Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Experimental and Therapeutic Medicine
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-0981 Online ISSN: 1792-1015
Journal Cover
September-2021 Volume 22 Issue 3

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
September-2021 Volume 22 Issue 3

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article Open Access

Metabolites of intestinal microflora upregulate microRNA‑200c‑3p expression level to suppress airway epithelial inflammation via the IL6ST/JNK/STAT3 signaling pathway

  • Authors:
    • Linliang Hong
    • Huanhuan Huang
    • Bin Wu
  • View Affiliations / Copyright

    Affiliations: Department of Pediatrics, The First Affiliated Hospital of Fujian Medical University, Fuzhou, Fujian 350005, P.R. China
    Copyright: © Hong et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 999
    |
    Published online on: July 15, 2021
       https://doi.org/10.3892/etm.2021.10431
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Intestinal microfloras are involved in various types of cancer; however, there is a limited amount of research into the involvement of metabolites of intestinal microflora (MIM) in asthmatic airway epithelial cells (AECs). The present study was designed to reveal the functions and mechanisms of MIM in the asthmatic inflammation of AECs. House dust mite (HDM)‑induced asthma cell models were established and treated with mouse MIM. A MTT assay was used to investigate AEC viability, while reverse transcription‑quantitative PCR and western blot analysis were used to measure the expression levels of miR‑200c‑3p, IL6ST, JNK and STAT3 in asthmatic AECs. ELISA was used to measure the concentration of IL‑5 and IL‑6. Furthermore, the targeting relationship between microRNA(miR)‑200c‑3p and IL6ST was investigated using a luciferase reporter gene assay. Compared with normal human bronchial epithelial cells, HDM‑induced AECs had lower expression level of miR‑200c‑3p, higher mRNA and protein expression levels of IL6ST and an increase in IL‑5 and IL‑6 concentration. Both MIM and miR‑200c‑3p mimics suppressed the secretion of IL‑5 and L‑6 and promoted the proliferation of HDM‑induced AECs. MIM could also upregulate miR‑200c‑3p and downregulate IL6ST and proteins in the JNK/STAT3 pathway. IL6ST was found to be a downstream target of miR‑200c‑3p. Inhibition of miR‑200c‑3p reversed the suppression of asthmatic inflammation by MIM. In summary, MIM upregulated miR‑200c‑3p expression level to reduce the protein and mRNA expression levels of IL6ST and suppress its downstream JNK/STAT3 signaling pathway, therefore inhibiting the asthmatic inflammation of AECs.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Figure 6

View References

1 

Lambrecht BN and Hammad H: The immunology of asthma. Nat Immunol. 16:45–56. 2015.PubMed/NCBI View Article : Google Scholar

2 

Papi A, Brightling C, Pedersen SE and Reddel HK: Asthma. Lancet. 391:783–800. 2018.PubMed/NCBI View Article : Google Scholar

3 

Gon Y and Hashimoto S: Role of airway epithelial barrier dysfunction in pathogenesis of asthma. Allergol Int. 67:12–17. 2018.PubMed/NCBI View Article : Google Scholar

4 

Mertens TCJ, Karmouty-Quintana H, Taube C and Hiemstra PS: Use of airway epithelial cell culture to unravel the pathogenesis and study treatment in obstructive airway diseases. Pulm Pharmacol Ther. 45:101–113. 2017.PubMed/NCBI View Article : Google Scholar

5 

Mitchell PD and O'Byrne PM: Epithelial-derived cytokines in asthma. Chest. 151:1338–1344. 2017.PubMed/NCBI View Article : Google Scholar

6 

Zhang H, Sun Y, Rong W, Fan L, Cai Y, Qu Q, Gao Y and Zhao H: miR-221 participates in the airway epithelial cells injury in asthma via targeting SIRT1. Exp Lung Res. 44:272–279. 2018.PubMed/NCBI View Article : Google Scholar

7 

Kho AT, McGeachie MJ, Moore KG, Sylvia JM, Weiss ST and Tantisira KG: Circulating microRNAs and prediction of asthma exacerbation in childhood asthma. Respir Res. 19(128)2018.PubMed/NCBI View Article : Google Scholar

8 

Lam JK, Chow MY, Zhang Y and Leung SW: siRNA versus miRNA as therapeutics for gene silencing. Mol Ther Nucleic Acids. 4(e252)2015.PubMed/NCBI View Article : Google Scholar

9 

Ong J, Timens W, Rajendran V, Algra A, Spira A, Lenburg ME, Campbell JD, van den Berge M, Postma DS, van den Berg A, et al: Identification of transforming growth factor-beta-regulated microRNAs and the microRNA-targetomes in primary lung fibroblasts. PLoS One. 12(e0183815)2017.PubMed/NCBI View Article : Google Scholar

10 

Kivihall A, Aab A, Soja J, Sładek K, Sanak M, Altraja A, Jakiela B, Bochenek G and Rebane A: Reduced expression of miR-146a in human bronchial epithelial cells alters neutrophil migration. Clin Transl Allergy. 9(62)2019.PubMed/NCBI View Article : Google Scholar

11 

Liu Z, Zhang XH, Callejas-Diaz B and Mullol J: MicroRNA in United airway diseases. Int J Mol Sci. 17(716)2016.PubMed/NCBI View Article : Google Scholar

12 

Svitich OA, Sobolev VV, Gankovskaya LV, Zhigalkina PV and Zverev VV: The role of regulatory RNAs (miRNAs) in asthma. Allergol Immunopathol (Madr). 46:201–205. 2018.PubMed/NCBI View Article : Google Scholar

13 

Taka S, Tzani-Tzanopoulou P, Wanstall H and Papadopoulos NG: MicroRNAs in asthma and respiratory infections: Identifying common pathways. Allergy Asthma Immunol Res. 12:4–23. 2020.PubMed/NCBI View Article : Google Scholar

14 

Tang X, Wu F, Fan J, Jin Y, Wang J and Yang G: Posttranscriptional regulation of interleukin-33 expression by MicroRNA-200 in bronchial asthma. Mol Ther. 26:1808–1817. 2018.PubMed/NCBI View Article : Google Scholar

15 

Lustri BC, Sperandio V and Moreira CG: Bacterial chat: Intestinal metabolites and signals in host-microbiota-pathogen interactions. Infect Immun. 85:e00476–17. 2017.PubMed/NCBI View Article : Google Scholar

16 

Tuddenham S and Sears CL: The intestinal microbiome and health. Curr Opin Infect Dis. 28:464–470. 2015.PubMed/NCBI View Article : Google Scholar

17 

Wang G, Huang S, Wang Y, Cai S, Yu H, Liu H, Zeng X, Zhang G and Qiao S: Bridging intestinal immunity and gut microbiota by metabolites. Cell Mol Life Sci. 76:3917–3937. 2019.PubMed/NCBI View Article : Google Scholar

18 

Ver Heul A, Planer J and Kau AL: The human microbiota and asthma. Clin Rev Allergy Immunol. 57:350–363. 2019.PubMed/NCBI View Article : Google Scholar

19 

Xu Z, Tao J, Chen P, Chen L, Sharma S, Wang G and Dong Q: Sodium butyrate inhibits colorectal cancer cell migration by downregulating Bmi-1 through enhanced miR-200c expression. Mol Nutr Food Res. 62(e1700844)2018.PubMed/NCBI View Article : Google Scholar

20 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001.PubMed/NCBI View Article : Google Scholar

21 

Choi JY, Lee HY, Hur J, Kim KH, Kang JY, Rhee CK and Lee SY: TRPV1 blocking alleviates airway inflammation and remodeling in a chronic asthma murine model. Allergy Asthma Immunol Res. 10:216–224. 2018.PubMed/NCBI View Article : Google Scholar

22 

Jie Z, Xia H, Zhong SL, Feng Q, Li S, Liang S, Zhong H, Liu Z, Gao Y, Zhao H, et al: The gut microbiome in atherosclerotic cardiovascular disease. Nat Commun. 8(845)2017.PubMed/NCBI View Article : Google Scholar

23 

Mangiola F, Ianiro G, Franceschi F, Fagiuoli S, Gasbarrini G and Gasbarrini A: Gut microbiota in autism and mood disorders. World J Gastroenterol. 22:361–368. 2016.PubMed/NCBI View Article : Google Scholar

24 

Dong J, Tai JW and Lu LF: miRNA-microbiota interaction in gut homeostasis and colorectal cancer. Trends Cancer. 5:666–669. 2019.PubMed/NCBI View Article : Google Scholar

25 

Liu S, da Cunha AP, Rezende RM, Cialic R, Wei Z, Bry L, Comstock LE, Gandhi R and Weiner HL: The host shapes the gut microbiota via fecal MicroRNA. Cell Host Microbe. 19:32–43. 2016.PubMed/NCBI View Article : Google Scholar

26 

Teng Y, Ren Y, Sayed M, Hu X, Lei C, Kumar A, Hutchins E, Mu J, Deng Z, Luo C, et al: Plant-derived exosomal MicroRNAs shape the gut microbiota. Cell Host Microbe. 24:637–652.e8. 2018.PubMed/NCBI View Article : Google Scholar

27 

Chang CS and Kao CY: Current understanding of the gut microbiota shaping mechanisms. J Biomed Sci. 26(59)2019.PubMed/NCBI View Article : Google Scholar

28 

Bartel S, Schulz N, Alessandrini F, Schamberger AC, Pagel P, Theis FJ, Milger K, Noessner E, Stick SM, Kicic A, et al: Pulmonary microRNA profiles identify involvement of Creb1 and Sec14l3 in bronchial epithelial changes in allergic asthma. Sci Rep. 7(46026)2017.PubMed/NCBI View Article : Google Scholar

29 

Martinez-Nunez RT, Rupani H, Platé M, Niranjan M, Chambers RC, Howarth PH and Sanchez-Elsner T: Genome-wide posttranscriptional dysregulation by MicroRNAs in human asthma as revealed by frac-seq. J Immunol. 201:251–263. 2018.PubMed/NCBI View Article : Google Scholar

30 

Danarto R, Astuti I, Umbas R and Haryana SM: Urine miR-21-5p and miR-200c-3p as potential non-invasive biomarkers in patients with prostate cancer. Turk J Urol. 46:26–30. 2019.PubMed/NCBI View Article : Google Scholar

31 

Maolakuerban N, Azhati B, Tusong H, Abula A, Yasheng A and Xireyazidan A: MiR-200c-3p inhibits cell migration and invasion of clear cell renal cell carcinoma via regulating SLC6A1. Cancer Biol Ther. 19:282–291. 2018.PubMed/NCBI View Article : Google Scholar

32 

Bowman B: FADD and its phosphorylation mediate mitogenic signaling in mutant Kras tumors, 2015.

33 

Fan S, Sun JB, Li R, Song X and Li J: Lycopene protects myocardial ischemia injury through anti-apoptosis and anti-oxidative stress. Eur Rev Med Pharmacol Sci. 23:3096–3104. 2019.PubMed/NCBI View Article : Google Scholar

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Hong L, Huang H and Wu B: Metabolites of intestinal microflora upregulate microRNA‑200c‑3p expression level to suppress airway epithelial inflammation via the IL6ST/JNK/STAT3 signaling pathway. Exp Ther Med 22: 999, 2021.
APA
Hong, L., Huang, H., & Wu, B. (2021). Metabolites of intestinal microflora upregulate microRNA‑200c‑3p expression level to suppress airway epithelial inflammation via the IL6ST/JNK/STAT3 signaling pathway. Experimental and Therapeutic Medicine, 22, 999. https://doi.org/10.3892/etm.2021.10431
MLA
Hong, L., Huang, H., Wu, B."Metabolites of intestinal microflora upregulate microRNA‑200c‑3p expression level to suppress airway epithelial inflammation via the IL6ST/JNK/STAT3 signaling pathway". Experimental and Therapeutic Medicine 22.3 (2021): 999.
Chicago
Hong, L., Huang, H., Wu, B."Metabolites of intestinal microflora upregulate microRNA‑200c‑3p expression level to suppress airway epithelial inflammation via the IL6ST/JNK/STAT3 signaling pathway". Experimental and Therapeutic Medicine 22, no. 3 (2021): 999. https://doi.org/10.3892/etm.2021.10431
Copy and paste a formatted citation
x
Spandidos Publications style
Hong L, Huang H and Wu B: Metabolites of intestinal microflora upregulate microRNA‑200c‑3p expression level to suppress airway epithelial inflammation via the IL6ST/JNK/STAT3 signaling pathway. Exp Ther Med 22: 999, 2021.
APA
Hong, L., Huang, H., & Wu, B. (2021). Metabolites of intestinal microflora upregulate microRNA‑200c‑3p expression level to suppress airway epithelial inflammation via the IL6ST/JNK/STAT3 signaling pathway. Experimental and Therapeutic Medicine, 22, 999. https://doi.org/10.3892/etm.2021.10431
MLA
Hong, L., Huang, H., Wu, B."Metabolites of intestinal microflora upregulate microRNA‑200c‑3p expression level to suppress airway epithelial inflammation via the IL6ST/JNK/STAT3 signaling pathway". Experimental and Therapeutic Medicine 22.3 (2021): 999.
Chicago
Hong, L., Huang, H., Wu, B."Metabolites of intestinal microflora upregulate microRNA‑200c‑3p expression level to suppress airway epithelial inflammation via the IL6ST/JNK/STAT3 signaling pathway". Experimental and Therapeutic Medicine 22, no. 3 (2021): 999. https://doi.org/10.3892/etm.2021.10431
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team