Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Experimental and Therapeutic Medicine
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-0981 Online ISSN: 1792-1015
Journal Cover
August-2021 Volume 22 Issue 2

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
August-2021 Volume 22 Issue 2

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

MicroRNA‑29b participates in the epithelial‑mesenchymal transition of retinal pigment epithelial cells through p‑p65

  • Authors:
    • Min Li
    • Hui Li
    • Shuai Yang
    • Xin Liao
    • Chun Zhao
    • Fang Wang
  • View Affiliations / Copyright

    Affiliations: Department of Ophthalmology, Shanghai Tenth People's Hospital, Shanghai 200072, P.R. China
  • Article Number: 868
    |
    Published online on: June 13, 2021
       https://doi.org/10.3892/etm.2021.10300
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Epithelial‑mesenchymal transition (EMT) of retinal pigment epithelial (RPE) cells is considered to be the main mechanism of proliferative vitreoretinopathy (PVR). Our previous study demonstrated that microRNA‑29b (miR‑29b) and its target protein kinase B (Akt2) played vital roles in this process. miR‑29b, a mesenchymal marker α‑smooth muscle actin (α‑SMA) and the epithelial marker E‑cadherin were assessed in epiretinal membranes of patients with PVR. The potential mechanism of miR‑29b and EMT was also investigated. The expression levels of miR‑29b, E‑cadherin, and α‑SMA in PVR epiretinal membranes were measured using quantitative PCR. The expression levels of Akt2, phosphorylated (p)‑Akt2, p65, p‑p65, and Snail in ARPE‑19 cells were assessed using western blotting. The expression levels of miR‑29b were positively correlated with E‑cadherin mRNA expression, while an inverse correlation was observed between miR‑29b and α‑SMA mRNA expression in epiretinal membranes of patients with PVR. When miR‑29b was transfected into ARPE‑19 cells, the expression levels of Akt2, p‑Akt2, p‑p65 and Snail were downregulated. shRNA‑Akt2 inhibited p‑p65 and Snail expression, while the NF‑κB inhibitor BAY11‑7082 reduced Snail expression. The Akt2/p‑p65/Snail pathway may be the underlying mechanism of miR‑29b in EMT of RPE cells. The results of the present study may provide a new strategy for prevention and therapy of PVR.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

View References

1 

Kwon OW, Song JH and Roh MI: Retinal detachment and proliferative vitreoretinopathy. Dev Ophthalmol. 55:154–162. 2016.PubMed/NCBI View Article : Google Scholar

2 

Pastor JC, de la Rua ER and Martin F: Proliferative vitreoretinopathy: Risk factors and pathobiology. Prog Retin Eye Res. 21:127–144. 2002.PubMed/NCBI View Article : Google Scholar

3 

Saika S, Yamanaka O, Flanders KC, Okada Y, Miyamoto T, Shirai K, Kitano A, Miyazaki K, Tanaka S and Ikeda K: Epithelial-mesenchymal transition as a therapeutic target for prevention of ocular tissue fibrosis. Endocr Metab Immune Disord Drug Targets. 8:69–76. 2008.PubMed/NCBI View Article : Google Scholar

4 

Hoerster R, Muether PS, Vierkotten S, Hermann MM, Kirchhof B and Fauser S: Upregulation of TGF-ß1 in experimental proliferative vitreoretinopathy is accompanied by epithelial to mesenchymal transition. Graefes Arch Clin Exp Ophthalmol. 252:11–16. 2014.PubMed/NCBI View Article : Google Scholar

5 

Li H, Wang HW, Wang F, Gu Q and Xu X: Snail involves in the transforming growth factor β1-mediated epithelial-mesenchymal transition of retinal pigment epithelial cells. PLoS One. 6(e23322)2011.PubMed/NCBI View Article : Google Scholar

6 

Kevin C and Nikolaus R: The evolution of gene regulation by transcription factors and microRNAs. Nat Rev Genet. 8:93–103. 2007.PubMed/NCBI View Article : Google Scholar

7 

Inui M, Martello G and Piccolo S: MicroRNA control of signal transduction. Nat Rev Mol Cell Biol. 11:252–263. 2010.PubMed/NCBI View Article : Google Scholar

8 

Bhatt K, Mi QS and Dong Z: microRNAs in kidneys: Biogenesis, regulation, and pathophysiological roles. Am J Physiol Renal Physiol. 300:F602–F610. 2011.PubMed/NCBI View Article : Google Scholar

9 

Kaneko H and Terasaki H: Biological involvement of microRNAs in proliferative vitreoretinopathy. Transl Vis Sci Technol. 6(5)2017.PubMed/NCBI View Article : Google Scholar

10 

Li M, Li H, Liu XQ, Xu D and Wang F: MicroRNA-29b regulates TGF-β1-mediated epithelial-mesenchymal transition of retinal pigment epithelial cells by targeting AKT2. Exp Cell Res. 345:115–124. 2016.PubMed/NCBI View Article : Google Scholar

11 

Irie HY, Pearline RV, Grueneberg D, Hsia M, Ravichandran P, Kothari N, Natesan S and Brugge JS: Distinct roles of Akt1 and Akt2 in regulating cell migration and epithelial-mesenchymal transition. J Cell Biol. 171:1023–1034. 2005.PubMed/NCBI View Article : Google Scholar

12 

Hotamisligil GS: Endoplasmic reticulum stress and the inflammatory basis of metabolic disease. Cell. 140:900–917. 2010.PubMed/NCBI View Article : Google Scholar

13 

Meng F, Liu L, Chin PC and D'Mello SR: Akt is a downstream target of NF-kappa. B. J Biol Chem. 277:29674–29680. 2002.PubMed/NCBI View Article : Google Scholar

14 

Hong K, Kim JH, Hong JS, Yoon HJ, Lee JI, Hong SP and Hong SD: Inhibition of Akt activity induces the mesenchymal-to-epithelial reverting transition with restoring E-cadherin expression in KB and KOSCC-25B oral squamous cell carcinoma cells. J Exp Clin Cancer Res. 28(28)2009.PubMed/NCBI View Article : Google Scholar

15 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta DeltaC(T)) method. Methods. 25:402–408. 2011.PubMed/NCBI View Article : Google Scholar

16 

World Medical Association (WMA): Declaration of Helsinki. Medical Research Involving Human Subjects. WMA, Ferney-Voltaire 2013. https://www.wma.net/what-we-do/medical-ethics/declaration-of-helsinki/.

17 

Dang H, Ding W, Emerson D and Rountree CB: Snail induces epithelial-to-mesenchymal transition and tumor initiating stem cell characteristics. BMC Cancer. 11(396)2011.PubMed/NCBI View Article : Google Scholar

18 

Cheng ZX, Wang DW, Liu T, Liu WX, Xia WB, Xu J, Zhang YH, Qu YK, Guo LQ, Ding L, et al: Effects of the HIF-1α and NF-κB loop on epithelial-mesenchymal transition and chemoresistance induced by hypoxia in pancreatic cancer cells. Oncol Rep. 31:1891–1898. 2014.PubMed/NCBI View Article : Google Scholar

19 

Cichon MA and Radisky DC: ROS-induced epithelial-mesenchymal transition in mammary epithelial cells is mediated by NF-kB-dependent activation of snail. Oncotarget. 5:2827–2838. 2014.PubMed/NCBI View Article : Google Scholar

20 

Ma J, Zhang L, Hao J, Li N, Tang J and Hao L: Up-regulation of microRNA-93 inhibits TGF-β1-induced EMT and renal fibrogenesis by down-regulation of Orai1. J Pharmacol Sci. 136:218–227. 2018.PubMed/NCBI View Article : Google Scholar

21 

Ma J, Tian K, Wang L, Wang K, Du J, Li D, Wu Z and Zhang J: High expression of TGF-β1 predicting tumor progression in skull base chordomas. World Neurosurg. 131:e265–e270. 2019.PubMed/NCBI View Article : Google Scholar

22 

Cushing L, Kuang PP, Qian J, Shao F, Wu J, Little F, Thannickal VJ, Cardoso WV and Lü J: miR-29 is a major regulator of genes associated with pulmonary fibrosis. Am J Respir Cell Mol Biol. 145:287–294. 2011.PubMed/NCBI View Article : Google Scholar

23 

Arboleda MJ, Lyons JF, Kabbinavar FF, Bray MR, Snow BE, Ayala R, Danino M, Karlan BY and Slamon DJ: Overexpression of AKT2/protein kinase B beta leads to up-regulation of β1 integrins, increased invasion, and metastasis of human breast and ovarian cancer cells. Cancer Res. 63:196–206. 2003.PubMed/NCBI

24 

Yang HW, Kim HJ, Park JH, Shin JM and Lee HM: Apigenin alleviates TGF-β1-induced nasal mucosa remodeling by inhibiting MAPK / NF-kB signaling pathways in chronic rhinosinusitis. PLoS One. 13(e0201595)2018.PubMed/NCBI View Article : Google Scholar

25 

Julien S, Puig I, Caretti E, Bonaventure J, Nelles L, van Roy F, Dargemont C, de Herreros AG, Bellacosa A and Larue L: Activation of NF-kappaB by Akt upregulates Snail expression and induces epithelium mesenchyme transition. Oncogene. 26:7445–7456. 2007.PubMed/NCBI View Article : Google Scholar

26 

Cano A, Pérez-Moreno MA, Rodrigo I, Locascio A, Blanco MJ, del Barrio MG, Portillo F and Nieto MA: The transcription factor snail controls epithelial-mesenchymal transitions by repressing E-cadherin expression. Nat Cell Biol. 2:76–83. 2000.PubMed/NCBI View Article : Google Scholar

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Li M, Li H, Yang S, Liao X, Zhao C and Wang F: MicroRNA‑29b participates in the epithelial‑mesenchymal transition of retinal pigment epithelial cells through p‑p65. Exp Ther Med 22: 868, 2021.
APA
Li, M., Li, H., Yang, S., Liao, X., Zhao, C., & Wang, F. (2021). MicroRNA‑29b participates in the epithelial‑mesenchymal transition of retinal pigment epithelial cells through p‑p65. Experimental and Therapeutic Medicine, 22, 868. https://doi.org/10.3892/etm.2021.10300
MLA
Li, M., Li, H., Yang, S., Liao, X., Zhao, C., Wang, F."MicroRNA‑29b participates in the epithelial‑mesenchymal transition of retinal pigment epithelial cells through p‑p65". Experimental and Therapeutic Medicine 22.2 (2021): 868.
Chicago
Li, M., Li, H., Yang, S., Liao, X., Zhao, C., Wang, F."MicroRNA‑29b participates in the epithelial‑mesenchymal transition of retinal pigment epithelial cells through p‑p65". Experimental and Therapeutic Medicine 22, no. 2 (2021): 868. https://doi.org/10.3892/etm.2021.10300
Copy and paste a formatted citation
x
Spandidos Publications style
Li M, Li H, Yang S, Liao X, Zhao C and Wang F: MicroRNA‑29b participates in the epithelial‑mesenchymal transition of retinal pigment epithelial cells through p‑p65. Exp Ther Med 22: 868, 2021.
APA
Li, M., Li, H., Yang, S., Liao, X., Zhao, C., & Wang, F. (2021). MicroRNA‑29b participates in the epithelial‑mesenchymal transition of retinal pigment epithelial cells through p‑p65. Experimental and Therapeutic Medicine, 22, 868. https://doi.org/10.3892/etm.2021.10300
MLA
Li, M., Li, H., Yang, S., Liao, X., Zhao, C., Wang, F."MicroRNA‑29b participates in the epithelial‑mesenchymal transition of retinal pigment epithelial cells through p‑p65". Experimental and Therapeutic Medicine 22.2 (2021): 868.
Chicago
Li, M., Li, H., Yang, S., Liao, X., Zhao, C., Wang, F."MicroRNA‑29b participates in the epithelial‑mesenchymal transition of retinal pigment epithelial cells through p‑p65". Experimental and Therapeutic Medicine 22, no. 2 (2021): 868. https://doi.org/10.3892/etm.2021.10300
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team