Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Experimental and Therapeutic Medicine
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-0981 Online ISSN: 1792-1015
Journal Cover
September-2021 Volume 22 Issue 3

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
September-2021 Volume 22 Issue 3

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article Open Access

Characteristics of mouse intestinal microbiota during acute liver injury and repair following 50% partial hepatectomy

  • Authors:
    • Yi Shao
    • Yuancong Jiang
    • Hui Li
    • Feng Zhang
    • Zhenhua Hu
    • Shusen Zheng
  • View Affiliations / Copyright

    Affiliations: Department of Hepatobiliary and Pancreatic Surgery, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang 310003, P.R. China, Key Laboratory of Organ Transplantation, Research Center for Diagnosis and Treatment of Hepatobiliary Diseases, Hangzhou, Zhejiang 310003, P.R. China
    Copyright: © Shao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 953
    |
    Published online on: July 5, 2021
       https://doi.org/10.3892/etm.2021.10385
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Dysbiosis of the gut microbiota has important roles in various diseases and pathological states of the host. However, the changes of the gut microbiota during partial hepatectomy (PH)‑induced acute liver injury have so far remained elusive. The present study investigated the gut microbiome and its related pathways following PH‑induced acute liver injury. A total of 50 male C57/BL6 mice were divided into a normal control (NC), sham‑operation and liver resection (LR) group (50% PH). Samples were collected at 3 and 14 days post‑operation to obtain specimens for the Sham3, Sham14, LR3 and LR14 groups (10 mice/group). Specimens of NC group (n=10) were obtained at the same time as those of Sham3 group. Alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were determined using an automatic chemical analyzer and the gut microbiota was assessed by 16S ribosomal RNA gene sequencing of small intestinal contents. The serum levels of ALT and AST in the LR3 group were significantly increased, while those in the LR14 group were decreased again to near‑normal levels. In the LR3 group, the operational taxonomic units, species richness (Chao1) and species diversity (Shannon and Simpson indices) were decreased, although without any significant difference. Furthermore, in the LR3 group, significant Cyanobacteria enrichment and Fusobacteria depletion compared with the NC and Sham3 groups was observed, while in the LR14 group, a significant depletion of the abundance of Verrucomicrobia, Chloroflexi and Deferribacteres compared to the LR3 group was obtained. The abundance of Firmicutes was increased in the LR3 group and decreased again in the LR14 group. However, the abundance of Bacteroidetes and Actinobacteria decreased in the LR3 group and increased again in the LR14 group. The alterations of the gut microbiota at the genus level were also revealed, as significant increases in Chloroplast, Curvibacter, Pelomonas, Ruminococcaceae UCG‑005 and Blautia and a sharp decrease in Akkermansia and Eubacterium coprostanoligenes were caused by acute liver injury. Furthermore, functional metagenome prediction was performed by Phylogenetic Investigation of Communities by Reconstruction of Unobserved States based on the Greengenes database, revealing alterations in signal transduction, transcription and cell motility, as well as metabolism of amino acids, lipids, glucose, cofactors and terpenoids, and xenobiotics pathways. An improved understanding of the structural and functional changes of the gut microbiota following 50% PH‑induced acute liver injury and repair may provide novel strategies for the recovery of hosts undergoing hepatectomy.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Figure 6

Figure 7

View References

1 

Usami M, Miyoshi M and Yamashita H: Gut microbiota and host metabolism in liver cirrhosis. World J Gastroenterol. 21:11597–11608. 2015.PubMed/NCBI View Article : Google Scholar

2 

Yang R, Xu Y, Dai Z, Lin X and Wang H: The immunologic role of gut microbiota in patients with chronic HBV infection. J Immunol Res. 2018(2361963)2018.PubMed/NCBI View Article : Google Scholar

3 

Lu H, Wu Z, Xu W, Yang J, Chen Y and Li L: Intestinal microbiota was assessed in cirrhotic patients with hepatitis B virus infection. Intestinal microbiota of HBV cirrhotic patients. Microb Ecol. 61:693–703. 2011.PubMed/NCBI View Article : Google Scholar

4 

Jun DW, Kim KT, Lee OY, Chae JD, Son BK, Kim SH, Jo YJ and Park YS: Association between small intestinal bacterial overgrowth and peripheral bacterial DNA in cirrhotic patients. Dig Dis Sci. 55:1465–1471. 2010.PubMed/NCBI View Article : Google Scholar

5 

Usami M, Miyoshi M, Kanbara Y, Aoyama M, Sakaki H, Shuno K, Hirata K, Takahashi M, Ueno K, Hamada Y, et al: Analysis of fecal microbiota, organic acids and plasma lipids in hepatic cancer patients with or without liver cirrhosis. Clin Nutr. 32:444–451. 2013.PubMed/NCBI View Article : Google Scholar

6 

Dong X, Feng X, Liu J, Xu Y, Pan Q, Ling Z, Yu J, Yang J, Li L and Cao H: Characteristics of intestinal microecology during mesenchymal stem cell-based therapy for mouse acute liver injury. Stem Cells Int. 2019(2403793)2019.PubMed/NCBI View Article : Google Scholar

7 

Tremaroli V and Bäckhed F: Functional interactions between the gut microbiota and host metabolism. Nature. 489:242–249. 2012.

8 

Cani PD: Metabolism in 2013: The gut microbiota manages host metabolism. Nat Rev Endocrinol. 10:74–76. 2014.PubMed/NCBI View Article : Google Scholar

9 

Maynard CL, Elson CO, Hatton RD and Weaver CT: Reciprocal interactions of the intestinal microbiota and immune system. Nature. 489:231–241. 2012.PubMed/NCBI View Article : Google Scholar

10 

Viaud S, Saccheri F, Mignot G, Yamazaki T, Daillère R, Hannani D, Enot DP, Pfirschke C, Engblom C, Pittet MJ, et al: The intestinal microbiota modulates the anticancer immune effects of cyclophosphamide. Science. 342:971–976. 2013.PubMed/NCBI View Article : Google Scholar

11 

Fukuda S, Toh H, Hase K, Oshima K, Nakanishi Y, Yoshimura K, Tobe T, Clarke JM, Topping DL, Suzuki T, et al: Bifidobacteria can protect from enteropathogenic infection through production of acetate. Nature. 469:543–547. 2011.PubMed/NCBI View Article : Google Scholar

12 

Schroeder BO and Bäckhed F: Signals from the gut microbiota to distant organs in physiology and disease. Nat Med. 22:1079–1089. 2016.PubMed/NCBI View Article : Google Scholar

13 

Lin R, Zhou L, Zhang J and Wang B: Abnormal intestinal permeability and microbiota in patients with autoimmune hepatitis. Int J Clin Exp Pathol. 8:5153–5160. 2015.PubMed/NCBI

14 

Moen AEF, Lindstrøm JC, Tannæs TM, Vatn S, Ricanek P, Vatn MH and Jahnsen J: IBD-Character Consortium. The prevalence and transcriptional activity of the mucosal microbiota of ulcerative colitis patients. Sci Rep. 8(17278)2018.PubMed/NCBI View Article : Google Scholar

15 

Michalopoulos GK: Liver regeneration. J Cell Physiol. 213:286–300. 2007.PubMed/NCBI View Article : Google Scholar

16 

Michalopoulos GK: Hepatostat: Liver regeneration and normal liver tissue maintenance. Hepatology. 65:1384–1392. 2017.PubMed/NCBI View Article : Google Scholar

17 

Flye MW and Yu S: Augmentation of cell-mediated cytotoxicity following 50% partial hepatectomy. Transplantation. 49:581–587. 1990.PubMed/NCBI View Article : Google Scholar

18 

Cao H, Yu J, Xu W, Jia X, Yang J, Pan Q, Zhang Q, Sheng G, Li J, Pan X, et al: Proteomic analysis of regenerating mouse liver following 50% partial hepatectomy. Proteome Sci. 7(48)2009.PubMed/NCBI View Article : Google Scholar

19 

Brandt HH, Nißler V and Croner RS: The influence of liver resection on intrahepatic tumor growth. J Vis Exp. 110(e53946)2016.PubMed/NCBI View Article : Google Scholar

20 

Fadrosh DW, Ma B, Gajer P, Sengamalay N, Ott S, Brotman RM and Ravel J: An improved dual-indexing approach for multiplexed 16S rRNA gene sequencing on the Illumina MiSeq platform. Microbiome. 2(6)2014.PubMed/NCBI View Article : Google Scholar

21 

Callahan BJ, McMurdie PJ, Rosen MJ, Han AW, Johnson AJ and Holmes SP: DADA2: High-resolution sample inference from Illumina amplicon data. Nat Methods. 13:581–583. 2016.PubMed/NCBI View Article : Google Scholar

22 

Blaxter M, Mann J, Chapman T, Thomas F, Whitton C, Floyd R and Abebe E: Defining operational taxonomic units using DNA barcode data. Philos Trans R Soc Lond B Biol Sci. 360:1935–1943. 2005.PubMed/NCBI View Article : Google Scholar

23 

Bolyen E, Rideout JR, Dillon MR, Bokulich NA, Abnet CC, Al-Ghalith GA, Alexander H, Alm EJ, Arumugam M, Asnicar F, et al: Author correction: Reproducible, interactive, scalable and extensible microbiome data science using QIIME 2. Nat Biotechnol. 37(1091)2019.PubMed/NCBI View Article : Google Scholar

24 

Caporaso JG, Kuczynski J, Stombaugh J, Bittinger K, Bushman FD, Costello EK, Fierer N, Peña AG, Goodrich JK, Gordon JI, et al: QIIME allows analysis of high-throughput community sequencing data. Nat Methods. 7:335–336. 2010.PubMed/NCBI View Article : Google Scholar

25 

Segata N, Izard J, Waldron L, Gevers D, Miropolsky L, Garrett WS and Huttenhower C: Metagenomic biomarker discovery and explanation. Genome Biol. 12(R60)2011.PubMed/NCBI View Article : Google Scholar

26 

Langille MG, Zaneveld J, Caporaso JG, McDonald D, Knights D, Reyes JA, Clemente JC, Burkepile DE, Vega Thurber RL, Knight R, et al: Predictive functional profiling of microbial communities using 16S rRNA marker gene sequences. Nat Biotechnol. 31:814–821. 2013.PubMed/NCBI View Article : Google Scholar

27 

Parks DH, Tyson GW, Hugenholtz P and Beiko RG: STAMP: Statistical analysis of taxonomic and functional profiles. Bioinformatics. 30:3123–3124. 2014.PubMed/NCBI View Article : Google Scholar

28 

Liu HX, Rocha CS, Dandekar S and Wan YJ: Functional analysis of the relationship between intestinal microbiota and the expression of hepatic genes and pathways during the course of liver regeneration. J Hepatol. 64:641–650. 2016.PubMed/NCBI View Article : Google Scholar

29 

Crespo-Piazuelo D, Estellé J, Revilla M, Criado-Mesas L, Ramayo-Caldas Y, Óvilo C, Fernández AI, Ballester M and Folch JM: Characterization of bacterial microbiota compositions along the intestinal tract in pigs and their interactions and functions. Sci Rep. 8(12727)2018.PubMed/NCBI View Article : Google Scholar

30 

Samul D, Worsztynowicz P, Leja K and Grajek W: Beneficial and harmful roles of bacteria from the Clostridium genus. Acta Biochim Pol. 60:515–521. 2013.PubMed/NCBI

31 

Masumoto S, Terao A, Yamamoto Y, Mukai T, Miura T and Shoji T: Non-absorbable apple procyanidins prevent obesity associated with gut microbial and metabolomic changes. Sci Rep. 6(31208)2016.PubMed/NCBI View Article : Google Scholar

32 

Jiao X, Wang Y, Lin Y, Lang Y, Li E, Zhang X, Zhang Q, Feng Y, Meng X and Li B: Blueberry polyphenols extract as a potential prebiotic with anti-obesity effects on C57BL/6 J mice by modulating the gut microbiota. J Nutr Biochem. 64:88–100. 2019.PubMed/NCBI View Article : Google Scholar

33 

Reunanen J, Kainulainen V, Huuskonen L, Ottman N, Belzer C, Huhtinen H, de Vos WM and Satokari R: Akkermansia muciniphila adheres to enterocytes and strengthens the integrity of the epithelial cell layer. Appl Environ Microbiol. 81:3655–3662. 2015.PubMed/NCBI View Article : Google Scholar

34 

Belzer C and de Vos WM: Microbes inside-from diversity to function: The case of Akkermansia. ISME J. 6:1449–1458. 2012.PubMed/NCBI View Article : Google Scholar

35 

Anhê FF, Roy D, Pilon G, Dudonné S, Matamoros S, Varin TV, Garofalo C, Moine Q, Desjardins Y, Levy E and Marette A: A polyphenol-rich cranberry extract protects from diet-induced obesity, insulin resistance and intestinal inflammation in association with increased Akkermansia spp. population in the gut microbiota of mice. Gut. 64:872–883. 2015.PubMed/NCBI View Article : Google Scholar

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Shao Y, Jiang Y, Li H, Zhang F, Hu Z and Zheng S: Characteristics of mouse intestinal microbiota during acute liver injury and repair following 50% partial hepatectomy. Exp Ther Med 22: 953, 2021.
APA
Shao, Y., Jiang, Y., Li, H., Zhang, F., Hu, Z., & Zheng, S. (2021). Characteristics of mouse intestinal microbiota during acute liver injury and repair following 50% partial hepatectomy. Experimental and Therapeutic Medicine, 22, 953. https://doi.org/10.3892/etm.2021.10385
MLA
Shao, Y., Jiang, Y., Li, H., Zhang, F., Hu, Z., Zheng, S."Characteristics of mouse intestinal microbiota during acute liver injury and repair following 50% partial hepatectomy". Experimental and Therapeutic Medicine 22.3 (2021): 953.
Chicago
Shao, Y., Jiang, Y., Li, H., Zhang, F., Hu, Z., Zheng, S."Characteristics of mouse intestinal microbiota during acute liver injury and repair following 50% partial hepatectomy". Experimental and Therapeutic Medicine 22, no. 3 (2021): 953. https://doi.org/10.3892/etm.2021.10385
Copy and paste a formatted citation
x
Spandidos Publications style
Shao Y, Jiang Y, Li H, Zhang F, Hu Z and Zheng S: Characteristics of mouse intestinal microbiota during acute liver injury and repair following 50% partial hepatectomy. Exp Ther Med 22: 953, 2021.
APA
Shao, Y., Jiang, Y., Li, H., Zhang, F., Hu, Z., & Zheng, S. (2021). Characteristics of mouse intestinal microbiota during acute liver injury and repair following 50% partial hepatectomy. Experimental and Therapeutic Medicine, 22, 953. https://doi.org/10.3892/etm.2021.10385
MLA
Shao, Y., Jiang, Y., Li, H., Zhang, F., Hu, Z., Zheng, S."Characteristics of mouse intestinal microbiota during acute liver injury and repair following 50% partial hepatectomy". Experimental and Therapeutic Medicine 22.3 (2021): 953.
Chicago
Shao, Y., Jiang, Y., Li, H., Zhang, F., Hu, Z., Zheng, S."Characteristics of mouse intestinal microbiota during acute liver injury and repair following 50% partial hepatectomy". Experimental and Therapeutic Medicine 22, no. 3 (2021): 953. https://doi.org/10.3892/etm.2021.10385
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team