Cell‑penetrating heme oxygenase‑1 in the therapy of atopic dermatitis in mice

Tang,Fang; Ma,Xueqing; Sun,Jiayu; Ru,Minghui; Qian,Tiansheng; Ji,Wengjing; Qian,Sifan; Li,Hua

doi:10.3892/etm.2021.10373

Cell‑penetrating heme oxygenase‑1 in the therapy of atopic dermatitis in mice

Authors:
- Fang Tang
- Xueqing Ma
- Jiayu Sun
- Minghui Ru
- Tiansheng Qian
- Wengjing Ji
- Sifan Qian
- Hua Li
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Affiliations: School of Medicine, Huzhou University, Huzhou, Zhejiang 313000, P.R. China
Published online on: July 1, 2021 https://doi.org/10.3892/etm.2021.10373
Article Number: 941
Copyright: © Tang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Atopic dermatitis (AD), also referred to as atopic eczema, is a long‑term inflammatory condition that is characterized by itchy, red, swollen and cracked skin. Accumulating evidence suggests that AD is caused by genetic factors, environmental exposure and immune system dysfunction; however, its underlying molecular mechanism remains unclear. Current treatment strategies aim to decrease the severity and frequency of flares. Heme oxygenase‑1 (HO‑1) is a nuclear factor erythroid 2‑related factor 2 (Nrf2)‑regulated gene that plays crucial roles against stress, inflammation and oxidation, and exerts cytoprotective effects. Previous studies have reported that treatment of AD induces high expression levels of HO‑1 and Nrf2, indicating that HO‑1 may play an important role in the treatment of AD. The present study constructed the recombinant protein, cell‑penetrating peptide‑HO‑1 (CPP‑HO‑1), which was expressed in Escherichia coli and isolated with a 6xHis‑tag using HiTrap His column (1 ml). AD was established using 4‑dinitrochlorobenzene (DNCB) in mice. It was observed that the CPP‑HO‑1 fusion protein decreased the severity of AD, inhibited scratching in mice and decreased skin inflammation. Taken together, the results of the present study suggested that the CPP‑HO‑1 fusion protein may play a protective role against DNCB‑induced AD in mice.

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