2009.PubMed/NCBI View Article : Google Scholar

9 

Chaffer CL and Weinberg RA: A perspective on cancer cell metastasis. Science. 331:1559–1564. 2011.PubMed/NCBI View Article : Google Scholar

10 

Massagué J and Obenauf AC: Metastatic colonization by circulating tumour cells. Nature. 529:298–306. 2016.PubMed/NCBI View Article : Google Scholar

11 

Arpin M, Chirivino D, Naba A and Zwaenepoel I: Emerging role for ERM proteins in cell adhesion and migration. Cell Adhes Migr. 5:199–206. 2011.PubMed/NCBI View Article : Google Scholar

12 

McClatchey AI: ERM proteins. Curr Biol. 22:R784–R785. 2012.PubMed/NCBI View Article : Google Scholar

13 

Ponuwei GA: A glimpse of the ERM proteins. J Biomed Sci. 23(35)2016.PubMed/NCBI View Article : Google Scholar

14 

Louvet-Vallée S: ERM proteins: From cellular architecture to cell signaling. Biol Cell. 92:305–316. 2000.PubMed/NCBI View Article : Google Scholar

15 

Clucas J and Valderrama F: ERM proteins in cancer progression. J Cell Sci. 128(1253)2015.PubMed/NCBI View Article : Google Scholar

16 

McClatchey AI: ERM proteins at a glance. J Cell Sci. 127:3199–3204. 2014.PubMed/NCBI View Article : Google Scholar

17 

Kuramochi S, Moriguchi T, Kuida K, Endo J, Semba K, Nishida E and Karasuyama H: LOK is a novel mouse STE20-like protein kinase that is expressed predominantly in lymphocytes. J Biol Chem. 272:22679–22684. 1997.PubMed/NCBI View Article : Google Scholar

18 

Belkina NV, Liu Y, Hao JJ, Karasuyama H and Shaw S: LOK is a major ERM kinase in resting lymphocytes and regulates cytoskeletal rearrangement through ERM phosphorylation. Proc Natl Acad Sci USA. 106:4707–4712. 2009.PubMed/NCBI View Article : Google Scholar

19 

Endo J, Toyama-Sorimachi N, Taya C, Kuramochi-Miyagawa S, Nagata K, Kuida K, Takashi T, Yonekawa H, Yoshizawa Y, Miyasaka N, et al: Deficiency of a STE20/PAK family kinase LOK leads to the acceleration of LFA-1 clustering and cell adhesion of activated lymphocytes. FEBS Lett. 468:234–238. 2000.PubMed/NCBI View Article : Google Scholar

20 

Zhang L, Lu SY, Guo R, Ma JX, Tang LY, Shen Y, Shen CL, Lu LM, Wang ZG, Liu J, et al: Knockout of STK10 promotes the migration and invasion of cervical cancer cells. Transl Cancer Res. 9:7079–7090. 2020.

21 

Kupcho K, Shultz J, Hurst R, Hartnett J, Zhou W, Machleidt T, Grailer J, Worzella T, Riss T, Lazar D, et al: A real-time, bioluminescent annexin V assay for the assessment of apoptosis. Apoptosis. 24:184–197. 2019.PubMed/NCBI View Article : Google Scholar

22 

Odero-Marah V, Hawsawi O, Henderson V and Sweeney J: Epithelial-Mesenchymal Transition (EMT) and Prostate Cancer. Adv Exp Med Biol. 1095:101–110. 2018.PubMed/NCBI View Article : Google Scholar

23 

Adjakly M, Ngollo M, Dagdemir A, Judes G, Pajon A, Karsli-Ceppioglu S, Penault-Llorca F, Boiteux JP, Bignon YJ, Guy L, et al: Prostate cancer: The main risk and protective factors-Epigenetic modifications. Ann Endocrinol (Paris). 76:25–41. 2015.PubMed/NCBI View Article : Google Scholar

24 

Gloerich M, Ponsioen B, Vliem MJ, Zhang Z, Zhao J, Kooistra MR, Price LS, Ritsma L, Zwartkruis FJ, Rehmann H, et al: Spatial regulation of cyclic AMP-Epac1 signaling in cell adhesion by ERM proteins. Mol Cell Biol. 30:5421–5431. 2010.PubMed/NCBI View Article : Google Scholar

25 

Lallemand D and Arpin M: Moesin/ezrin: A specific role in cell metastasis? Pigment Cell Melanoma Res. 23:6–7. 2010.PubMed/NCBI View Article : Google Scholar

26 

Koul HK, Pal M and Koul S: Role of p38 MAP kinase signal transduction in solid tumors. Genes Cancer. 4:342–359. 2013.PubMed/NCBI View Article : Google Scholar

27 

Bradham C and McClay DR: p38 MAPK in development and cancer. Cell Cycle. 5:824–828. 2006.PubMed/NCBI View Article : Google Scholar

28 

Moriwaki K and Asahi M: Augmented TME O-GlcNAcylation promotes tumor proliferation through the inhibition of p38 MAPK. Mol Cancer Res. 15:1287–1298. 2017.PubMed/NCBI View Article : Google Scholar

29 

Krebs AM, Mitschke J, Lasierra Losada M, Schmalhofer O, Boerries M, Busch H, Boettcher M, Mougiakakos D, Reichardt W, Bronsert P, et al: The EMT-activator Zeb1 is a key factor for cell plasticity and promotes metastasis in pancreatic cancer. Nat Cell Biol. 19:518–529. 2017.PubMed/NCBI View Article : Google Scholar

30 

Fu M, Wang C, Li Z, Sakamaki T and Pestell RG: Minireview: Cyclin D1: normal and abnormal functions. Endocrinology. 145:5439–5447. 2004.PubMed/NCBI View Article : Google Scholar

31 

Lee RJ, Albanese C, Stenger RJ, Watanabe G, Inghirami G, Haines GK III, Webster M, Muller WJ, Brugge JS, Davis RJ, et al: pp60(v-src) induction of cyclin D1 requires collaborative interactions between the extracellular signal-regulated kinase, p38, and Jun kinase pathways. A role for cAMP response element-binding protein and activating transcription factor-2 in pp60(v-src) signaling in breast cancer cells. J Biol Chem. 274:7341–7350. 1999.PubMed/NCBI View Article : Google Scholar

32 

Wang B, Wang Z, Han L, Gong S, Wang Y, He Z, Feng Y and Yang Z: Prognostic significance of cyclin D3 expression in malignancy patients: A meta-analysis. Cancer Cell Int. 19(158)2019.PubMed/NCBI View Article : Google Scholar

33 

Zhang C, Wu Y, Xuan Z and Zhang S, Wang X, Hao Y, Wu J and Zhang S: p38MAPK, Rho/ROCK and PKC pathways are involved in influenza-induced cytoskeletal rearrangement and hyperpermeability in PMVEC via phosphorylating ERM. Virus Res. 192:6–15. 2014.PubMed/NCBI View Article : Google Scholar

34 

Koss M, Pfeiffer GR II, Wang Y, Thomas ST, Yerukhimovich M, Gaarde WA, Doerschuk CM and Wang Q: Ezrin/radixin/moesin proteins are phosphorylated by TNF-alpha and modulate permeability increases in human pulmonary microvascular endothelial cells. J Immunol. 176:1218–1227. 2006.PubMed/NCBI View Article : Google Scholar

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Copy and paste a formatted citation
Spandidos Publications style
Zhang L, Lu S, Guo R, Ma J, Tang L, Wang J, Shen C, Lu L, Liu J, Wang Z, Wang Z, et al: STK10 knockout inhibits cell migration and promotes cell proliferation via modulating the activity of ERM and p38 MAPK in prostate cancer cells. Exp Ther Med 22: 851, 2021.
APA
Zhang, L., Lu, S., Guo, R., Ma, J., Tang, L., Wang, J. ... Zhang, H. (2021). STK10 knockout inhibits cell migration and promotes cell proliferation via modulating the activity of ERM and p38 MAPK in prostate cancer cells. Experimental and Therapeutic Medicine, 22, 851. https://doi.org/10.3892/etm.2021.10283
MLA
Zhang, L., Lu, S., Guo, R., Ma, J., Tang, L., Wang, J., Shen, C., Lu, L., Liu, J., Wang, Z., Zhang, H."STK10 knockout inhibits cell migration and promotes cell proliferation via modulating the activity of ERM and p38 MAPK in prostate cancer cells". Experimental and Therapeutic Medicine 22.2 (2021): 851.
Chicago
Zhang, L., Lu, S., Guo, R., Ma, J., Tang, L., Wang, J., Shen, C., Lu, L., Liu, J., Wang, Z., Zhang, H."STK10 knockout inhibits cell migration and promotes cell proliferation via modulating the activity of ERM and p38 MAPK in prostate cancer cells". Experimental and Therapeutic Medicine 22, no. 2 (2021): 851. https://doi.org/10.3892/etm.2021.10283
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang L, Lu S, Guo R, Ma J, Tang L, Wang J, Shen C, Lu L, Liu J, Wang Z, Wang Z, et al: STK10 knockout inhibits cell migration and promotes cell proliferation via modulating the activity of ERM and p38 MAPK in prostate cancer cells. Exp Ther Med 22: 851, 2021.
APA
Zhang, L., Lu, S., Guo, R., Ma, J., Tang, L., Wang, J. ... Zhang, H. (2021). STK10 knockout inhibits cell migration and promotes cell proliferation via modulating the activity of ERM and p38 MAPK in prostate cancer cells. Experimental and Therapeutic Medicine, 22, 851. https://doi.org/10.3892/etm.2021.10283
MLA
Zhang, L., Lu, S., Guo, R., Ma, J., Tang, L., Wang, J., Shen, C., Lu, L., Liu, J., Wang, Z., Zhang, H."STK10 knockout inhibits cell migration and promotes cell proliferation via modulating the activity of ERM and p38 MAPK in prostate cancer cells". Experimental and Therapeutic Medicine 22.2 (2021): 851.
Chicago
Zhang, L., Lu, S., Guo, R., Ma, J., Tang, L., Wang, J., Shen, C., Lu, L., Liu, J., Wang, Z., Zhang, H."STK10 knockout inhibits cell migration and promotes cell proliferation via modulating the activity of ERM and p38 MAPK in prostate cancer cells". Experimental and Therapeutic Medicine 22, no. 2 (2021): 851. https://doi.org/10.3892/etm.2021.10283
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