Open Access

Increased expression of cyclooxygenase‑2 in synovium tissues and synovial fluid from patients with knee osteoarthritis is associated with downregulated microRNA‑758‑3p expression

  • Authors:
    • Zhen Liu
    • Jing Sun
    • Tingting Liang
    • Xiaonan Huang
  • View Affiliations

  • Published online on: July 15, 2021     https://doi.org/10.3892/etm.2021.10433
  • Article Number: 1001
  • Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Cyclooxygenase‑2 (COX‑2) is a common factor in inflammation, and its specific regulatory mechanism has not been fully elucidated. The present study aimed to investigate COX‑2 mRNA and protein expression levels in synovium tissues and synovial fluid from patients with knee osteoarthritis (KOA), and determine the molecular mechanism by which microRNA (miRNA/miR)‑758 regulates KOA via COX‑2. A total of 37 patients with KOA and 29 patients with acute knee trauma (control group) were enrolled in the present study. Reverse transcription‑quantitative PCR analysis was performed to detect miR‑758‑3p and COX‑2 mRNA expression, while western blotting and ELISA were performed to detect COX‑2 protein expression in synovium and synovial fluid, respectively. The dual‑luciferase reporter assay was performed to verify the interaction between miR‑758‑3p and the 3'‑untraslated region (UTR) of COX‑2 mRNA. Synovial cells were transfected with agomiR‑758‑3p, and the MTT assay was performed to assess cell proliferation. The results demonstrated that COX‑2 expression was higher in patients with KOA than those with acute knee trauma. Conversely, miR‑758‑3p expression was lower in patients with KOA than those with acute knee trauma. Notably, miR‑758‑3p interacted with the 3'‑UTR of COX‑2 mRNA to regulate its expression. Overexpression of miR‑758‑3p inhibited the expression and release of COX‑2, as well as the proliferation of human KOA synovial cells. Taken together, these results suggest that COX‑2 expression is upregulated in synovium tissues and synovial fluid from patients with KOA, which is associated with downregulated miR‑758‑3p expression. In addition, miR‑758‑3p affects the proliferation of synovial cells and the expression of relevant proteins in these cells, thus promoting the occurrence and development of KOA.

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September-2021
Volume 22 Issue 3

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Liu Z, Sun J, Liang T and Huang X: Increased expression of cyclooxygenase‑2 in synovium tissues and synovial fluid from patients with knee osteoarthritis is associated with downregulated microRNA‑758‑3p expression. Exp Ther Med 22: 1001, 2021
APA
Liu, Z., Sun, J., Liang, T., & Huang, X. (2021). Increased expression of cyclooxygenase‑2 in synovium tissues and synovial fluid from patients with knee osteoarthritis is associated with downregulated microRNA‑758‑3p expression. Experimental and Therapeutic Medicine, 22, 1001. https://doi.org/10.3892/etm.2021.10433
MLA
Liu, Z., Sun, J., Liang, T., Huang, X."Increased expression of cyclooxygenase‑2 in synovium tissues and synovial fluid from patients with knee osteoarthritis is associated with downregulated microRNA‑758‑3p expression". Experimental and Therapeutic Medicine 22.3 (2021): 1001.
Chicago
Liu, Z., Sun, J., Liang, T., Huang, X."Increased expression of cyclooxygenase‑2 in synovium tissues and synovial fluid from patients with knee osteoarthritis is associated with downregulated microRNA‑758‑3p expression". Experimental and Therapeutic Medicine 22, no. 3 (2021): 1001. https://doi.org/10.3892/etm.2021.10433