Characteristics of mouse intestinal microbiota during acute liver injury and repair following 50% partial hepatectomy

Shao,Yi; Jiang,Yuancong; Li,Hui; Zhang,Feng; Hu,Zhenhua; Zheng,Shusen

doi:10.3892/etm.2021.10385

Characteristics of mouse intestinal microbiota during acute liver injury and repair following 50% partial hepatectomy

Authors:
- Yi Shao
- Yuancong Jiang
- Hui Li
- Feng Zhang
- Zhenhua Hu
- Shusen Zheng
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Affiliations: Department of Hepatobiliary and Pancreatic Surgery, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang 310003, P.R. China, Key Laboratory of Organ Transplantation, Research Center for Diagnosis and Treatment of Hepatobiliary Diseases, Hangzhou, Zhejiang 310003, P.R. China
Published online on: July 5, 2021 https://doi.org/10.3892/etm.2021.10385
Article Number: 953
Copyright: © Shao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Dysbiosis of the gut microbiota has important roles in various diseases and pathological states of the host. However, the changes of the gut microbiota during partial hepatectomy (PH)‑induced acute liver injury have so far remained elusive. The present study investigated the gut microbiome and its related pathways following PH‑induced acute liver injury. A total of 50 male C57/BL6 mice were divided into a normal control (NC), sham‑operation and liver resection (LR) group (50% PH). Samples were collected at 3 and 14 days post‑operation to obtain specimens for the Sham3, Sham14, LR3 and LR14 groups (10 mice/group). Specimens of NC group (n=10) were obtained at the same time as those of Sham3 group. Alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were determined using an automatic chemical analyzer and the gut microbiota was assessed by 16S ribosomal RNA gene sequencing of small intestinal contents. The serum levels of ALT and AST in the LR3 group were significantly increased, while those in the LR14 group were decreased again to near‑normal levels. In the LR3 group, the operational taxonomic units, species richness (Chao1) and species diversity (Shannon and Simpson indices) were decreased, although without any significant difference. Furthermore, in the LR3 group, significant Cyanobacteria enrichment and Fusobacteria depletion compared with the NC and Sham3 groups was observed, while in the LR14 group, a significant depletion of the abundance of Verrucomicrobia, Chloroflexi and Deferribacteres compared to the LR3 group was obtained. The abundance of Firmicutes was increased in the LR3 group and decreased again in the LR14 group. However, the abundance of Bacteroidetes and Actinobacteria decreased in the LR3 group and increased again in the LR14 group. The alterations of the gut microbiota at the genus level were also revealed, as significant increases in Chloroplast, Curvibacter, Pelomonas, Ruminococcaceae UCG‑005 and Blautia and a sharp decrease in Akkermansia and Eubacterium coprostanoligenes were caused by acute liver injury. Furthermore, functional metagenome prediction was performed by Phylogenetic Investigation of Communities by Reconstruction of Unobserved States based on the Greengenes database, revealing alterations in signal transduction, transcription and cell motility, as well as metabolism of amino acids, lipids, glucose, cofactors and terpenoids, and xenobiotics pathways. An improved understanding of the structural and functional changes of the gut microbiota following 50% PH‑induced acute liver injury and repair may provide novel strategies for the recovery of hosts undergoing hepatectomy.

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