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LGR5 enhances the osteoblastic differentiation of MC3T3‑E1 cells through the Wnt/β‑catenin pathway

  • Authors:
    • Wei Yu
    • Chao-Ran Xie
    • Fan-Cheng Chen
    • Pei Cheng
    • Lei Yang
    • Xiao-Yun Pan
  • View Affiliations / Copyright

    Affiliations: Department of Orthopedic Surgery, The Second Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang 325000, P.R. China, Institute of Advanced Materials for Nano‑Bio Applications, School of Ophthalmology and Optometry, Wenzhou Medical University, Wenzhou, Zhejiang 325000, P.R. China, Department of Orthopedic Surgery, Zhongshan Hospital, Fudan University, Shanghai 200433, P.R. China
    Copyright: © Yu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 889
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    Published online on: June 17, 2021
       https://doi.org/10.3892/etm.2021.10321
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Abstract

Leucine‑rich repeat‑containing G‑protein coupled receptor 5 (LGR5) is a Wnt‑associated gene that contributes to cell proliferation and self‑renewal in various organs. LGR5 is expressed in Ewing sarcoma, and LGR5‑overexpressing mesenchymal stem cells promote fracture healing. However, the effects of LGR5 on osteoblastic differentiation remain unclear. The aim of the present study was to explore the function of LGR5 in osteoblastic differentiation. LGR5 was overexpressed or knocked down in the MC3T3‑E1 pre‑osteoblastic cell line via lentiviral transfection and its function in osteoblastic differentiation was investigated. The mRNA expression levels of the osteoblast differentiation markers alkaline phosphatase (ALP), osteocalcin and collagen type I a1 were determined, and ALP and Alizarin red staining were performed. In addition, the effects of LGR5 modulation on β‑catenin and the expression of target genes in the Wnt pathway were investigated. The results revealed that the overexpression of LGR5 promoted osteoblastic differentiation. This was associated with enhancement of the stability of β‑catenin and its levels in the cell nucleus, which enabled it to activate Wnt signaling. By contrast, the inhibition of LGR5 decreased the osteogenic capacity of MC3T3‑E1 cells. These results indicate that LGR5 is a positive regulator of osteoblastic differentiation, whose effects are mediated through the Wnt/β‑catenin signaling pathway. This suggests suggesting that the regulation of LGR5/Wnt/β‑catenin signaling has potential as a therapy for osteoporosis.
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Copy and paste a formatted citation
Spandidos Publications style
Yu W, Xie C, Chen F, Cheng P, Yang L and Pan X: LGR5 enhances the osteoblastic differentiation of MC3T3‑E1 cells through the Wnt/β‑catenin pathway. Exp Ther Med 22: 889, 2021.
APA
Yu, W., Xie, C., Chen, F., Cheng, P., Yang, L., & Pan, X. (2021). LGR5 enhances the osteoblastic differentiation of MC3T3‑E1 cells through the Wnt/β‑catenin pathway. Experimental and Therapeutic Medicine, 22, 889. https://doi.org/10.3892/etm.2021.10321
MLA
Yu, W., Xie, C., Chen, F., Cheng, P., Yang, L., Pan, X."LGR5 enhances the osteoblastic differentiation of MC3T3‑E1 cells through the Wnt/β‑catenin pathway". Experimental and Therapeutic Medicine 22.2 (2021): 889.
Chicago
Yu, W., Xie, C., Chen, F., Cheng, P., Yang, L., Pan, X."LGR5 enhances the osteoblastic differentiation of MC3T3‑E1 cells through the Wnt/β‑catenin pathway". Experimental and Therapeutic Medicine 22, no. 2 (2021): 889. https://doi.org/10.3892/etm.2021.10321
Copy and paste a formatted citation
x
Spandidos Publications style
Yu W, Xie C, Chen F, Cheng P, Yang L and Pan X: LGR5 enhances the osteoblastic differentiation of MC3T3‑E1 cells through the Wnt/β‑catenin pathway. Exp Ther Med 22: 889, 2021.
APA
Yu, W., Xie, C., Chen, F., Cheng, P., Yang, L., & Pan, X. (2021). LGR5 enhances the osteoblastic differentiation of MC3T3‑E1 cells through the Wnt/β‑catenin pathway. Experimental and Therapeutic Medicine, 22, 889. https://doi.org/10.3892/etm.2021.10321
MLA
Yu, W., Xie, C., Chen, F., Cheng, P., Yang, L., Pan, X."LGR5 enhances the osteoblastic differentiation of MC3T3‑E1 cells through the Wnt/β‑catenin pathway". Experimental and Therapeutic Medicine 22.2 (2021): 889.
Chicago
Yu, W., Xie, C., Chen, F., Cheng, P., Yang, L., Pan, X."LGR5 enhances the osteoblastic differentiation of MC3T3‑E1 cells through the Wnt/β‑catenin pathway". Experimental and Therapeutic Medicine 22, no. 2 (2021): 889. https://doi.org/10.3892/etm.2021.10321
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