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Jianpiyiqi formula ameliorates chronic atrophic gastritis in rats by modulating the Wnt/β‑catenin signaling pathway

  • Authors:
    • Zhanpeng Yan
    • Tingting Xu
    • Yuxuan Xu
    • Wanzhen  Chen
    • Zhentao An
    • Fangshi Zhu
  • View Affiliations / Copyright

    Affiliations: Clinical Research Department of Chinese and Western Medicine, Affiliated Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing, Jiangsu 210028, P.R. China
    Copyright: © Yan et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 878
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    Published online on: June 15, 2021
       https://doi.org/10.3892/etm.2021.10310
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Abstract

Jianpiyiqi formula is a Traditional Chinese Medicine (TCM) prescription and is used for the clinical treatment of patients with chronic atrophic gastritis (CAG). The aim of the present study was to examine the underlying mechanisms of Jianpiyiqi formula treatment for CAG via the Wnt/β‑catenin signaling pathway. The high‑performance liquid chromatography (HPLC) chromatogram of Jianpiyiqi formula was constructed. A CAG rat model induced by N‑methyl‑N'‑nitro‑N‑nitrosoguanidine and ranitidine was established. The body weight and food intake of the rats was recorded and rat gastric morphology was visually examined. Pathological analysis of rat gastric tissue was also performed. The levels of gastrin (GAS), pepsin (PP), somatostatin (SS) and prostaglandin E2 (PGE2) in rat serum were detected using ELISAs. The expression levels of proteins and genes associated with the Wnt/β‑catenin signaling pathway were measured via immunohistochemistry and reverse transcription‑quantitative PCR. The HPLC chromatogram of Jianpiyiqi formula was determined and as active components, liquiritin and hesperidin were identified from the chromatogram. Compared with the blank group, the body weight and feed intake of the rats were decreased, and gastric mucosal atrophy and inflammation appeared in the model group. Treatment with Jianpiyiqi formula increased the body weight and feed intake of the rats, as well as relieved the gastric atrophy and inflammation. The contents of GAS, PP, SS and PGE2 were significantly reduced in the model group compared with the blank group. Jianpiyiqi formula significantly increased GAS, PP, SS and PGE2 levels in serum of rats with CAG. In the model group, Wnt1, β‑catenin and cyclin D1 protein expression levels were increased, and glycogen synthase kinase‑3β (GSK‑3β) protein expression levels were decreased. Jianpiyiqi formula decreased the protein expression levels of Wnt1, β‑catenin and cyclin D1 and increased the protein expression levels of GSK‑3β. Compared with the blank group, the mRNA expression levels of Wnt1, Wnt5a, β‑catenin, cyclin D1 and MMP7 were upregulated, and the mRNA expression levels of GSK‑3β were downregulated in the model group. Treatment with Jianpiyiqi formula downregulated the mRNA expression levels of Wnt1, Wnt5a, β‑catenin, cyclin D1 and MMP7 and upregulated the mRNA expression levels of GSK‑3β. All of the experimental results indicated that Jianpiyiqi formula exerted a therapeutic effect on rats with CAG and inhibited the activation of the Wnt/β‑catenin signaling pathway. Thus, Jianpiyiqi formula, as an effective TCM prescription for treating patients with CAG, may be more widely used in the clinic.
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Copy and paste a formatted citation
Spandidos Publications style
Yan Z, Xu T, Xu Y, Chen W, An Z and Zhu F: Jianpiyiqi formula ameliorates chronic atrophic gastritis in rats by modulating the Wnt/β‑catenin signaling pathway. Exp Ther Med 22: 878, 2021.
APA
Yan, Z., Xu, T., Xu, Y., Chen, W., An, Z., & Zhu, F. (2021). Jianpiyiqi formula ameliorates chronic atrophic gastritis in rats by modulating the Wnt/β‑catenin signaling pathway. Experimental and Therapeutic Medicine, 22, 878. https://doi.org/10.3892/etm.2021.10310
MLA
Yan, Z., Xu, T., Xu, Y., Chen, W., An, Z., Zhu, F."Jianpiyiqi formula ameliorates chronic atrophic gastritis in rats by modulating the Wnt/β‑catenin signaling pathway". Experimental and Therapeutic Medicine 22.2 (2021): 878.
Chicago
Yan, Z., Xu, T., Xu, Y., Chen, W., An, Z., Zhu, F."Jianpiyiqi formula ameliorates chronic atrophic gastritis in rats by modulating the Wnt/β‑catenin signaling pathway". Experimental and Therapeutic Medicine 22, no. 2 (2021): 878. https://doi.org/10.3892/etm.2021.10310
Copy and paste a formatted citation
x
Spandidos Publications style
Yan Z, Xu T, Xu Y, Chen W, An Z and Zhu F: Jianpiyiqi formula ameliorates chronic atrophic gastritis in rats by modulating the Wnt/β‑catenin signaling pathway. Exp Ther Med 22: 878, 2021.
APA
Yan, Z., Xu, T., Xu, Y., Chen, W., An, Z., & Zhu, F. (2021). Jianpiyiqi formula ameliorates chronic atrophic gastritis in rats by modulating the Wnt/β‑catenin signaling pathway. Experimental and Therapeutic Medicine, 22, 878. https://doi.org/10.3892/etm.2021.10310
MLA
Yan, Z., Xu, T., Xu, Y., Chen, W., An, Z., Zhu, F."Jianpiyiqi formula ameliorates chronic atrophic gastritis in rats by modulating the Wnt/β‑catenin signaling pathway". Experimental and Therapeutic Medicine 22.2 (2021): 878.
Chicago
Yan, Z., Xu, T., Xu, Y., Chen, W., An, Z., Zhu, F."Jianpiyiqi formula ameliorates chronic atrophic gastritis in rats by modulating the Wnt/β‑catenin signaling pathway". Experimental and Therapeutic Medicine 22, no. 2 (2021): 878. https://doi.org/10.3892/etm.2021.10310
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