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At elevated temperatures, heat shock protein genes show altered ratios of different RNAs and expression of new RNAs, including several novel HSPB1 mRNAs encoding HSP27 protein isoforms

  • Authors:
    • Xia Gao
    • Keyin Zhang
    • Haiyan Zhou
    • Lucas Zellmer
    • Chengfu Yuan
    • Hai Huang
    • Dezhong Joshua Liao
  • View Affiliations / Copyright

    Affiliations: Department of Pathology, Guizhou Medical University Hospital, Guiyang, Guizhou 550004, P.R. China, Clinical Research Center, Guizhou Medical University Hospital, Guiyang, Guizhou 550004, P.R. China, Masonic Cancer Center, University of Minnesota, Minneapolis, MN 55455, USA, Department of Biochemistry, China Three Gorges University, Yichang, Hubei 443002, P.R. China, Center for Clinical Laboratories, Guizhou Medical University Hospital, Guiyang, Guizhou 550004, P.R. China, Key Lab of Endemic and Ethnic Diseases of The Ministry of Education of China in Guizhou Medical University, Guiyang, Guizhou 550004, P.R. China
    Copyright: © Gao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 900
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    Published online on: June 24, 2021
       https://doi.org/10.3892/etm.2021.10332
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Abstract

Heat shock proteins (HSP) serve as chaperones to maintain the physiological conformation and function of numerous cellular proteins when the ambient temperature is increased. To determine how accurate the general assumption that HSP gene expression is increased in febrile situations is, the RNA levels of the HSF1 (heat shock transcription factor 1) gene and certain HSP genes were determined in three cell lines cultured at 37˚C or 39˚C for three days. At 39˚C, the expression of HSF1, HSPB1, HSP90AA1 and HSP70A1L genes demonstrated complex changes in the ratios of expression levels between different RNA variants of the same gene. Several older versions of the RNAs of certain HSP genes that have been replaced by a newer version in the National Center for Biotechnology Information database were also detected, indicating that the older versions are actually RNA variants of these genes. The present study cloned four new RNA variants of the HSP27‑encoding HSPB1 gene, which together encode three short HSP27 peptides. Reanalysis of the proteomics data from our previous studies also demonstrated that proteins from certain HSP genes could be detected simultaneously at multiple positions using SDS‑PAGE, suggesting that these genes may engender multiple protein isoforms. These results collectively suggested that, besides increasing their expression, certain HSP and associated genes also use alternative transcription start sites to produce multiple RNA transcripts and use alternative splicing of a transcript to produce multiple mature RNAs, as important mechanisms for responding to an increased ambient temperature in vitro.
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Copy and paste a formatted citation
Spandidos Publications style
Gao X, Zhang K, Zhou H, Zellmer L, Yuan C, Huang H and Liao DJ: At elevated temperatures, heat shock protein genes show altered ratios of different RNAs and expression of new RNAs, including several novel HSPB1 mRNAs encoding HSP27 protein isoforms. Exp Ther Med 22: 900, 2021.
APA
Gao, X., Zhang, K., Zhou, H., Zellmer, L., Yuan, C., Huang, H., & Liao, D.J. (2021). At elevated temperatures, heat shock protein genes show altered ratios of different RNAs and expression of new RNAs, including several novel HSPB1 mRNAs encoding HSP27 protein isoforms. Experimental and Therapeutic Medicine, 22, 900. https://doi.org/10.3892/etm.2021.10332
MLA
Gao, X., Zhang, K., Zhou, H., Zellmer, L., Yuan, C., Huang, H., Liao, D. J."At elevated temperatures, heat shock protein genes show altered ratios of different RNAs and expression of new RNAs, including several novel HSPB1 mRNAs encoding HSP27 protein isoforms". Experimental and Therapeutic Medicine 22.2 (2021): 900.
Chicago
Gao, X., Zhang, K., Zhou, H., Zellmer, L., Yuan, C., Huang, H., Liao, D. J."At elevated temperatures, heat shock protein genes show altered ratios of different RNAs and expression of new RNAs, including several novel HSPB1 mRNAs encoding HSP27 protein isoforms". Experimental and Therapeutic Medicine 22, no. 2 (2021): 900. https://doi.org/10.3892/etm.2021.10332
Copy and paste a formatted citation
x
Spandidos Publications style
Gao X, Zhang K, Zhou H, Zellmer L, Yuan C, Huang H and Liao DJ: At elevated temperatures, heat shock protein genes show altered ratios of different RNAs and expression of new RNAs, including several novel HSPB1 mRNAs encoding HSP27 protein isoforms. Exp Ther Med 22: 900, 2021.
APA
Gao, X., Zhang, K., Zhou, H., Zellmer, L., Yuan, C., Huang, H., & Liao, D.J. (2021). At elevated temperatures, heat shock protein genes show altered ratios of different RNAs and expression of new RNAs, including several novel HSPB1 mRNAs encoding HSP27 protein isoforms. Experimental and Therapeutic Medicine, 22, 900. https://doi.org/10.3892/etm.2021.10332
MLA
Gao, X., Zhang, K., Zhou, H., Zellmer, L., Yuan, C., Huang, H., Liao, D. J."At elevated temperatures, heat shock protein genes show altered ratios of different RNAs and expression of new RNAs, including several novel HSPB1 mRNAs encoding HSP27 protein isoforms". Experimental and Therapeutic Medicine 22.2 (2021): 900.
Chicago
Gao, X., Zhang, K., Zhou, H., Zellmer, L., Yuan, C., Huang, H., Liao, D. J."At elevated temperatures, heat shock protein genes show altered ratios of different RNAs and expression of new RNAs, including several novel HSPB1 mRNAs encoding HSP27 protein isoforms". Experimental and Therapeutic Medicine 22, no. 2 (2021): 900. https://doi.org/10.3892/etm.2021.10332
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