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Clinical impact of copy number variation changes in bladder cancer samples

  • Authors:
    • Victoria Spasova
    • Boris Mladenov
    • Simeon Rangelov
    • Zora Hammoudeh
    • Desislava Nesheva
    • Dimitar Serbezov
    • Rada Staneva
    • Savina Hadjidekova
    • Mihail Ganev
    • Lubomir Balabanski
    • Radoslava Vazharova
    • Chavdar Slavov
    • Draga Toncheva
    • Olga Antonova
  • View Affiliations / Copyright

    Affiliations: Department of Medical Genetics, Medical University‑Sofia, 1431 Sofia, Bulgaria, Department of Urology, UMBALSM N.I. Pirogov, 1606 Sofia, Bulgaria, Department of Urology, Tsaritsa Yoanna University Hospital, 1527 Sofia, Bulgaria, Medical Genetics Laboratory, GARH Malinov, 1680 Sofia, Bulgaria
    Copyright: © Spasova et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 901
    |
    Published online on: June 24, 2021
       https://doi.org/10.3892/etm.2021.10333
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Abstract

The aim of the present study was to detect copy number variations (CNVs) related to tumour progression and metastasis of urothelial carcinoma through whole‑genome scanning. A total of 30 bladder cancer samples staged from pTa to pT4 were included in the study. DNA was extracted from freshly frozen tissue via standard phenol‑chloroform extraction and CNV analysis was performed on two alternative platforms (CytoChip Oligo aCGH, 4x44K and Infinium OncoArray‑500K BeadChip; Illumina, Inc.). Data were analysed with BlueFuse Multi software and Karyostudio, respectively. The results highlight the role of genomic imbalances in regions containing genes with metastatic and proliferative potential for tumour invasion. A high level of genomic instability in uroepithelial tumours was observed and a total of 524 aberrations, including 175 losses and 349 gains, were identified. The most prevalent genetic imbalances affected the following regions: 1p, 1q, 2q, 4p, 4q, 5p, 5q, 6p, 6q, 7q, 8q, 9p, 9q, 10p, 10q, 11q, 13q and 17q. High‑grade tumours more frequently harboured genomic imbalances (n=227) than low‑grade tumours (n=103). A total of 36 CNVs in high‑grade bladder tumours were detected in chromosomes 1‑5, 8‑11, 14, 17, 19 and 20. Furthermore, five loss of heterozygosity variants containing 176 genes were observed in high‑grade bladder cancer and may be used as potential targets for precision therapy. Revealing specific chromosomal regions related to the metastatic potential of uroepithelial tumours may lay a foundation for implementing molecular CNV profiling of bladder tumours as part of a routine progression risk estimation strategy, thus expanding the personalized therapeutic approach.
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1 

Conconi D, Panzeri E, Redaelli S, Bovo G, Viganò P, Strada G, Dalprà L and Bentivegna A: Chromosomal imbalances in human bladder urothelial carcinoma: Similarities and differences between biopsy samples and cancer stem-like cells. BMC Cancer. 14(646)2014.PubMed/NCBI View Article : Google Scholar

2 

Kawanishi H, Takahashi T, Ito M, Matsui Y, Watanabe J, Ito N, Kamoto T, Kadowaki T, Tsujimoto G, Imoto I, et al: Genetic analysis of multifocal superficial urothelial cancers by array-based comparative genomic hybridisation. Br J Cancer. 97:260–266. 2007.PubMed/NCBI View Article : Google Scholar

3 

Kallioniemi A, Kallioniemi OP, Citro G, Sauter G, DeVries S, Kerschmann R, Caroll P and Waldman F: Identification of gains and losses of DNA sequences in primary bladder cancer by comparative genomic hybridization. Genes Chromosomes Cancer. 12:213–219. 1995.PubMed/NCBI View Article : Google Scholar

4 

Sauter G: Rudolf Virchow Prize 1997. Molecular cytogenetic analysis of superficial urothelial cancer of the bladder. Verh Dtsch Ges Pathol. 81:18–27. 1997.PubMed/NCBI(In German).

5 

Voorter C, Joos S, Bringuier PP, Vallinga M, Poddighe P, Schalken J, du Manoir S, Ramaekers F, Lichter P and Hopman A: Detection of chromosomal imbalances in transitional cell carcinoma of the bladder by comparative genomic hybridization. Am J Pathol. 146:1341–1354. 1995.PubMed/NCBI

6 

Zaharieva BM, Simon R, Diener PA, Ackermann D, Maurer R, Alund G, Knönagel H, Rist M, Wilber K, Hering F, et al: High-throughput tissue microarray analysis of 11q13 gene amplification (CCND1, FGF3, FGF4, EMS1) in urinary bladder cancer. J Pathol. 201:603–608. 2003.PubMed/NCBI View Article : Google Scholar

7 

Huang WC, Taylor S, Nguyen TB, Tomaszewski JE, Libertino JA, Malkowicz SB and McGarvey TW: KIAA1096, a gene on chromosome 1q, is amplified and overexpressed in bladder cancer. DNA Cell Biol. 21:707–715. 2002.PubMed/NCBI View Article : Google Scholar

8 

Veltman JA, Fridlyand J, Pejavar S, Olshen AB, Korkola JE, DeVries S, Carroll P, Kuo WL, Pinkel D, Albertson D, et al: Array-based comparative genomic hybridization for genome-wide screening of DNA copy number in bladder tumors. Cancer Res. 63:2872–2880. 2003.PubMed/NCBI

9 

Bellmunt J, Selvarajah S, Rodig S, Salido M, de Muga S, Costa I, Bellosillo B, Werner L, Mullane S, Fay AP, et al: Identification of ALK gene alterations in urothelial carcinoma. PLoS One. 9(e103325)2014.PubMed/NCBI View Article : Google Scholar

10 

Scaravilli M, Asero P, Tammela TL, Visakorpi T and Saramäki OR: Mapping of the chromosomal amplification 1p21-22 in bladder cancer. BMC Res Notes. 7(547)2014.PubMed/NCBI View Article : Google Scholar

11 

Weilandt M, Koch A, Rieder H, Deenen R, Schwender H, Niegisch G and Schulz WA: Target genes of recurrent chromosomal amplification and deletion in urothelial carcinoma. Cancer Genomics Proteomics. 11:141–153. 2014.PubMed/NCBI

12 

Ewald JA, Downs TM, Cetnar JP and Ricke WA: Expression microarray meta-analysis identifies genes associated with Ras/MAPK and related pathways in progression of muscle-invasive bladder transition cell carcinoma. PLoS One. 8(e55414)2013.PubMed/NCBI View Article : Google Scholar

13 

Hussain SA, Palmer DH, Syn WK, Sacco JJ, Greensmith RM, Elmetwali T, Aachi V, Lloyd BH, Jithesh PV, Arrand J, et al: Gene expression profiling in bladder cancer identifies potential therapeutic targets. Int J Oncol. 50:1147–1159. 2017.PubMed/NCBI View Article : Google Scholar

14 

Mengual L, Burset M, Ars E, Lozano JJ, Villavicencio H, Ribal MJ and Alcaraz A: DNA microarray expression profiling of bladder cancer allows identification of noninvasive diagnostic markers. J Urol. 182:741–748. 2009.PubMed/NCBI View Article : Google Scholar

15 

Sanchez-Carbayo M: Use of high-throughput DNA microarrays to identify biomarkers for bladder cancer. Clin Chem. 49:23–31. 2003.PubMed/NCBI View Article : Google Scholar

16 

de Ravel TJ, Devriendt K, Fryns JP and Vermeesch JR: What's new in karyotyping? The move towards array comparative genomic hybridisation (CGH). Eur J Pediatr. 166:637–643. 2007.PubMed/NCBI View Article : Google Scholar

17 

Letasiova S, Medve'ova A, Sovcikova A, Dušinská M, Volkovová K, Mosoiu C and Bartonová A: Bladder cancer, a review of the environmental risk factors. Environ Health. 11 Suppl 1(Suppl 1)(S11)2012.PubMed/NCBI View Article : Google Scholar

18 

Minner S, Kilgue A, Stahl P, Weikert S, Rink M, Dahlem R, Fisch M, Höppner W, Wagner W, Bokemeyer C, et al: Y chromosome loss is a frequent early event in urothelial bladder cancer. Pathology. 42:356–359. 2010.PubMed/NCBI View Article : Google Scholar

19 

Panani AD and Roussos C: Sex chromosome abnormalities in bladder cancer: Y polysomies are linked to PT1-grade III transitional cell carcinoma. Anticancer Res. 26(1A):319–323. 2006.PubMed/NCBI

20 

Forsberg LA: Loss of chromosome Y (LOY) in blood cells is associated with increased risk for disease and mortality in aging men. Hum Genet. 136:657–663. 2017.PubMed/NCBI View Article : Google Scholar

21 

Lopez V, Gonzalez-Peramato P, Suela J, Serrano A, Algaba F, Cigudosa JC, Vidal A, Bellmunt J, Heredero O and Sánchez-Carbayo M: Identification of prefoldin amplification (1q23.3-q24.1) in bladder cancer using comparative genomic hybridization (CGH) arrays of urinary DNA. J Transl Med. 11(182)2013.PubMed/NCBI View Article : Google Scholar

22 

Huang Y, Li G, Wang K, Mu Z, Xie Q, Qu H, Lv H and Hu B: Collagen Type VI Alpha 3 Chain promotes epithelial-mesenchymal transition in bladder cancer cells via transforming growth factor β (TGF-β)/Smad pathway. Med Sci Monit. 24:5346–5354. 2018.PubMed/NCBI View Article : Google Scholar

23 

Liang Y, Luo H, Zhang H, Dong Y and Bao Y: Oncogene Delta/Notch-Like EGF-Related receptor promotes cell proliferation, invasion, and migration in hepatocellular carcinoma and predicts a poor prognosis. Cancer Biother Radiopharm. 33:380–386. 2018.PubMed/NCBI View Article : Google Scholar

24 

Deng S, He SY, Zhao P and Zhang P: The role of oncostatin M receptor gene polymorphisms in bladder cancer. World J Surg Oncol. 17(30)2019.PubMed/NCBI View Article : Google Scholar

25 

Sun J, Zhang H, Tao D, Xie F, Liu F, Gu C, Wang M, Wang L, Jiang G, Wang Z and Xiao X: CircCDYL inhibits the expression of C-MYC to suppress cell growth and migration in bladder cancer. Artif Cells Nanomed Biotechnol. 47:1349–1356. 2019.PubMed/NCBI View Article : Google Scholar

26 

Chen Y, Peng Y, Xu Z, Ge B, Xiang X, Zhang T, Gao L, Shi H, Wang C and Huang J: Knockdown of lncRNA SNHG7 inhibited cell proliferation and migration in bladder cancer through activating Wnt/β-catenin pathway. Pathol Res Pract. 215:302–307. 2019.PubMed/NCBI View Article : Google Scholar

27 

Daizumoto K, Yoshimaru T, Matsushita Y, Fukawa T, Uehara H, Ono M, Komatsu M, Kanayama HO and Katagiri T: A DDX31/Mutant-p53/EGFR Axis promotes multistep progression of Muscle-Invasive bladder cancer. Cancer Res. 78:2233–2247. 2018.PubMed/NCBI View Article : Google Scholar

28 

Wu L, Chaffee KG, Parker AS, Sicotte H and Petersen GM: Zinc transporter genes and urological cancers: Integrated analysis suggests a role for ZIP11 in bladder cancer. Tumour Biol. 36:7431–7437. 2015.PubMed/NCBI View Article : Google Scholar

29 

Kim YW, Yoon HY, Seo SP, Lee SK, Kang HW, Kim WT, Bang HJ, Ryu DH, Yun SJ, Lee SC, et al: Clinical implications and prognostic values of prostate cancer susceptibility candidate methylation in primary nonmuscle invasive bladder cancer. Dis Markers. 2015(402963)2015.PubMed/NCBI View Article : Google Scholar

30 

Chen Z, Zhou L, Wang L, Kazobinka G, Zhang X, Han X, Li B and Hou T: HBO1 promotes cell proliferation in bladder cancer via activation of Wnt/β-catenin signaling. Mol Carcinog. 57:12–21. 2018.PubMed/NCBI View Article : Google Scholar

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Copy and paste a formatted citation
Spandidos Publications style
Spasova V, Mladenov B, Rangelov S, Hammoudeh Z, Nesheva D, Serbezov D, Staneva R, Hadjidekova S, Ganev M, Balabanski L, Balabanski L, et al: Clinical impact of copy number variation changes in bladder cancer samples. Exp Ther Med 22: 901, 2021.
APA
Spasova, V., Mladenov, B., Rangelov, S., Hammoudeh, Z., Nesheva, D., Serbezov, D. ... Antonova, O. (2021). Clinical impact of copy number variation changes in bladder cancer samples. Experimental and Therapeutic Medicine, 22, 901. https://doi.org/10.3892/etm.2021.10333
MLA
Spasova, V., Mladenov, B., Rangelov, S., Hammoudeh, Z., Nesheva, D., Serbezov, D., Staneva, R., Hadjidekova, S., Ganev, M., Balabanski, L., Vazharova, R., Slavov, C., Toncheva, D., Antonova, O."Clinical impact of copy number variation changes in bladder cancer samples". Experimental and Therapeutic Medicine 22.2 (2021): 901.
Chicago
Spasova, V., Mladenov, B., Rangelov, S., Hammoudeh, Z., Nesheva, D., Serbezov, D., Staneva, R., Hadjidekova, S., Ganev, M., Balabanski, L., Vazharova, R., Slavov, C., Toncheva, D., Antonova, O."Clinical impact of copy number variation changes in bladder cancer samples". Experimental and Therapeutic Medicine 22, no. 2 (2021): 901. https://doi.org/10.3892/etm.2021.10333
Copy and paste a formatted citation
x
Spandidos Publications style
Spasova V, Mladenov B, Rangelov S, Hammoudeh Z, Nesheva D, Serbezov D, Staneva R, Hadjidekova S, Ganev M, Balabanski L, Balabanski L, et al: Clinical impact of copy number variation changes in bladder cancer samples. Exp Ther Med 22: 901, 2021.
APA
Spasova, V., Mladenov, B., Rangelov, S., Hammoudeh, Z., Nesheva, D., Serbezov, D. ... Antonova, O. (2021). Clinical impact of copy number variation changes in bladder cancer samples. Experimental and Therapeutic Medicine, 22, 901. https://doi.org/10.3892/etm.2021.10333
MLA
Spasova, V., Mladenov, B., Rangelov, S., Hammoudeh, Z., Nesheva, D., Serbezov, D., Staneva, R., Hadjidekova, S., Ganev, M., Balabanski, L., Vazharova, R., Slavov, C., Toncheva, D., Antonova, O."Clinical impact of copy number variation changes in bladder cancer samples". Experimental and Therapeutic Medicine 22.2 (2021): 901.
Chicago
Spasova, V., Mladenov, B., Rangelov, S., Hammoudeh, Z., Nesheva, D., Serbezov, D., Staneva, R., Hadjidekova, S., Ganev, M., Balabanski, L., Vazharova, R., Slavov, C., Toncheva, D., Antonova, O."Clinical impact of copy number variation changes in bladder cancer samples". Experimental and Therapeutic Medicine 22, no. 2 (2021): 901. https://doi.org/10.3892/etm.2021.10333
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