Open Access

Knockdown of circ_0001883 may inhibit epithelial‑mesenchymal transition in laryngeal squamous cell carcinoma via the miR‑125‑5p/PI3K/AKT axis

Retraction in: /10.3892/etm.2024.12417

  • Authors:
    • Fu Chen
    • Zheng Lao
    • Haiyan Zhang
    • Jie Wang
    • Shengzi Wang
  • View Affiliations

  • Published online on: July 15, 2021     https://doi.org/10.3892/etm.2021.10440
  • Article Number: 1007
  • Copyright: © Chen et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Laryngeal squamous cell carcinoma (LSCC) is a malignant tumor with increasing incidence and poor prognosis. Circular RNAs (circRNAs) are known to modulate tumorigenesis and cancer development that may function through microRNAs (miRs). The aim of the present study was to investigate the functional roles of circ_0001883 in LSCC and the underlying molecular mechanism. The expression of circ_0001883 was upregulated and measured using reverse transcription‑quantitative PCR (RT‑qPCR) and RNase R. miR‑125b‑5p expression was downregulated in LSCC tissues and cells as determined using RT‑qPCR. Subsequently, knockdown of circ_0001883 inhibited LSCC cell migration, invasion and epithelial‑mesenchymal transition (EMT), which were tested by wound healing assays, Transwell assays and western blotting, respectively. Bioinformatics analysis predicted that circ_0001883 was a sponge of miR‑125b‑5p, which was verified using a dual‑luciferase reporter assay. Knockdown of circ_0001883 played a functional role by sponging miR‑125b‑5p. Additionally, circ_0001883 and miR‑125b‑5p influenced phosphorylation of PI3K and AKT, detected via western blotting. In an in vivo study, knockdown of circ_0001883 reduced tumor volume and weight in mice, along with enhanced miR‑125b‑5p and E‑cadherin expression levels, and decreased N‑cadherin, phosphorylated (p)‑PI3K/PI3K and p‑AKT/AKT ratios. In conclusion, knockdown of circ_0001883 inhibited cell migration, invasion and EMT of LSCC by sponging miR‑125b‑5p. This is hypothesized to be via the PI3K/AKT signaling pathway, which suggested that circ_0001883 has potential for LSCC therapy.
View Figures
View References

Related Articles

Journal Cover

September-2021
Volume 22 Issue 3

Print ISSN: 1792-0981
Online ISSN:1792-1015

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Chen F, Lao Z, Zhang H, Wang J and Wang S: Knockdown of circ_0001883 may inhibit epithelial‑mesenchymal transition in laryngeal squamous cell carcinoma via the miR‑125‑5p/PI3K/AKT axis Retraction in /10.3892/etm.2024.12417. Exp Ther Med 22: 1007, 2021
APA
Chen, F., Lao, Z., Zhang, H., Wang, J., & Wang, S. (2021). Knockdown of circ_0001883 may inhibit epithelial‑mesenchymal transition in laryngeal squamous cell carcinoma via the miR‑125‑5p/PI3K/AKT axis Retraction in /10.3892/etm.2024.12417. Experimental and Therapeutic Medicine, 22, 1007. https://doi.org/10.3892/etm.2021.10440
MLA
Chen, F., Lao, Z., Zhang, H., Wang, J., Wang, S."Knockdown of circ_0001883 may inhibit epithelial‑mesenchymal transition in laryngeal squamous cell carcinoma via the miR‑125‑5p/PI3K/AKT axis Retraction in /10.3892/etm.2024.12417". Experimental and Therapeutic Medicine 22.3 (2021): 1007.
Chicago
Chen, F., Lao, Z., Zhang, H., Wang, J., Wang, S."Knockdown of circ_0001883 may inhibit epithelial‑mesenchymal transition in laryngeal squamous cell carcinoma via the miR‑125‑5p/PI3K/AKT axis Retraction in /10.3892/etm.2024.12417". Experimental and Therapeutic Medicine 22, no. 3 (2021): 1007. https://doi.org/10.3892/etm.2021.10440