Knockdown of circ_0001883 may inhibit epithelial‑mesenchymal transition in laryngeal squamous cell carcinoma via the miR‑125‑5p/PI3K/AKT axis
- Fu Chen
- Zheng Lao
- Haiyan Zhang
- Jie Wang
- Shengzi Wang
Affiliations: Department of Radiation Oncology, Eye and ENT Hospital of Fudan University, Shanghai 200031, P.R. China, Radiotherapy Division, Department of Oral and Maxillofacial‑Head and Neck Oncology, Ninth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai 200011, P.R. China
- Published online on: July 15, 2021 https://doi.org/10.3892/etm.2021.10440
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et al. This is an open access article distributed under the
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Laryngeal squamous cell carcinoma (LSCC) is a malignant tumor with increasing incidence and poor prognosis. Circular RNAs (circRNAs) are known to modulate tumorigenesis and cancer development that may function through microRNAs (miRs). The aim of the present study was to investigate the functional roles of circ_0001883 in LSCC and the underlying molecular mechanism. The expression of circ_0001883 was upregulated and measured using reverse transcription‑quantitative PCR (RT‑qPCR) and RNase R. miR‑125b‑5p expression was downregulated in LSCC tissues and cells as determined using RT‑qPCR. Subsequently, knockdown of circ_0001883 inhibited LSCC cell migration, invasion and epithelial‑mesenchymal transition (EMT), which were tested by wound healing assays, Transwell assays and western blotting, respectively. Bioinformatics analysis predicted that circ_0001883 was a sponge of miR‑125b‑5p, which was verified using a dual‑luciferase reporter assay. Knockdown of circ_0001883 played a functional role by sponging miR‑125b‑5p. Additionally, circ_0001883 and miR‑125b‑5p influenced phosphorylation of PI3K and AKT, detected via western blotting. In an in vivo study, knockdown of circ_0001883 reduced tumor volume and weight in mice, along with enhanced miR‑125b‑5p and E‑cadherin expression levels, and decreased N‑cadherin, phosphorylated (p)‑PI3K/PI3K and p‑AKT/AKT ratios. In conclusion, knockdown of circ_0001883 inhibited cell migration, invasion and EMT of LSCC by sponging miR‑125b‑5p. This is hypothesized to be via the PI3K/AKT signaling pathway, which suggested that circ_0001883 has potential for LSCC therapy.