Open Access

Metabolites of intestinal microflora upregulate microRNA‑200c‑3p expression level to suppress airway epithelial inflammation via the IL6ST/JNK/STAT3 signaling pathway

  • Authors:
    • Linliang Hong
    • Huanhuan Huang
    • Bin Wu
  • View Affiliations

  • Published online on: July 15, 2021     https://doi.org/10.3892/etm.2021.10431
  • Article Number: 999
  • Copyright: © Hong et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Intestinal microfloras are involved in various types of cancer; however, there is a limited amount of research into the involvement of metabolites of intestinal microflora (MIM) in asthmatic airway epithelial cells (AECs). The present study was designed to reveal the functions and mechanisms of MIM in the asthmatic inflammation of AECs. House dust mite (HDM)‑induced asthma cell models were established and treated with mouse MIM. A MTT assay was used to investigate AEC viability, while reverse transcription‑quantitative PCR and western blot analysis were used to measure the expression levels of miR‑200c‑3p, IL6ST, JNK and STAT3 in asthmatic AECs. ELISA was used to measure the concentration of IL‑5 and IL‑6. Furthermore, the targeting relationship between microRNA(miR)‑200c‑3p and IL6ST was investigated using a luciferase reporter gene assay. Compared with normal human bronchial epithelial cells, HDM‑induced AECs had lower expression level of miR‑200c‑3p, higher mRNA and protein expression levels of IL6ST and an increase in IL‑5 and IL‑6 concentration. Both MIM and miR‑200c‑3p mimics suppressed the secretion of IL‑5 and L‑6 and promoted the proliferation of HDM‑induced AECs. MIM could also upregulate miR‑200c‑3p and downregulate IL6ST and proteins in the JNK/STAT3 pathway. IL6ST was found to be a downstream target of miR‑200c‑3p. Inhibition of miR‑200c‑3p reversed the suppression of asthmatic inflammation by MIM. In summary, MIM upregulated miR‑200c‑3p expression level to reduce the protein and mRNA expression levels of IL6ST and suppress its downstream JNK/STAT3 signaling pathway, therefore inhibiting the asthmatic inflammation of AECs.

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September-2021
Volume 22 Issue 3

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Hong L, Huang H and Wu B: Metabolites of intestinal microflora upregulate microRNA‑200c‑3p expression level to suppress airway epithelial inflammation via the IL6ST/JNK/STAT3 signaling pathway. Exp Ther Med 22: 999, 2021
APA
Hong, L., Huang, H., & Wu, B. (2021). Metabolites of intestinal microflora upregulate microRNA‑200c‑3p expression level to suppress airway epithelial inflammation via the IL6ST/JNK/STAT3 signaling pathway. Experimental and Therapeutic Medicine, 22, 999. https://doi.org/10.3892/etm.2021.10431
MLA
Hong, L., Huang, H., Wu, B."Metabolites of intestinal microflora upregulate microRNA‑200c‑3p expression level to suppress airway epithelial inflammation via the IL6ST/JNK/STAT3 signaling pathway". Experimental and Therapeutic Medicine 22.3 (2021): 999.
Chicago
Hong, L., Huang, H., Wu, B."Metabolites of intestinal microflora upregulate microRNA‑200c‑3p expression level to suppress airway epithelial inflammation via the IL6ST/JNK/STAT3 signaling pathway". Experimental and Therapeutic Medicine 22, no. 3 (2021): 999. https://doi.org/10.3892/etm.2021.10431