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Icariin inhibits oral squamous cell carcinoma cell proliferation and induces apoptosis via inhibiting the NF‑κB and PI3K/AKT pathways

  • Authors:
    • Ling Sun
    • Jing Zhang
  • View Affiliations / Copyright

    Affiliations: Department of Pharmacy, Jiangsu Vocational College of Medicine, Yancheng, Jiangsu 224005, P.R. China, Department of Hematology, Yancheng No. 1 People's Hospital, Yancheng, Jiangsu 224006, P.R. China
    Copyright: © Sun et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 942
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    Published online on: July 1, 2021
       https://doi.org/10.3892/etm.2021.10374
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Abstract

Oral squamous cell carcinoma (OSCC), one of the most common types of human cancer, has a high mortality rate and a poor prognosis due to its high rates of recurrence and metastasis. In recent years, icariin (ICA) has been reported to play an important role in a variety of malignancies, such as gastric, colorectal, pancreatic and ovarian cancer. However, its role and mechanism in OSCC remains to be elucidated. The present study aimed to investigate the effect of ICA in OSCC cells and to reveal its underlying mechanisms. The OSCC cell lines SCC9 and Cal 27 were used to explore the effect of different concentrations of ICA on the biological behavior of OSCC cells. The effect of ICA on OSCC cell proliferation and apoptosis was determined using 3‑(4,5‑dimethylthiazol‑2‑yl)‑2,5‑diphenyl‑2H‑tetrazolium bromide and flow cytometric assays, respectively. Subsequently, the protein expression levels of caspase‑3 and cleaved‑caspase‑3 were detected using western blot analysis. Additionally, the protein and mRNA expression levels of nuclear factor‑κB (NF‑κB) and phosphatidylinositol‑3‑kinase (PI3K)/protein kinase B (AKT) signaling pathway‑related factors were determined using western blot analysis and reverse transcription‑quantitative PCR, respectively. The results demonstrated that ICA inhibited OSCC cell proliferation and significantly increased the apoptosis rate in a dose‑dependent manner. In addition, treatment of OSCC cells with ICA upregulated the protein expression of cleaved‑caspase‑3 and increased the cleaved‑caspase‑3/caspase‑3 ratio. The protein expression levels of phosphorylated (p)‑p65, p‑PI3K and p‑AKT were decreased in OSCC cells treated with ICA. The aforementioned findings revealed that ICA could attenuate the proliferation of OSCC cells and induce apoptosis via inhibiting the NF‑κB and PI3K/AKT signaling pathways. Therefore, the current study provided a new insight into the clinical treatment of OSCC.
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1 

Qin L, Zhang G, Sheng H, Wang XL, Wang YX, Yeung KW, Griffith JF, Li ZR, Leung KS and Yao XS: Phytoestrogenic compounds for prevention of steroid-associated osteonecrosis. J Musculoskelet Neuronal Interact. 8:18–21. 2008.PubMed/NCBI

2 

Chen Y, Sun T, Wu J, Kalionis B, Zhang C, Yuan D, Huang J, Cai W, Fang H and Xia S: Icariin intervenes in cardiac inflammaging through upregulation of SIRT6 enzyme activity and inhibition of the NF-kappa B pathway. Biomed Res Int. 2015(895976)2015.PubMed/NCBI View Article : Google Scholar

3 

Carlsten M and Childs RW: Genetic manipulation of NK cells for cancer immunotherapy: Techniques and clinical implications. Front Inmunol. 6(266)2015.PubMed/NCBI View Article : Google Scholar

4 

Wang Z, Zhang H, Dai L, Song T, Li P, Liu Y and Wang L: Arsenic trioxide and icariin show synergistic anti-leukemic activity. Cell Biochem Biophys. 73:213–219. 2015.PubMed/NCBI View Article : Google Scholar

5 

Huang S, Xie T and Liu W: Icariin inhibits the growth of human cervical cancer cells by inducing apoptosis and autophagy by targeting mTOR/PI3K/AKT signalling pathway. J BUON. 24:990–996. 2019.PubMed/NCBI

6 

Wang P, Zhang J, Xiong X, Yuan W, Qin S, Cao W, Dai L, Xie F, Li A and Liu Z: Icariin suppresses cell cycle transition and cell migration in ovarian cancer cells. Oncol Rep. 41:2321–2328. 2019.PubMed/NCBI View Article : Google Scholar

7 

Kim B, Seo JH, Lee KY and Park B: Icariin sensitizes human colon cancer cells to TRAIL-induced apoptosis via ERK-mediated upregulation of death receptors. Int J Oncol. 56:821–834. 2020.PubMed/NCBI View Article : Google Scholar

8 

Song L, Chen X, Mi L, Liu C, Zhu S, Yang T, Luo X, Zhang Q, Lu H and Liang X: Icariin-induced inhibition of SIRT6/NF-κB triggers redox mediated apoptosis and enhances anti-tumor immunity in triple-negative breast cancer. Cancer Sci. 111:4242–4256. 2020.PubMed/NCBI View Article : Google Scholar

9 

Ju ZY, Tang QM, Chen LL, et al: Axl inhibitor R428 induces autophagy of oral squamous cell carcinoma Cal27 cells. J Pract Stomatol. 35:2019.

10 

Hu XG, Qiu ZL, Zen JC, et al: Differential expression of microRNAs in human oral squamous cell carcinoma stem cells. J Pract Stomatol. 3:398–402. 2019.

11 

Schneider P, Schon M, Pletz N, Seitz CS, Liu N, Ziegelbauer K, Zachmann K, Emmert S and Schön MP: The novel PI3 kinase inhibitor, BAY80-6946, impairs melanoma growth in vivo and in vitro. Exp Dermatol. 23:579–584. 2014.PubMed/NCBI View Article : Google Scholar

12 

Liu YJ, Huang LL, Hao BH, Li H, Zhu S, Wang Q, Li R, Xu Y and Zhang X: Use of an osteoblast overload damage model to probe the effect of icariin on the proliferation, differentiation and mineralization of MC3T3-E1cells through the Wnt/β-catenin signalling pathway. Cell Physiol Biochem. 41:1605–1615. 2017.PubMed/NCBI View Article : Google Scholar

13 

Li W, Wang M, Wang LY, Ji S, Zhang J and Zhang C: Icariin synergizes with arsenic trioxide to suppress human hepatocellular carcinoma. Cell Biochem Biophys. 68:427–436. 2014.PubMed/NCBI View Article : Google Scholar

14 

Yang LJ, Wang YX, Guo H and Guo M: Synergistic anti-cancer effects of icariin and temozolomide in glioblastoma. Cell Biochem Biophys. 71:1379–1385. 2015.PubMed/NCBI View Article : Google Scholar

15 

Shi DB, Li XX, Zheng HT, Li DW, Cai GX, Peng JJ, Gu WL, Guan ZQ, Xu Y and Cai SJ: Icariin-mediated inhibition of NF-κB activity enhances the in vitro and in vivo antitu-mour effect of 5-fluorouracil in colorectal cancer. Cell Biochem Biophys. 69:523–530. 2014.PubMed/NCBI View Article : Google Scholar

16 

Xu W, Tao H and Zhang Q: Effect of Six1 gene on As2O3-induced oral squamous cell carcinoma cell apoptosis and ROS levels. J Clin Exp Medicine. (15):2019.

17 

Peng M and Pang C: MicroRNA-140-5p inhibits the tumorigenesis of oral squamous cell carcinoma by targeting p21-activated kinase 4. Cell Biol Int: Aug 8, 2019 (Epub ahead of print).

18 

Wang XM, Liu CM, Zhang CR, Xu XG and Fu SB: Functional significance of TGF-beta1 signal transduction pathway in oral squamous cell carcinoma. Zhonghua Zhong Liu Za Zhi. 31:28–32. 2009.PubMed/NCBI(In Chinese).

19 

Chen Z, He J, Xing X, Li P, Zhang W, Tong Z, Jing X, Li L, Liu D, Wu Q and Ju H: Mn12Ac inhibits the migration, invasion and epithelial-mesenchymal transition of lung cancer cells by downregulating the Wnt/β-catenin and PI3K/AKT signaling pathways. Oncol Lett. 16:3943–3948. 2018.PubMed/NCBI View Article : Google Scholar

20 

Xie J, Lin W, Huang L, Xu N, Xu A, Chen B, Watanabe M, Liu C and Huang P: Bufalin suppresses the proliferation and metastasis of renal cell carcinoma by inhibiting the PI3K/Akt/mTOR signaling pathway. Oncol Lett. 16:3867–3873. 2018.PubMed/NCBI View Article : Google Scholar

21 

Choi MS, Moon SM, Lee SA, Park BR, Kim JS, Kim DK, Kim YH and Kim CS: Adenosine induces intrinsic apoptosis via the PI3K/Akt/mTOR signaling pathway in human pharyngeal squamous carcinoma FaDu cells. Oncol Lett. 15:6489–6496. 2018.PubMed/NCBI View Article : Google Scholar

22 

Hao Y, Zhang C, Sun Y and Xu H: Licochalcone A inhibits cell proliferation, migration, and invasion through regulating the PI3K/AKT signaling pathway in oral squamous cell carcinoma. Onco Targets Ther. 12:4427–4435. 2019.PubMed/NCBI View Article : Google Scholar

23 

Fan QC, Tian H, Wang Y and Liu XB: Integrin-alpha5 promoted the progression of oral squamous cell carcinoma and modulated PI3K/AKT signaling pathway. Arch Oral Biol. 101:85–91. 2019.PubMed/NCBI View Article : Google Scholar

24 

Velu P, Vijayalakshmi A and Vinothkumar V: Inhibiting the PI3K/Akt, NF-kB signalling pathways with syringic acid for attenuating the development of oral squamous cell carcinoma cells SCC131. J Pharm Pharmacol. 72:1595–1606. 2020.PubMed/NCBI View Article : Google Scholar

25 

Ferlay J, Soerjomataram I, Dikshit R, Eser S, Mathers C, Rebelo M, Parkin DM, Forman D and Bray F: Cancer incidence and mortality worldwide: Sources, methods and major patterns in GLOBOCAN 2012. Int J Cancer. 136:E359–E386. 2015.PubMed/NCBI View Article : Google Scholar

26 

Galluzzi L and Rudqvist NP: Preface: More than two decades of modern tumor immunology. Methods Enzymol. 629:xxii–xlii. 2019.PubMed/NCBI View Article : Google Scholar

27 

Zhang X, Kang Z, Li Q, Zhang J, Cheng S, Chang H, Wang S, Cao S, Li T, Li J, et al: Antigen-adjuvant effects of icariin in enhancing tumor-specific immunity in mastocytoma-bearing DBA/2J mice. Biomed Pharmacother. 99:810–816. 2018.PubMed/NCBI View Article : Google Scholar

28 

Gao M and Li W: In vitro study on the inhibitory effect of icariin on human bladder cancer T24 cells. Shijiazhuang: Hebei Medical University 2010.

29 

Hao H, Zhang Q, Zhu H, Wen Y, Qiu D, Xiong J, Fu X, Wu Y, Meng K and Li J: Icaritin promotes tumor T-cell infiltration and induces antitumor immunity in mice. Eur J Immunol. 49:2235–2244. 2019.PubMed/NCBI View Article : Google Scholar

30 

Tan HL, Chan KG, Pusparajah P, Saokaew S, Duangjai A, Lee LH and Goh BH: Anti-cancer properties of the naturally occurring aphrodisiacs: Icariin and its derivatives. Front Pharmacol. 7(191)2016.PubMed/NCBI View Article : Google Scholar

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Copy and paste a formatted citation
Spandidos Publications style
Sun L and Zhang J: Icariin inhibits oral squamous cell carcinoma cell proliferation and induces apoptosis via inhibiting the NF‑κB and PI3K/AKT pathways. Exp Ther Med 22: 942, 2021.
APA
Sun, L., & Zhang, J. (2021). Icariin inhibits oral squamous cell carcinoma cell proliferation and induces apoptosis via inhibiting the NF‑κB and PI3K/AKT pathways. Experimental and Therapeutic Medicine, 22, 942. https://doi.org/10.3892/etm.2021.10374
MLA
Sun, L., Zhang, J."Icariin inhibits oral squamous cell carcinoma cell proliferation and induces apoptosis via inhibiting the NF‑κB and PI3K/AKT pathways". Experimental and Therapeutic Medicine 22.3 (2021): 942.
Chicago
Sun, L., Zhang, J."Icariin inhibits oral squamous cell carcinoma cell proliferation and induces apoptosis via inhibiting the NF‑κB and PI3K/AKT pathways". Experimental and Therapeutic Medicine 22, no. 3 (2021): 942. https://doi.org/10.3892/etm.2021.10374
Copy and paste a formatted citation
x
Spandidos Publications style
Sun L and Zhang J: Icariin inhibits oral squamous cell carcinoma cell proliferation and induces apoptosis via inhibiting the NF‑κB and PI3K/AKT pathways. Exp Ther Med 22: 942, 2021.
APA
Sun, L., & Zhang, J. (2021). Icariin inhibits oral squamous cell carcinoma cell proliferation and induces apoptosis via inhibiting the NF‑κB and PI3K/AKT pathways. Experimental and Therapeutic Medicine, 22, 942. https://doi.org/10.3892/etm.2021.10374
MLA
Sun, L., Zhang, J."Icariin inhibits oral squamous cell carcinoma cell proliferation and induces apoptosis via inhibiting the NF‑κB and PI3K/AKT pathways". Experimental and Therapeutic Medicine 22.3 (2021): 942.
Chicago
Sun, L., Zhang, J."Icariin inhibits oral squamous cell carcinoma cell proliferation and induces apoptosis via inhibiting the NF‑κB and PI3K/AKT pathways". Experimental and Therapeutic Medicine 22, no. 3 (2021): 942. https://doi.org/10.3892/etm.2021.10374
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