Increased expression of cyclooxygenase‑2 in synovium tissues and synovial fluid from patients with knee osteoarthritis is associated with downregulated microRNA‑758‑3p expression
- Zhen Liu
- Jing Sun
- Tingting Liang
- Xiaonan Huang
Affiliations: Department of Joint Surgery, Heze Municipal Hospital, Heze, Shandong 274000, P.R. China, Department of Surgical Nursing, Heze Municipal Hospital, Heze, Shandong 274000, P.R. China, Department of Surgical Nursing, Dongda Hospital of Shanxian County, Heze, Shandong 274300, P.R. China, Department of Hand and Foot Microsurgery, Heze Municipal Hospital, Heze, Shandong 274000, P.R. China
- Published online on: July 15, 2021 https://doi.org/10.3892/etm.2021.10433
Copyright: © Liu
et al. This is an open access article distributed under the
terms of Creative
Commons Attribution License.
Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
This article is mentioned in:
Cyclooxygenase‑2 (COX‑2) is a common factor in inflammation, and its specific regulatory mechanism has not been fully elucidated. The present study aimed to investigate COX‑2 mRNA and protein expression levels in synovium tissues and synovial fluid from patients with knee osteoarthritis (KOA), and determine the molecular mechanism by which microRNA (miRNA/miR)‑758 regulates KOA via COX‑2. A total of 37 patients with KOA and 29 patients with acute knee trauma (control group) were enrolled in the present study. Reverse transcription‑quantitative PCR analysis was performed to detect miR‑758‑3p and COX‑2 mRNA expression, while western blotting and ELISA were performed to detect COX‑2 protein expression in synovium and synovial fluid, respectively. The dual‑luciferase reporter assay was performed to verify the interaction between miR‑758‑3p and the 3'‑untraslated region (UTR) of COX‑2 mRNA. Synovial cells were transfected with agomiR‑758‑3p, and the MTT assay was performed to assess cell proliferation. The results demonstrated that COX‑2 expression was higher in patients with KOA than those with acute knee trauma. Conversely, miR‑758‑3p expression was lower in patients with KOA than those with acute knee trauma. Notably, miR‑758‑3p interacted with the 3'‑UTR of COX‑2 mRNA to regulate its expression. Overexpression of miR‑758‑3p inhibited the expression and release of COX‑2, as well as the proliferation of human KOA synovial cells. Taken together, these results suggest that COX‑2 expression is upregulated in synovium tissues and synovial fluid from patients with KOA, which is associated with downregulated miR‑758‑3p expression. In addition, miR‑758‑3p affects the proliferation of synovial cells and the expression of relevant proteins in these cells, thus promoting the occurrence and development of KOA.